Synthesis of fructooligosaccharides (FosA) and inulin (InuO) by GH68 fructosyltransferases from Bacillus agaradhaerens strain WDG185

Kralj, Slavko; Leeflang, Chris; Sierra, Estefanía Ibáñez; Kempiński, Błażej; Alkan, Veli; Kolkman, Marc A. B.

doi:10.1016/j.carbpol.2017.09.069

Cited by 41 publications

(20 citation statements)

References 52 publications

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“…Such a pH optimum is rather unusual among the inulosucrases so far characterized [ 65 ], suggesting it may be an adaptation towards the specific environmental conditions in certain salt lakes, such as Tuz Lake. Most characterized inulosucrase enzymes show a more acidic pH optimum, with the exception of that of S. agaradhaerens [ 66 ] which shows a very broad pH optimum between 6 and 10, and the Bacillus sp. 217C-11 inulosucrase, with a pH optimum between 7 and 8 [ 67 ].…”

Section: Resultsmentioning

confidence: 99%

Functional and Molecular Characterization of the Halomicrobium sp. IBSBa Inulosucrase

et al. 2021

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Fructans are fructose-based (poly)saccharides with inulin and levan being the best-known ones. Thanks to their health-related benefits, inulin-type fructans have been under the focus of scientific and industrial communities, though mostly represented by plant-based inulins, and rarely by microbial ones. Recently, it was discovered that some extremely halophilic Archaea are also able to synthesize fructans. Here, we describe the first in-depth functional and molecular characterization of an Archaeal inulosucrase from Halomicrobium sp. IBSBa (HmcIsc). The HmcIsc enzyme was recombinantly expressed and purified in Escherichia coli and shown to synthesize inulin as proven by nuclear magnetic resonance (NMR) analysis. In accordance with the halophilic lifestyle of its native host, the enzyme showed maximum activity at very high NaCl concentrations (3.5 M), with specific adaptations for that purpose. Phylogenetic analyses suggested that Archaeal inulosucrases have been acquired from halophilic bacilli through horizontal gene transfer, with a HX(H/F)T motif evolving further into a HXHT motif, together with a unique D residue creating the onset of a specific alternative acceptor binding groove. This work uncovers a novel area in fructan research, highlighting unexplored aspects of life in hypersaline habitats, and raising questions about the general physiological relevance of inulosucrases and their products in nature.

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Section: Resultsmentioning

confidence: 99%

Functional and Molecular Characterization of the Halomicrobium sp. IBSBa Inulosucrase

et al. 2021

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“…Although almost all fructanogenic Bacillus species have been reported to produce levan-type fructans, FT enzymes from Salipaludibacillus agaradhaerens WDG185 (basionym: Bacillus agaradhaerens), another halotolerant species from the family Bacillaceae, were shown to synthesize short-chain (DP = 3-25) inulins (Kralj et al 2018), indicating that although a rare phenomenon, some Bacillaceae species may also be able to produce inulin-type fructans. The literature is rich with the reports of levan-producing Bacillus species, especially Bacillus subtilis (Salama et al 2019), Bacillus megaterium (Yu et al 2016) andB.…”

Section: Chemical Characterization Of the Epsmentioning

confidence: 99%

Fructanogenic traits in halotolerant Bacillus licheniformis OK12 and their predicted functional significance

Kırtel

Aydin

Öner

2021

J of Applied Microbiology

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Aims: Isolating a novel bacterial source of fructan from a saltern and analysis of its genome to better understand the possible roles of fructans in hypersaline environments. Methods and Results: Bacteria were isolated from crude salt samples originating from C ßamaltı Saltern in Western Turkey and screened for fructanogenic traits in high-salt and sucrose-rich selective medium. Exopolysaccharide accumulated in the presence of sucrose by isolate OK12 was purified and chemically characterized via HPLC, FT-IR and NMR, which revealed that it was a levan-type fructan (b-2,6 linked homopolymer of fructose). The isolate was taxonomically classified as Bacillus licheniformis OK12 through 16S rRNA gene and whole-genome sequencing methods. Strain OK12 harbours one levansucrase and two different levanase genes, which altogether were predicted to significantly contribute to intracellular glucose and fructose pools. The isolate could withstand 15% NaCl, and thus classified as a halotolerant. Conclusions: Fructanogenic traits in halotolerant B. licheniformis OK12 are significant due to predicted influx of glucose and fructose as a result of levan biosynthesis and levan hydrolysis, respectively. Significance and Impact of the Study: Fructans from the residents of hypersaline habitats are underexplored compounds and are expected to demonstrate physicochemical properties different from their non-halophilic counterparts. Revealing fructanogenic traits in the genome of a halotolerant bacterium brings up a new perspective in physiological roles of fructans.

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“…Because of easy handling, low cost and production of large quantity of enzyme, microbial FTase are preferred over plant FTase for prebiotics synthesis [20]. Therefore, fructosyltransferases have been isolated from different fungal and bacterial strains for commercial production of fructooligosaccharides [FOS] [21][22][23][24][25]. The type of FOS produced by different fructosyl transferases depend of the origin of the enzyme.…”

Section: Am J Biomed Sci and Resmentioning

confidence: 99%

A Note on Prebiotics Strategy to Cope with Current Health Challenges in Human Beings

Munawar¹

2020

AJBSR

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Synthesis of fructooligosaccharides (FosA) and inulin (InuO) by GH68 fructosyltransferases from Bacillus agaradhaerens strain WDG185

Cited by 41 publications

References 52 publications

Functional and Molecular Characterization of the Halomicrobium sp. IBSBa Inulosucrase

Functional and Molecular Characterization of the Halomicrobium sp. IBSBa Inulosucrase

Fructanogenic traits in halotolerant Bacillus licheniformis OK12 and their predicted functional significance

A Note on Prebiotics Strategy to Cope with Current Health Challenges in Human Beings

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