Synthesis of heterogeneous enzyme–metal nanoparticle biohybrids in aqueous media and their applications in C–C bond formation and tandem catalysis

Filice, Marco; Marciello, Marzia; Morales, M.P.; Palomo, José M.

doi:10.1039/c3cc42475h

Cited by 129 publications

(137 citation statements)

References 21 publications

Supporting

Mentioning

137

Contrasting

Order By: Relevance

“…[36,37] To evaluatet his concept with nanoshells, Candida antarctica lipase Bw as selected as biocatalyst (Scheme 5). This idea was motivated by the well-established interaction/ reactivityo fg roups such ÀSH, ÀNH 2 ,a nd ÀOH, with metallic surfaces (Ag, Au, Pd,a nd Pt), which can facilitatet he lipase attachment to the bimetallic NSs.…”

Section: Bio-metallo-catalysts: Use Of Agau Agpt and Agpd Nss For Pmentioning

confidence: 99%

“…This idea was motivated by the well-established interaction/ reactivityo fg roups such ÀSH, ÀNH 2 ,a nd ÀOH, with metallic surfaces (Ag, Au, Pd,a nd Pt), which can facilitatet he lipase attachment to the bimetallic NSs. [36,37] To evaluatet his concept with nanoshells, Candida antarctica lipase Bw as selected as biocatalyst (Scheme 5). CALB has been employed as ab iocatalyst towards av ariety of reactions, and widely used to prepare chiral compounds.…”

Section: Bio-metallo-catalysts: Use Of Agau Agpt and Agpd Nss For Pmentioning

confidence: 99%

See 1 more Smart Citation

Bimetallic Nanoshells as Platforms for Metallo‐ and Biometallo‐Catalytic Applications

et al. 2015

View full text Add to dashboard Cite

The use of gold, silver, platinum and palladium for preparation of bimetallic nanoshells (AgAu, AgPt, and AgPdNSs, respectively) and their use for metallo‐ and bio‐metallo catalytic applications have been described. Bimetallic nanoshells (metallo‐catalysts) were employed for silane oxidation to silanols and hydrogen (H2) production. Fast and efficient oxidation of several silanes was observed after only 1 h at room temperature, by employing AgPd NSs as catalyst, acetone as solvent, and water as oxidant. Interestingly, bio‐metallo‐catalysts (NSs‐CALB) prepared from lipase attachment to the bimetallic nanoshells, displayed promising bi‐catalytic activities (enzymatic: transesterification; metallic: silane oxidation).

show abstract

Section: Bio-metallo-catalysts: Use Of Agau Agpt and Agpd Nss For Pmentioning

confidence: 99%

Section: Bio-metallo-catalysts: Use Of Agau Agpt and Agpd Nss For Pmentioning

confidence: 99%

Bimetallic Nanoshells as Platforms for Metallo‐ and Biometallo‐Catalytic Applications

et al. 2015

View full text Add to dashboard Cite

show abstract

“…These are very versatile enzymes with successful results in different processes using non-natural substrates [13] and they are commercially available in large amounts. Recently, these enzymes have been combined with metals in the preparation of several hybrids catalysts [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Design of Heterogeneous Hoveyda–Grubbs Second-Generation Catalyst–Lipase Conjugates

2016

View full text Add to dashboard Cite

Heterogeneous catalysts have been synthesized by the conjugation of Hoveyda-Grubbs second-generation catalyst with a lipase. The catalytic properties of the organometallic compound in solution were firstly optimized, evaluating the activity of Ru in the ring-closing metathesis of diethyldiallymalonate at 25 • C at different solvents and in the presence of different additives. The best result was found using tetrahydrofuran as a solvent. Some additives such as phenylboronic acid or polyetheneglycol slightly improved the activity of the Ru catalyst whereas others, such as pyridine or dipeptides affected it negatively. The organometallic compound immobilized on functionalized-surface materials activated with boronic acid or epoxy groups (around 50-60 µg per mg support) and showed 50% conversion at 24 h in the ring-closing metathesis. Cross-linked enzyme aggregates (CLEA's) of the Hoveyda-Grubbs second-generation catalyst with Candida antarctica lipase (CAL-B) were prepared, although low Ru catalyst was found to be translated in low conversion. Therefore, a sol-gel preparation of the Hoveyda-Grubbs second-generation and CAL-B was performed. This catalyst exhibited good activity in the metathesis of diethyldiallymalonate in toluene and in aqueous media. Finally, a new sustainable approach was used by the conjugation lipase-Grubbs in solid phase in aqueous media. Two strategies were used: one using lipase previously covalently immobilized on an epoxy-Sepharose support (hydrophilic matrix) and then conjugated with grubbs; and in the second, the free lipase was incubated with organometallic in aqueous solution and then immobilized on epoxy-Sepharose. The different catalysts showed excellent conversion values in the ring-closing metathesis of diethyldiallymalonate in aqueous media at 25 • C.

show abstract

“…The resulting enzyme-metal complex could work as a metalloenzyme with the catalytic activity originated from the introduced metal. Significantly, the surrounding proteins not only serve as protecting cage but also work as catalysts for distinct bioactivity (Engström et al, 2013; Filice et al, 2013; San et al, 2011). In one example, Bäckvall and coworkers created a hybrid catalyst consisting of CALB and Pd nanoparticles co-immobilized into the compartments of mesoporous silica for DKR of 1-phenylethylamine (Engström et al, 2013).…”

Section: Tandem Chemo-enzymatic Reactionsmentioning

confidence: 99%

Expanding the boundary of biocatalysis: design and optimization of in vitro tandem catalytic reactions for biochemical production

Wang

Ren

Zhao

2018

Critical Reviews in Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Biocatalysts have been increasingly used in the synthesis of fine chemicals and medicinal compounds due to significant advances in enzyme discovery and engineering. To mimic the synergistic effects of cascade reactions catalyzed by multiple enzymes in nature, researchers have been developing artificial tandem enzymatic reactions in vivo by harnessing synthetic biology and metabolic engineering tools. There is also growing interest in the development of one-pot tandem enzymatic or chemo-enzymatic processes in vitro due to their neat and concise catalytic systems and product purification procedures. In this review, we will briefly summarize the strategies of designing and optimizing in vitro tandem catalytic reactions, highlight a few representative examples, and discuss the future trend in this field.

show abstract

Synthesis of heterogeneous enzyme–metal nanoparticle biohybrids in aqueous media and their applications in C–C bond formation and tandem catalysis

Cited by 129 publications

References 21 publications

Bimetallic Nanoshells as Platforms for Metallo‐ and Biometallo‐Catalytic Applications

Bimetallic Nanoshells as Platforms for Metallo‐ and Biometallo‐Catalytic Applications

Design of Heterogeneous Hoveyda–Grubbs Second-Generation Catalyst–Lipase Conjugates

Expanding the boundary of biocatalysis: design and optimization of in vitro tandem catalytic reactions for biochemical production

Contact Info

Product

Resources

About