Synthesis of L-tyrosine from pyruvate, ammonia and phenol by crystalline tyrosine phenol lyase

Purification and Characterization of Membrane-bound Quinoprotein Quinate Dehydrogenase

“…Synthesis of L-tryptophan by L-tryptophan synthase or by a reverse reaction of L-tryptophanase and L-tyrosine by L-tyrosine phenol lyase from phenol, ammonia, and pyruvate, are typical examples. 16,17) Cinchona was used for production of quinine hydrochloride, a potent anti-malarial agent, and quinate came out as a byproduct in quinine-hydrochloride manufacturing. In order to develop the intermediates in the shikimate pathway, quinate dehydrogenase, theˆrst enzyme leading to the pathway from quinate must be characterized.…”

Enzymatic preparation of L‐tryptophan and 5‐hydroxy‐L‐tryptophan

“…In recent studies, we [9] proved that /3-tyrosine catalyze the synthesis of L-tyrosine from pyruvate, ammonia and phenol, apparently by the reversal of a,/5elimina-tion reaction. In appropriate studieb, it has been proved that the crystalline tryptophanase from E. coli [lo] and Proteus rettgeri [ 1 l] also catalyzes the synthesis of L-tryptophan by the reversal of &elmination reaction; at rates similar to the forward reaction.…”

Hyperproduction of 3,4-Dihydroxyphenyl-L-alanine (L-Dopa) UsingErwinia herbicolaCells Carrying a Mutant Transcriptional Regulator TyrR

“…This reaction is reversible, and when catechol is substituted for phenol, L-dopa is produced. 5) Practically, Erwinia herbicola cells with high Tpl activity are prepared by cultivation in a medium supplemented with L-tyrosine, harvested by centrifugation, and then transferred to the reactor together with the substrates (pyruvate, ammonia, and catechol). The synthesized L-dopa precipitates due to its low solubility, which favors the reverse reaction to proceed.…”