Synthesis of hierarchical nickel anchored on Fe₃O₄@SiO₂and its successful utilization to remove the abundant proteins (BHb) in bovine blood

Abstract

A hierarchical nickel shell anchored on silica coated magnetic Fe 3 O 4 @SiO 2 nanospheres (MNPs) has been designed and constructed that combined the capacity of effective adsorption for protein bovine hemoglobin (BHb) and selective deletion BHb from the bovine blood real sample. Firstly, -NH 2 modified silica/ magnetite nanoparticles (Fe 3 O 4 @SiO 2 -NH 2 ) were synthesized by the combination of a sol-gel method and refluxing process. The Fe 3 O 4 @SiO 2 -NH 2 composites revealed a core-shell structure, and the thickness of silica shell can be 10 easily adjusted by controlling the amount of ammonium solution. Then, hierarchical nickel shell were anchored on the surface of Fe 3 O 4 @SiO 2 -NH 2 by reducing Ni 2+ between the Fe 3 O 4 @SiO 2 -NH 2 solid and nickel solution with NaBH 4 . By taking advantages of the high affinity of Ni 2+ on the shell surface toward His-tagged proteins and the fast response toward an assistant magnet, the heteronanoparticles can be applied to selectively bind to and magnetically separate of BHb from the bovine blood. Significantly, it is expected that this approach can be extended to other bimolecular separation and purification.

Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex matrix of the silkworm fat body

“…MNPs can be produced through different methods, including grinding, thermal decomposition, microemulsion, chemical vapor deposition, coprecipitation, a reaction in a constrained environment, the polyol method, flow-injection synthesis, and sonolysis [ 14 , 16 – 18 ]. Methods for further surface modification have not been incorporated yet and are performed to modulate solubility, stability, internalization, or toxicity [ 14 , 16 , 17 , 19 ]. One of the most commonly used coating materials utilizes silica because of its efficiency, reduced toxicity, aggregation prevention, and hydrophilicity [ 14 , 19 ].…”

Ni-modified Magnetic Nanoparticles for Affinity Purification of His-tagged Proteins From the Complex Matrix of the Silkworm Fat Body

“…MNPs can be produced through different methods, including grinding, thermal decomposition, microemulsion, chemical vapor deposition, coprecipitation, reaction in a constrained environment, the polyol method, ow-injection synthesis and sonolysis [13][14][15][16]. Methods for further surface modi cation have not been incorporated yet and are performed to modulate solubility, stability, internalization, or toxicity [13][14][15]17]. One of the most commonly used coating materials utilizes silica because of its e ciency, reduced toxicity, prevention of aggregation, and hydrophilicity [15,17].…”

“…Methods for further surface modi cation have not been incorporated yet and are performed to modulate solubility, stability, internalization, or toxicity [13][14][15]17]. One of the most commonly used coating materials utilizes silica because of its e ciency, reduced toxicity, prevention of aggregation, and hydrophilicity [15,17]. In terms of toxicity, it is commonly assumed that iron nanoparticles are noncytotoxic and safe for use at a concentration of 100 µg/ml.…”

“…It was shown that the latter can be a safe and effective way to remove haze-forming proteins [18]; the surfaces of the MNPs were modi ed with amine, carboxyl, or oxazoline functional groups. Silicacoated iron magnetic nanoparticles functionalized with a nickel shell were used to remove the abundant protein hemoglobin from bovine blood [17]. Nickel has a high a nity for the His-tag and for proteins with a high number of histidine residues.…”

“…Nickel has a high a nity for the His-tag and for proteins with a high number of histidine residues. Therefore, bovine hemoglobin was successfully and selectively reduced, even if the level of reduction in the diluted bovine blood sample was low due to protein-protein interactions [17].…”