Synthesis of HÆmoglobin in Relation to the Maturation of Erythroid Cells

Hemoglobin Messenger RNA from Human Bone Marrow ISOLATION AND TRANSLATION IN HOMOZYGOUS AND HETEROZYGOUS β-THALASSEMIA

“…This disparity in alpha/beta chain synthesis was comparable to the disparity seen during whole cell incubation. Since 75-90% of total red cell hemoglobin is produced in the nucleated marrow erythroid cells (11), this observation was necessary to confirm the interpretation, based on studies with reticulocyte globin mRNA, that the molecular defect in homozygous beta thalassemia is a reduction in the amount of functional beta globin mRNA (9,10). Two interesting observations have been obtained from the study of double heterozygotes with sicklethalassemia.…”

Hemolysis of “stress” reticulocytes: a source of erythropoietic bilirubin formation

“…They do no exclude the possibility (41) that these cells survive normally and achieve normal size through loss of hemoglobinized cytoplasm, although radioautographic studies (40) support the first conclusion. Stress reticulocytes are released prematurely from the bone marrow, and are still actively engaged in hemoglobin synthesis (42). It is therefore not surprising that there is considerable labeling of hemoglobin heme in circulating cells only 24 hr after the administration of glycine-"C ( Table II), and that, with hemolysis of whole or part of these cells, a significant fraction of this labeled hemoglobin is rapidly degraded to bile pigment (Table II, Fig.…”

Intra- and interindividual variation in gene expression in human adipose tissue

“…Physiologically, it can be explained as follows. Hemoglobin is virtually exclusively present in red blood cells [10]. Consequently variation in blood content in the biopsy will result in apparent expression differences.…”