1988
DOI: 10.1002/aja.1001830304
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Synthesis of lens capsule and plasma membrane glycoproteins by lens epithelial cells and fibers in the rat

Abstract: The lens of the eye possesses a capsule which is a greatly hypertrophied basement membrane. To investigate the synthesis of glycoproteins destined for this capsule, 3H-fucose was injected into the vitreous body of intact rats weighing approximately 200 gm. The animals were killed from 10 min to 14.5 months later, and their lenses were processed for electron microscope radioautography. At 10 min after injection, more than 58% of the silver grains were localized to the Golgi apparatus of the lens epithelial cell… Show more

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Cited by 37 publications
(6 citation statements)
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“…In the embryo, both lens epithelial and fiber cells synthesize and secrete basement membrane molecules facilitating the growth of the anterior, posterior, and equatorial lens capsule. Pulse labeling studies have demonstrated that the embryonic capsule consists of successive layers of lamellae which first form adjacent to lens cells, then are buried within the capsule as new layers of basement membrane are laid down around the rapidly growing embryonic lens (Haddad and Bennett, 1988; Parmigiani and McAvoy, 1984; Parmigiani and McAvoy, 1991; Young and Ocumpaugh, 1966). By 15.5 dpc in the mouse, the posterior capsule has thickened to approximately 350 nm and distinct lamellae are observable by transmission electron microscopy (Csato, 1989).…”
Section: Lens Capsule Developmentmentioning
confidence: 99%
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“…In the embryo, both lens epithelial and fiber cells synthesize and secrete basement membrane molecules facilitating the growth of the anterior, posterior, and equatorial lens capsule. Pulse labeling studies have demonstrated that the embryonic capsule consists of successive layers of lamellae which first form adjacent to lens cells, then are buried within the capsule as new layers of basement membrane are laid down around the rapidly growing embryonic lens (Haddad and Bennett, 1988; Parmigiani and McAvoy, 1984; Parmigiani and McAvoy, 1991; Young and Ocumpaugh, 1966). By 15.5 dpc in the mouse, the posterior capsule has thickened to approximately 350 nm and distinct lamellae are observable by transmission electron microscopy (Csato, 1989).…”
Section: Lens Capsule Developmentmentioning
confidence: 99%
“…Postnatally, both the lens epithelium and fiber cells continue to deposit matrix molecules to the inner surface of the thickening capsule (Haddad and Bennett, 1988; Young and Ocumpaugh, 1966). Additionally, metabolic pulse labeling and copper reabsorption following lenticular chalcosis studies of the capsule have demonstrated that its matrix turns over extremely slowly, with a turnover rate measuring in months and years (Haddad and Bennett, 1988; Seland, 1976; Young and Ocumpaugh, 1966) compared to the hours measured for other basement membranes (Beavan et al, 1989; Dunsmore et al, 1995; Young and Ocumpaugh, 1966).…”
Section: Lens Capsule Developmentmentioning
confidence: 99%
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“…This raises the question as to the cellular source of the bFGF found in the lens capsule. Is it derived from the lens cells which synthesise the capsule (Haddad and Bennett, 1988) or is it released from the retinal ganglion cells and sequestered by the HSPG in the lens capsule? Although we have shown by Western analysis that bFGF can be detected in adult bovine lens cells (Schulz et al, 1993) and bFGF mRNA has been detected in cultured lens epithelium (Schweigerer et al, 1988), so far we have not detected cell-associated bFGF by immunohistochemistry in the developing lens in this or previous studies (de Iongh and McAvoy, 1992;Lovicu and McAvoy, 1993).…”
Section: Discussionmentioning
confidence: 99%
“…The contribution of radioautography to the clarification of the role of the Golgi apparatus in protein secretion was of crucial importance and the experimental design became less troublesome and more reliable with the availability of radioactive fucose (36)(37)(38)(39). There are detailed studies using electron microscopic radioautography to clarify the cellular process of glycoprotein secretion in some particular cell types, such as the thyroid follicular cells (40), ameloblasts (41), odontoblasts (42) and osteoblasts (43), the secretory neurons of the supraoptic and paraventricular nuclei (44), the acinar (45) and the endocrine (46) cells of the pancreas, the lens epithelium (47), the epithelial cells lining the granular ducts of the submandibular glands (48) and the uterine tubes (49) of the mouse, among others. All the above studies were performed using [ 3 H]-fucose for labeling the newly synthesized glycoproteins.…”
Section: Resultsmentioning
confidence: 99%