Synthesis of fused glycoprotein D of Herpes simplex virus type 1 but not type 2 inhibits Escherichia coli hosts

Steinberg, Doron; Watson, R. J.; Maiese, William M.

doi:10.1016/0378-1119(86)90222-2

Cited by 6 publications

(1 citation statement)

References 17 publications

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“…It is known that a short (less than 15 amino acids) stretch of hydrophobic amino acid residues occurs in the signal peptide sequence or transmembrane anchor domain (Brosius, 1984;Steinberg et al, 1995). The signal peptide sequences of many viral glycoproteins have been implicated as possible causes of host toxicity and low expression level in E. coli (Brosius, 1984;Steinberg et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Recombinant Glycoprotein Vaccine Against HIRRV Infection.

Eou

Oh²,

Jung³

et al. 2001

Fish Pathol.

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ABSTRACT--Hirame rhabdovirus (HIRRV) was first reported from flounder (Paralichthys olivaceus) in Japan, and has recently been isolated from an aquaculture farm in the Tongyoung area of Korea. Total RNA isolated from RTG-2 cells infected with HIRRV was used in RT-PCR to obtain a cDNA coding for the glycoprotein of HIRRV Korean strain, CA-9703. Around half sized G protein in the C-terminal region was expressed as glutathione-S-transferase fusion protein, and used as viral vaccine against HIRRV. Flounder fry were vaccinated with low and high doses of vaccine by immersing them for 7 min in vaccine solution.After 1 month, the fish were challenged with HIRRV either by immersion or intraperitoneal injection. Specific losses of fish treated with low and high vaccine doses by intraperitoneal injection challenge were both 6.7%, in contrast to 43.3% in non-vaccinated fish. Losses of vaccinated fish by immersion chal lenge were 1.7% and 0% for low and high vaccine doses, respectively, and 15% for the non-vacci nated control.

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