Synthesis of Freezing Tolerance Proteins in Leaves, Crown, and Roots during Cold Acclimation of Wheat

Abstract

Protein synthesis was studied in leaves, crown, and roots during cold hardening of freezing tolerant winter wheat (Triticum aestivum L. cv Fredrick and cv Norstar) and freezing sensitive spring wheat (T. aestivum L. cv Glenlea).

Cloning, Characterization, and Expression of a cDNA Encoding a 50-Kilodalton Protein Specifically Induced by Cold Acclimation in Wheat

“…A number of proteins and mRNAs have been identified that change in abundance during cold treatment (6,10,14,20,21). The isolation of cDNA clones regulated by low temperature has been reported in alfalfa (15,16), Arabidopsis (12), and barley (2, 7).…”

“…This induction was positively correlated with the capacity of plants and tissues to develop freezing tolerance (5,6,20). To elucidate which genes are induced, we constructed a cDNA library from cold-acclimated plants and screened it to isolate a cDNA induced in acclimated plants.…”

“…Using genotypes of wheat differing in their capacity to develop freezing tolerance, we have demonstrated (5,6,20) a correlation between the level of synthesis of a specific set of proteins and the capacity of each genotype to develop freezing tolerance. To understand the function of these proteins and the molecular basis for freezing tolerance, we began the isolation and analysis of the genes involved in the cold acclimation of wheat.…”

The Use of Callus Cultures for Searching Proteins Associated with Increased Freezing Tolerance During Cold Acclimation in Lonicera caerulea.

“…Many investigations have tried to elucidate the physiological and molecular events that take place during cold-acclimation (Levitt, 1980;Sakai and Larcher, 1987), and one approach has been to seek out proteins involved in increasing the freezing tolerance of the cells. It has been reported that low, nonfreezing temperatures induce the exclusive accumulation of some proteins in leaves and stems in plants such as Arabidopsis thaliana (Kurkela and Franck, 1990;Nordin et al, 1991;Gilmour et al, 1992), alfalfa (Mohapatra et al,1989), spinach , Brassica (Meza-Basso et al, 1986;Weretilnyk et al, 1993) and wheat (Perras and Sarhan, 1989). In woody plants grown in fields, the proteins that showed a specific increase in response to chilling treatment were found in the floral buds of blueberry (Muthalif and Rowland, 1994) and in the bark and xylem tissues of peach (Arora et al, 1992).…”

Estudio transcriptómico de los mecanismos implicados en la tolerancia inducida por el curado al daño de frío y por el etileno al colapso de la corteza en los frutos cítricos.

“…Teniendo en cuenta que la exposición al frío puede producir un aumento de la pérdida de agua por deshidratación, algunas de las respuestas que se producen durante la exposición a las bajas temperaturas de conservación pueden ser comunes a las que acontecen en condiciones de deshidratación, por una vía dependiente de ABA (Mohapatra et al, 1988;Perras y Sarhan, 1989). Un ejemplo de estas respuestas comunes lo constituye un grupo de genes que codifican proteínas dehidrinas, con función estabilizadora de membranas y de otras estructuras celulares, que se han relacionado con situaciones de estrés por dehridratación, pero también con la exposición a bajas temperaturas, la aplicación de ABA y con otros tipos de estrés que comportan una deshidratación de los tejidos como el estrés salino o la congelación (Baker et al, 1988;Bray, 1993;Close, 1997).…”