Synthesis of functional protein in liposome

Yu, Wei; Sato, Kazunao; Wakabayashi, Maki; Nakaishi, Tomoyuki; Ko-Mitamura, Elizabeth P.; Shima, Yasufumi; Urabe, Itaru; Yomo, Tetsuya

doi:10.1016/s1389-1723(01)80322-4

Cited by 183 publications

(79 citation statements)

References 20 publications

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“…Yu et al [68], for example, have reported the expression of a mutant GFP in lecithin liposomes. Large GFPexpressing vesicles, prepared by the film hydration method, were analysed using flow cytometry as well as confocal laser microscopy.…”

Section: Protein Expression In Liposomesmentioning

confidence: 99%

“…More recently, based on the initial report on the expression of functional protein in liposomes [68], Ishikawa et al [16] were able to design and produce experimentally a two-level cascading protein expression. A plasmid contain- A water-in-oil compartment system with water bubbles up to 50 μm Expression of GFP by mixing different compartments that are able to fuse with each other Pietrini and Luisi [49] A two-stage genetic network encapsulated in liposomes A genetic network in which the protein product of the first stage (T7 RNA polymerase) is required to drive the protein synthesis of the second stage (GFP) Ishikawa et al [16] E. coli cell-free expression system encapsulated in a phospholipid vesicle, which was transferred into a feeding solution containing ribonucleotides and amino acids…”

Section: Protein Expression In Liposomesmentioning

confidence: 99%

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Approaches to semi-synthetic minimal cells: a review

Luisi¹,

Ferri

Stano

2005

Naturwissenschaften

391

299

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Following is a synthetic review on the minimal living cell, defined as an artificial or a semi-artificial cell having the minimal and sufficient number of components to be considered alive. We describe concepts and experiments based on these constructions, and we point out that an operational definition of minimal cell does not define a single species, but rather a broad family of interrelated cell-like structures. The relevance of these researches, considering that the minimal cell should also correspond to the early simple cell in the origin of life and early evolution, is also explained. In addition, we present detailed data in relation to minimal genome, with observations cited by several authors who agree on setting the theoretical full-fledged minimal genome to a figure between 200 and 300 genes. However, further theoretical assumptions may significantly reduce this number (i.e. by eliminating ribosomal proteins and by limiting DNA and RNA polymerases to only a few, less specific molecular species). Generally, the experimental approach to minimal cells consists in utilizing liposomes as cell models and in filling them with genes/enzymes corresponding to minimal cellular functions. To date, a few research groups have successfully induced the expression of single proteins, such as the green fluorescence protein, inside liposomes. Here, different approaches are described and compared. Present constructs are still rather far from the minimal cell, and experimental as well as theoretical difficulties opposing further reduction of complexity are discussed. While most of these minimal cell constructions may represent relatively poor imitations of a modern full-fledged cell, further studies will begin precisely from these constructs. In conclusion, we give a brief outline of the next possible steps on the road map to the minimal cell.

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Section: Protein Expression In Liposomesmentioning

confidence: 99%

Section: Protein Expression In Liposomesmentioning

confidence: 99%

Approaches to semi-synthetic minimal cells: a review

Luisi¹,

Ferri

Stano

2005

Naturwissenschaften

391

299

View full text Add to dashboard Cite

show abstract

“…In general, yields were low. A coupled transcription/translation required for protein selection was so far only achieved in giant unilamellar liposomes, GUVs (diameter of more than 1 µm) [95,99,100]. In most of these investigations, all the components of the polymerization machinery were encapsulated along with their substrates.…”

Section: Liposome-entrapped Biopolymerizationmentioning

confidence: 99%

“…Second, compartmentalization permits accumulation of mRNA products up to concentrations which induce an accelerated translation of active protein compared to that in homogeneous medium [95]. Third, translated proteins are active [99]. Fourth, once encapsulated, the integrity of small compartments is preserved even under quite extreme conditions, such as high temperature cycling of a PCR reaction [97] and in the presence of degradative agents.…”

Section: Liposome-entrapped Biopolymerizationmentioning

confidence: 99%

“…Fourth, once encapsulated, the integrity of small compartments is preserved even under quite extreme conditions, such as high temperature cycling of a PCR reaction [97] and in the presence of degradative agents. For example, digestive enzymes in the external aqueous phase cannot interfere with the internalized biopolymer synthesis [93,99,101].…”

Section: Liposome-entrapped Biopolymerizationmentioning

confidence: 99%

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Catalysis in abiotic structured media: an approach to selective synthesis of biopolymers

Monnard

2005

CMLS, Cell. Mol. Life Sci.

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Micro- and nanoenvironments formed by amphiphile self-assembled structures, water-ice lattices and minerals have well-defined, repeating, chemical and physical properties that can be used for selective synthesis of biopolymers, such as RNAs and proteins. The advances made in the development of polymerization supported by these micro- and nanosystems are reviewed here. In particular, it is shown that these systems promote non-enzymatic biopolymerization, yielding long polymers whose sequence composition is determined by the interactions between monomers and the supporting environment. When used to compartmentalize enzymatic biopolymerization, micro- and nanostructures allow the implementation of molecular selection and evolution schemes, which are difficult in homogeneous medium, yielding very active molecules. Thus, micro- and nanoenvironment approaches to the synthesis and selection of biopolymers could be developed into a new biotechnological tool for the production of biopolymers with novel functions.

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Reactions in Dynamic Self‐Assemblies

Moulin

Giuseppone

2012

Supramolecular Chemistry

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The dynamic processes in self‐assemblies are involved in numerous domains that span throughout the modern aspects of chemical sciences, going from fundamental investigations into the origin of life to the most striking technological applications in medicine or electronics. This chapter aims at providing the reader with a brief overview of the concepts that link chemical reactivity with dynamic templates and catalysts made of large supramolecular assemblies. Specifically, we discuss kinetic and thermodynamic aspects of selected examples depending on (i) the nature of the matrix — that is, micelles, vesicles, liquid crystals, and gels; (ii) the nature of their constituents — that is, surfactants, block copolymers, bioactive macromolecules; and (iii) the nature of the chemical transformations that are performed — that is, reaction of small organic molecules, biomolecules, polymerizations, nanoparticle formation, and also reactions that involve the constituents of the matrix themselves as observed, for instance, in self‐reproducing systems. In each section, we combine original papers that paved the field together with more recent works of particular interest; several reviews are also referenced to help in accessing a more comprehensive literature.

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Synthesis of functional protein in liposome

Cited by 183 publications

References 20 publications

Approaches to semi-synthetic minimal cells: a review

Approaches to semi-synthetic minimal cells: a review

Catalysis in abiotic structured media: an approach to selective synthesis of biopolymers

Reactions in Dynamic Self‐Assemblies

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