Synthesis of luminescent lanthanide complexes within crosslinked protein crystal matrices

Zhang, Yu; Zhang, Xiaoting; Tang, Jianguo; Snow, Christopher D.; Sun, Guotao; Kowalski, Ann E.; Hartje, Luke F.; Zhao, Ning; Wang, Yao; Belfiore, Laurence A.

doi:10.1039/c8ce00318a

Cited by 9 publications

(14 citation statements)

References 54 publications

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“…Of the available confocal laser wavelengths, [(Eu(TTA) 3 phen] was best excited at 405 nm. 11 We therefore used a 405 nm laser to observe the crystal interior via z-slices collected at 2 μm intervals (Figure 4). In addition to the qualitative uniformity of the fluorescence images, there was quantitative uniformity.…”

Section: Crystal Growth and Designmentioning

confidence: 99%

“…6−9 Our research groups are also interested in synthesizing hybrid protein−inorganic crystals. 10,11 One important difference is that our nonbiological components are luminescent rare earth complexes. In solution, lanthanide complexes (LCs) offer very useful photonics properties such as a narrow emission band, large Stokes shift, and long luminescence lifetime.…”

Section: ■ Introductionmentioning

confidence: 99%

“…A final key technical aspect of this work is the refinement of methods for synthesizing LC inside of pre-existing protein crystals rather than diffusing intact LC into the crystals or attempting to incorporate LC during crystal growth. 11…”

Section: ■ Introductionmentioning

confidence: 99%

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Drug Sensing Protein Crystals Doped with Luminescent Lanthanide Complexes

Sun

Tang

Snow

et al. 2019

Crystal Growth & Design

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Hen egg white lysozyme (HEWL) crystals are a model system for protein crystallization due to economical production and facile crystal growth. Here we grew small, rod-like HEWL crystals and proceeded with stepwise synthesis to dope the protein crystals with luminescent lanthanide complexes [(Eu(TTA)3phen] (TTA = 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedionato, phen = 1,10-phenanthroline). Several compounds (coumarin, tinidazole, and acridine orange) were observed to quench the lanthanide complexes in solution or embedded within crystals. Coumarin is a precursor for anticoagulant drugs and tinidazole is used to combat bacterial or protozoan infections. In contrast, acridine orange is a widely used, versatile fluorescent dye used to stain acidic vacuoles, RNA, and DNA in living cells. These results suggest that protein crystals may provide a feasible matrix for entrapping luminescent species with practical biosensing applications.

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Section: Crystal Growth and Designmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Drug Sensing Protein Crystals Doped with Luminescent Lanthanide Complexes

Sun

Tang

Snow

et al. 2019

Crystal Growth & Design

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“…To do this, they used cross‐linked lysozyme crystals to template the assembly of a synthetic hydrogel while monitoring the gel synthesis process and crystal stability via X‐ray diffraction. In addition to templating hydrogels, protein crystals have been used as molds during the synthesis of both quantum dots (Wei et al, ) and carbon dots (England, Patil, & Mann, ) with tunable fluorescence, or to grow and coordinate gold nanoclusters (Kowalski et al, ; Liang et al, ; M. Liu et al, ; Wei et al, ; Wei & Lu, ) and luminescent lanthanide complexes (Y. Zhang et al, ). There have been many other accounts of stabilized protein crystals being utilized as effective biotemplating scaffolds and catalytic vessels for the assembly of organometallic complexes and biohybrid materials.…”

Section: Applications In Biotechnologymentioning

confidence: 99%

Protein crystal based materials for nanoscale applications in medicine and biotechnology

Hartje

Snow

2018

WIREs Nanomed Nanobiotechnol

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The porosity, order, biocompatibility, and chirality of protein crystals has motivated interest from diverse research domains including materials science, biotechnology, and medicine. Porous protein crystals have the unusual potential to organize guest molecules within highly ordered scaffolds, enabling applications ranging from biotemplating and catalysis to biosensing and drug delivery. Significant research has therefore been directed toward characterizing protein crystal materials in hopes of optimizing crystallization, scaffold stability, and application efficacy. In this overview article, we describe recent progress in the field of protein crystal materials with special attention given to applications in nanomedicine and nanobiotechnology.

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“…As already mentioned, − the tailoring of sequence composition to guide the assembly or the surface porous properties is also a focus of research . Several research groups have explored the use of protein crystals as biotemplates, the functionalization of protein crystals with metal ions, complexes, and nanoparticles, as reaction vessels, , trapping different organic molecules including dyes and luminescent compounds, , or even for in situ synthesis of luminescent compounds. − The entrapment of CdS quantum dots within lysozyme crystals enhances their fluorescence, which can also be modulated …”

Section: Introductionmentioning

confidence: 99%

Production of Cross-Linked Lipase Crystals at a Preparative Scale

Fernández-Penas

Verdugo‐Escamilla

Martínez‐Rodríguez

et al. 2021

Crystal Growth & Design

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The autoimmobilization of enzymes via cross-linked enzyme crystals (CLECs) has regained interest in recent years, boosted by the extensive knowledge gained in protein crystallization, the decrease of cost and laboriousness of the process, and the development of potential applications. In this work, we present the crystallization and preparative-scale production of reinforced cross-linked lipase crystals (RCLLCs) using a commercial detergent additive as a raw material. Bulk crystallization was carried out in 500 mL of agarose media using the batch technique. Agarose facilitates the homogeneous production of crystals, their cross-linking treatment, and their extraction. RCLLCs were active in an aqueous solution and in hexane, as shown by the hydrolysis of p -nitrophenol butyrate and α-methylbenzyl acetate, respectively. RCLLCs presented both high thermal and robust operational stability, allowing the preparation of a packed-bed chromatographic column to work in a continuous flow. Finally, we determined the three-dimensional (3D) models of this commercial lipase crystallized with and without phosphate at 2.0 and 1.7 Å resolutions, respectively.

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Synthesis of luminescent lanthanide complexes within crosslinked protein crystal matrices

Abstract: Eu(TTA)3phen was synthesized inside of crosslinked protein crystals. And we characterized the volumetric changes quantitatively induced by DMSO.

Cited by 9 publications

References 54 publications

Drug Sensing Protein Crystals Doped with Luminescent Lanthanide Complexes

Drug Sensing Protein Crystals Doped with Luminescent Lanthanide Complexes

Protein crystal based materials for nanoscale applications in medicine and biotechnology

Production of Cross-Linked Lipase Crystals at a Preparative Scale

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