Synthesis of glycosaminoglycans by human cervical fibroblasts in culture: effects of prostaglandian E2 and cyclic AMP

Carbonne, Bruno; Jannet, D.; Dallot, Emmanuelle; Pannier, E.; Ferré, Françoise; Cabrol, D.

doi:10.1016/s0301-2115(96)02536-5

Cited by 11 publications

(2 citation statements)

References 11 publications

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“…1). The unresponsiveness of intracellular cAMP levels to sulprostone/EP3 stimulation has been reported before for other cell types (48) and likely reflects the fact that EP3 receptors are mostly coupled to G i proteins. The weaker ability of the EP4 receptor to stimulate cAMP formation compared with EP2 has also been described (45, 50).…”

Section: Discussionsupporting

confidence: 66%

Prostaglandin E2 Differentially Modulates Proinflammatory/Prodestructive Effects of TNF-α on Synovial Fibroblasts via Specific E Prostanoid Receptors/cAMP

Kunisch

Jansen

Kojima

et al. 2009

The Journal of Immunology

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The present study investigated the influence of PGE2, E prostanoid (EP) receptors, and their signaling pathways on matrix metalloproteinase (MMP)-1 and IL-6 expression in synovial fibroblasts (SFs) from rheumatoid arthritis (RA) patients. RASFs expressed all four EP receptors, with selective induction of EP2 by TNF-α. TNF-α time-dependently increased intracellular cAMP/protein kinase A signaling (maximum, 6–12 h) and PGE2 secretion (maximum, 24 h). PGE2 and the EP2 agonists butaprost or ONO-AE1-259 ((16)-9-deoxy-9β-chloro-15-deoxy-16-hydroxy-17,17-trimethylene-19,20-didehydro PGE1), in turn, induced a rapid, time-dependent (maximum, 15–30 min) increase of cAMP. Additionally, cyclooxygenase-2 inhibition by NS-398 (N-(2-cyclohexyloxy-4-nitrophenyl)-methanesulfonamide) reduced the TNF-α-induced increase in IL-6 mRNA/protein, which was restored by stimulation with PGE2 or EP2, EP3, and EP4 agonists. In contrast, TNF-α-induced MMP-1 secretion was not influenced by NS-398 and diminished by PGE2 via EP2. Finally, 3-isobutyl-1-methylxanthine enhanced the effects of PGE2 on MMP-1, but not on IL-6 mRNA. In conclusion, PGE2 differentially affects TNF-α-induced mRNA expression of proinflammatory IL-6 and prodestructive MMP-1 regarding the usage of EP receptors and the dependency on cAMP. Although specific blockade of EP2 receptors is considered a promising therapeutic strategy in RA, opposite regulation of proinflammatory IL-6 and prodestructive MMP-1 by PGE2 via EP2 may require more complex approaches to successfully inhibit the cyclooxygenase-1/2 cAMP axis.

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Section: Discussionsupporting

confidence: 66%

Prostaglandin E2 Differentially Modulates Proinflammatory/Prodestructive Effects of TNF-α on Synovial Fibroblasts via Specific E Prostanoid Receptors/cAMP

Kunisch

Jansen

Kojima

et al. 2009

The Journal of Immunology

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“…Prostaglandins seem to promote cervical ECM changes by increasing water content and decreasing total collagen concentration [ 177 ]. Moreover, PGs stimulate GAG synthesis in cervical fibroblasts [ 178 ]. PGs strongly stimulate MMP activity, which makes it possible to cleave cross-links between cervical collagen fibrils [ 179 ].…”

Section: Regulation Of Cervical Ripeningmentioning

confidence: 99%

Signaling Pathways Regulating Human Cervical Ripening in Preterm and Term Delivery

Socha

Flis²,

Pietrus

et al. 2022

Cells

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At the end of gestation, the cervical tissue changes profoundly. As a result of these changes, the uterine cervix becomes soft and vulnerable to dilation. The process occurring in the cervical tissue can be described as cervical ripening. The ripening is a process derivative of enzymatic breakdown and inflammatory response. Therefore, it is apparent that cervical remodeling is a derivative of the reactions mediated by multiple factors such as hormones, prostaglandins, nitric oxide, and inflammatory cytokines. However, despite the research carried out over the years, the cellular pathways responsible for regulating this process are still poorly understood. A comprehensive understanding of the entire process of cervical ripening seems crucial in the context of labor induction. Greater knowledge could provide us with the means to help women who suffer from dysfunctional labor. The overall objective of this review is to present the current understanding of cervical ripening in terms of molecular regulation and cell signaling.

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Parturition

Mesiano

2015

Knobil and Neill's Physiology of Reproduction

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Synthesis of glycosaminoglycans by human cervical fibroblasts in culture: effects of prostaglandian E2 and cyclic AMP

Cited by 11 publications

References 11 publications

Prostaglandin E2 Differentially Modulates Proinflammatory/Prodestructive Effects of TNF-α on Synovial Fibroblasts via Specific E Prostanoid Receptors/cAMP

Prostaglandin E2 Differentially Modulates Proinflammatory/Prodestructive Effects of TNF-α on Synovial Fibroblasts via Specific E Prostanoid Receptors/cAMP

Signaling Pathways Regulating Human Cervical Ripening in Preterm and Term Delivery

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