Synthesis of Haptens and Derivation of Monoclonal Antibodies for Immunoassay of the Phenylurea Herbicide Diuron

Karu, Alexander E.; Goodrow, Marvin H.; Schmidt, Doug; Hammock, Bruce D.; Bigelow, Michael W.

doi:10.1021/jf00038a014

Cited by 79 publications

(58 citation statements)

References 19 publications

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“…1a). pIMS 109-expressing anti-diuron scAb was prepared by replacing anti-atrazine scAb in pIMS 100 with a scAb derived from anti-diuron hybridoma cell line 275.1 (17,18).…”

Section: Scab Expression Vector Constructionmentioning

confidence: 99%

Escherichia coliSkp Chaperone Coexpression Improves Solubility and Phage Display of Single-Chain Antibody Fragments

Hayhurst

Harris

1999

Protein Expression and Purification

126

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“…1a). pIMS 109-expressing anti-diuron scAb was prepared by replacing anti-atrazine scAb in pIMS 100 with a scAb derived from anti-diuron hybridoma cell line 275.1 (17,18).…”

Section: Scab Expression Vector Constructionmentioning

confidence: 99%

Escherichia coliSkp Chaperone Coexpression Improves Solubility and Phage Display of Single-Chain Antibody Fragments

Hayhurst

Harris

1999

Protein Expression and Purification

126

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“…Immunoassay techniques for environmental contaminants were introduced in the early 1970s [17] and assays for a range of xenobiotics have been developed since, including PCBs [18], pesticides [19], herbicides [20], heavy metals [21], surfactants [22] and environmental estrogens [23]. A number of methods have been established for dioxins and related compounds, since routine monitoring of these compounds is difficult, time consuming and expensive; thus, screening methods that offer improvements in speed, sample throughput and cost are particularly desirable.…”

Section: Immunoassaysmentioning

confidence: 99%

The potential of mechanism-based bioanalytical tools in ecotoxicological exposure and effect assessment

Eggen

Segner

2003

Analytical and Bioanalytical Chemistry

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The current challenge to ecotoxicology is to develop tools that allow rapid and cost-efficient detection of those environmental chemicals or their combinations that are responsible for sublethal, chronic toxic effects in exposed organisms. Bioanalytical tools may meet these challenges, particularly if they are mechanism-based. Technically, bioanalytical tools allow rapid and cost-efficient analysis of environmental matrices. Mechanism-based, bioanalytical tools, however, do not only indicate that certain chemicals are there, but-and this is the major advantage of mechanism-based bioanalytical tools (MBBTs)-they indicate that chemicals with a specific mode of toxic action or a specific toxic potential are there. In this way MBBTs bridge exposure and effect assessment and help in a faster identification of the causative agent(s). Several principles of MBBTs, including immunoassays, enzyme inhibition assays, receptor assays and gene induction assays are briefly discussed and their application in processes such as bioassay-directed fractionation is illustrated. The focus of this manuscript is the analytical power of MBBTs in exposure and effect assessment. MBBTs have, however, a much broader potential and can support research on other challenges in ecotoxicology such as mixture effects or multiple effects caused by single pollutants or by various stresses simultaneously.

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“…Structural confirmation and purity assessments were made by melting-point (mp) determinations, proton nuclear mass resonance (NMR), and mass spectral analyses. The syntheses of compounds I (mp 103.5-104°C) and III (mp 155-156°C) have been previously reported (1,18). Compound IV was synthesized by adding a 10% molar excess of 3,4-dichloro amine (Aldrich) to 3,4-dichlorophenyl isocyanate dissolved in chloroform.…”

Section: Chemicals Compounds Listed Inmentioning

confidence: 99%

Evaluation of Fish Models of Soluble Epoxide Hydrolase Inhibition

Newman¹,

Denton²,

Morisseau³

et al. 2001

Environmental Health Perspectives

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Synthesis of Haptens and Derivation of Monoclonal Antibodies for Immunoassay of the Phenylurea Herbicide Diuron

Cited by 79 publications

References 19 publications

Escherichia coliSkp Chaperone Coexpression Improves Solubility and Phage Display of Single-Chain Antibody Fragments

Escherichia coliSkp Chaperone Coexpression Improves Solubility and Phage Display of Single-Chain Antibody Fragments

The potential of mechanism-based bioanalytical tools in ecotoxicological exposure and effect assessment

Evaluation of Fish Models of Soluble Epoxide Hydrolase Inhibition

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