Synthesis of Luciferyl Coenzyme A: A Bioluminescent Substrate for Firefly Luciferase in the Presence of AMP

Abstract

Licht auf Luciferase: Luciferyl‐Coenzym A (1) wurde synthetisiert, und es zeigte sich, dass Luciferase die Lichterzeugung aus Luciferyl‐Coenzym A und AMP katalysiert. Diese Befunde eröffnen die Aussicht auf neue bioanalytische Methoden und sind ein starker Beleg für die evolutionäre Beziehung zwischen Glühwürmchen‐Luciferase und Acyl‐CoA‐Synthetasen.

Aminoluciferins as Functional Bioluminogenic Substrates of Firefly Luciferase

“…Recently, bioluminescence systems have been applied to detect other events, such as proteinprotein interactions and protease activity, by modification of luciferase. [6][7][8][9][10][11] On the other hand, functional luciferin analogues, the luminescence properties of which are changed dramatically by specific activity, for instance, b-galactosi-Abstract: Firefly luciferase is widely used as a reporter gene in assays to study gene expression, gene delivery, and so on because of its extremely high signal-to-noise ratio. The availability of a range of bioluminogenic substrates would greatly extend the applicability of the luciferin-luciferase system.…”

“…Characteristic advantages of bioluminescence systems include a high quantum yield (e.g., the recently reported value of Φ bl =0.41)5 without excitation light and extremely high signal‐to‐noise ratios compared with other imaging techniques, such as fluorescence, even in vivo. Recently, bioluminescence systems have been applied to detect other events, such as protein–protein interactions and protease activity, by modification of luciferase 6–11. On the other hand, functional luciferin analogues, the luminescence properties of which are changed dramatically by specific activity, for instance, β‐galactosidase,12 phosphatase,13 glutathione S‐transferase,14 and lactamase15 activity, have also been reported as highly sensitive indicators for target molecules.…”