Synthesis of Gold Nanoparticles with Polyamidoamine (Pamam) Generation 4 Dendrimer as Stabilizing Agent for CT Scan Contrast Agent

Sutriyo,; Mutalib, Abdul; Ristaniah,; Anwar, Effionora; Radji, Maksum; Pujiyanto, Anung; Purnamasari, Pony; Joshita, D; Adang, H G

doi:10.1002/masy.201550312

Cited by 13 publications

(4 citation statements)

References 10 publications

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“…The color change of the solution indicated the formation of monodisperse colloidal gold particles. The AuNPs were further characterized by determining their absorption maximum with a UV–Vis scan at 350–800 nm (CLARIO Starplus) and by determining the hydrodynamic radius of the colloidal solution through dynamic light scattering (Zetasizer) 25 – 28 .…”

Section: Methodsmentioning

confidence: 99%

Surface-enhanced Raman Spectroscopy in urinalysis of hypertension patients with kidney disease

Espinosa-Garavito,

Quiroz,

Galán-Freyle

et al. 2024

Sci Rep

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Arterial hypertension (AH) is a multifactorial and asymptomatic disease that affects vital organs such as the kidneys and heart. Considering its prevalence and the associated severe health repercussions, hypertension has become a disease of great relevance for public health across the globe. Conventionally, the classification of an individual as hypertensive or non-hypertensive is conducted through ambulatory blood pressure monitoring over a 24-h period. Although this method provides a reliable diagnosis, it has notable limitations, such as additional costs, intolerance experienced by some patients, and interferences derived from physical activities. Moreover, some patients with significant renal impairment may not present proteinuria. Accordingly, alternative methodologies are applied for the classification of individuals as hypertensive or non-hypertensive, such as the detection of metabolites in urine samples through liquid chromatography or mass spectrometry. However, the high cost of these techniques limits their applicability for clinical use. Consequently, an alternative methodology was developed for the detection of molecular patterns in urine collected from hypertension patients. This study generated a direct discrimination model for hypertensive and non-hypertensive individuals through the amplification of Raman signals in urine samples based on gold nanoparticles and supported by chemometric techniques such as partial least squares-discriminant analysis (PLS-DA). Specifically, 162 patient urine samples were used to create a PLS-DA model. These samples included 87 urine samples from patients diagnosed with hypertension and 75 samples from non-hypertensive volunteers. In the AH group, 35 patients were diagnosed with kidney damage and were further classified into a subgroup termed (RAH). The PLS-DA model with 4 latent variables (LV) was used to classify the hypertensive patients with external validation prediction (P) sensitivity of 86.4%, P specificity of 77.8%, and P accuracy of 82.5%. This study demonstrates the ability of surface-enhanced Raman spectroscopy to differentiate between hypertensive and non-hypertensive patients through urine samples, representing a significant advance in the detection and management of AH. Additionally, the same model was then used to discriminate only patients diagnosed with renal damage and controls with a P sensitivity of 100%, P specificity of 77.8%, and P accuracy of 82.5%.

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Section: Methodsmentioning

confidence: 99%

Surface-enhanced Raman Spectroscopy in urinalysis of hypertension patients with kidney disease

Espinosa-Garavito,

Quiroz,

Galán-Freyle

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The color change of the solution indicated the formation of monodisperse colloidal gold particles. The AuNPs were further characterized by determining their absorption maximum with a UV-Vis scan at 350-800 nm (CLARIO Starplus) and by determining the hydrodynamic radius of the colloidal solution through dynamic light scattering (Zetasizer) [22][23][24][25] .…”

Section: Synthesis and Characterization Of Aunpsmentioning

confidence: 99%

Surface-Enhanced Raman Spectroscopy in Urinalysis of Hypertension Patients with Kidney Disease

Espinosa-Garavito,

Quiroz,

Galán-Freyle

et al. 2023

Preprint

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Arterial hypertension (AH) is a multifactorial and asymptomatic disease that affects vital organs such as the kidneys and heart. Considering its prevalence and the associated severe health repercussions, hypertension has become a disease of great relevance for public health across the globe. Conventionally, the classification of an individual as hypertensive or nonhypertensive is conducted through ambulatory blood pressure monitoring over a 24-h period. Although this method provides a reliable diagnosis, it has notable limitations, such as additional costs, intolerance experienced by some patients, and interferences derived from physical activities. Moreover, some patients with significant renal impairment may not present proteinuria. Accordingly, alternative methodologies are applied for the classification of individuals as hypertensive or nonhypertensive, such as the detection of metabolites in urine samples through liquid chromatography or mass spectrometry. However, the high cost of these techniques limits their applicability for clinical use. Consequently, an alternative methodology was developed for the detection of molecular patterns in urine collected from hypertension patients. In this study, a direct discrimination model for hypertensive and nonhypertensive individuals was generated through the amplification of Raman signals in urine samples based on gold nanoparticles and supported by chemometric techniques such as partial least squares-discriminant analysis (PLS-DA). Specifically, 162 patient urine samples were used to create a PLS-DA model. These samples included 87 urine samples from patients diagnosed with hypertension and 75 samples from nonhypertensive volunteers. The PLS-DA model with 4 latent variables (LV) was used to classify the hypertensive patients with a calibration sensitivity (SenCal) of 89.2%, cross-validation sensitivity (SenCV) of 75.4%, prediction sensitivity (SenPred) of 86.4%, calibration specificity (SpeCal) of 86.0%, cross-validation specificity (SpeCV) of 77.2%, prediction specificity (SpePred) of 77.8%, calibration accuracy (AccCal) of 87.7%, cross-validation accuracy (AccCV) of 77.0%, and prediction accuracy (AccPred) of 82.5%. This study demonstrates the ability of surface-enhanced Raman spectroscopy to differentiate between hypertensive and nonhypertensive patients through urine samples, representing a significant advance in the detection and management of AH.

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“…RSV inhibits proliferation and causes toxic effect on 10ScNCr/23 mouse macrophages (IC50 Gold nanoparticle (AuNP) is metal nanoparticle which is well-known for its biocompatible, easy to prepare, and ready to modify with active ligand. AuNP has intense and specific surface plasmon resonance absorption, hence, easy to characterize [12][13][14][15]. Folic acid (FA) usage as active targeting moiety is based on specific overexpression of folate-binding protein found in epithelial cancer cells (MDA-MB-231, KB 3-1, and HeLa cells) [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Cellular Uptake Study and Cytotoxicity Study of Resveratrol-Gold-Peg-Folate (Rsv-Au-Peg-Fa) Nanoparticles on Hela Human Cervical Cancer Cell Line

2020

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Objective: This study aimed to evaluate the effectivity of resveratrol-gold-PEG-folate (RSV-Au-PEG-FA) nanoparticles formulation in resveratrol (RSV) targeted delivery and cytotoxicity effect on HeLa human cervical cancer cell line. Methods: Gold nanoparticles (AuNP) were used as carriers and folic acid (FA) was used as active targeting moiety, using polyethylene glycol-bis-amine (PEG-bis-amine) as linker. RSV-Au-PEG-FA nanoparticles were characterized by UV-Vis spectrophotometry, infrared spectroscopy, particle size analyzer (PSA), and transmission electron microscopy (TEM). Cellular uptake study was conducted by using fluorescence microscope. Cytotoxicity study was conducted by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: Cellular uptake study has shown that RSV-Au-PEG-FA nanoparticles are potential to be accumulated intracellularly in HeLa cells more than in Vero cells. Cytotoxicity study has shown RSV-Au-PEG-FA nanoparticles IC50 67.06±2.14 mM and RSV IC50 9.66±1.44 mM on HeLa cells Conclusion: RSV-Au-PEG-FA nanoparticles are potential to enhance RSV uptake by HeLa cells selectively.

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Synthesis of Gold Nanoparticles with Polyamidoamine (Pamam) Generation 4 Dendrimer as Stabilizing Agent for CT Scan Contrast Agent

Cited by 13 publications

References 10 publications

Surface-enhanced Raman Spectroscopy in urinalysis of hypertension patients with kidney disease

Surface-enhanced Raman Spectroscopy in urinalysis of hypertension patients with kidney disease

Surface-Enhanced Raman Spectroscopy in Urinalysis of Hypertension Patients with Kidney Disease

Cellular Uptake Study and Cytotoxicity Study of Resveratrol-Gold-Peg-Folate (Rsv-Au-Peg-Fa) Nanoparticles on Hela Human Cervical Cancer Cell Line

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