Synthesis of KCl-doped lignin carbon dots nanoenzymes for colorimetric sensing glutathione in human serum

Heterojunction MnO2-nanosheet-decorated Ag nanowires with enhanced oxidase-like activity for the sensitive dual-mode detection of glutathione

“…As described in the literature, the mechanism of colorimetry for GSH detection is initiated by reactive oxygen species (ROS), which then oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) to form blue-colored oxidized TMB (oxTMB), while the addition of GSH causes the color to fade. 13,14 However, most of these approaches face problems, such as the requirement of high H 2 O 2 concentrations as a substrate to generate ROS. Since H 2 O 2 is susceptible to thermal decomposition, it is important to design oxidase-like enzymes whose catalytic activity does not rely on H 2 O 2 .…”

Tetrabutylammonium-chloride-glycerol of deep eutectic solvent functionalized MnO2: a novel mimic enzyme for the quantitative and qualitative colorimetric detection of l-cysteine

“…1 shows the synthetic route for the [N 4444 ]Cl-G. The prepared DES was characterized by 1 H NMR, 13 C NMR, and FT-IR (as also can be seen in our previous research 30 ). The synthesis of [N 4444 ]Cl-G/ MnO 2 can be briefly described as follows: 1 mL of [N 4444 ]Cl-G and 4 mL of methanol were put into a 50 mL round-bottom flask, and then MnO 2 nanosheets (100 mg) were added to the above mixed solution.…”

“…In recent years, due to the advantages of its simple operation, there is no need for expensive instruments, visible detection by the naked eye, and the nanozyme-based colorimetric method has gradually attracted increasing attention and has been widely used in the field of analysis and detection. 7 To date, research on the reported oxidase mimics as colorimetric materials has mainly focused on metal nanoclusters/nanoparticles, 8,9 metal–organic frameworks, 10 graphene oxide, 11 iron oxides, 12 carbon dots, 13 etc . MnO 2 nanosheet, a type of transition metal oxide, has low toxicity and good biocompatibility, rich redox chemistry, and excellent catalytic and oxidase-like activity.…”

Design of carbon dots as nanozymes to mediate redox biological processes

“…For example, Cu and Zn covalently doped CDs exhibit higher electron abundance and have good electron transfer ability and stability. 50 Chen et al 68 successfully synthesized KCl-doped lignin CD-based nanozymes (K,Cl-LCDs) via a hydrothermal method, and constructed a glutathione sensor (Fig. 2c).…”