Synthesis of Magnetic Chelator for High-Capacity Immobilized Metal Affinity Adsorption of Protein by Cerium Initiated Graft Polymerization

Abstract

A novel magnetic chelator with high adsorption capacity of protein by immobilized metal affinity adsorption was prepared by cerium (IV) initiated graft polymerization of tentacle-type polymer chains with iminodiacetic acid (IDA) chelating group on magnetic particles with hydroxyl groups. The micron-sized magnetic poly(vinyl acetate-divinylbenzene) (PVAc-DVB) particles were prepared by a modified suspension polymerization in the presence of oleic acid-coated magnetite nanoparticles and subsequently modified by ester exchange reaction to introduce functional hydroxyl groups. Bovine hemoglobin (BHb) was selected as a model protein to investigate the adsorption capacity of these magnetic particles. The magnetic particles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), superconducting quantum interference device (SQUID) magnetometry, diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), and X-ray diffraction (XRD). The results showed that the magnetic particles had an average size of 5 microm and superparamagnetism with saturation magnetization of 20.0 emu/g at room temperature. The protein adsorption indicated that the graft polymerization of tentacle type polymer chains on the magnetic particles could produce magnetic adsorbents with high adsorption capacity (1428.21 mg/g) and low nonspecific adsorption of protein. The magnetic particles with grafted tentacle polymer chains have potential application in large-scale affinity separation of proteins.

Novel open tubular CEC with tentacle‐type polymer stationary phase functionalized by phenylalanine

“…Polymer chains grafted onto the surface can exhibit excellent performance, such as high capacity for capturing proteins or other analytes and high activity for hydrolysis [15][16][17]. An epoxy-group containing a monomer, glycidyl methacrylate (GMA), is the commonly used precursor monomer.…”

“…Grafting GMA presents several advantages, such as easy grafting reaction, high reactivity of epoxy groups toward amino, carboxyl and hydroxyl end-groups, as well as the reduced environmental impact compared with other reactive monomers [18][19][20]. Therefore, GMA-based grafting reaction has been widely used for producing polymeric adsorbents on the base of membranes, films, chromatographic matrixes, fibers, and fabrics [15,19,[21][22][23][24]. However, no publication has reported the use of the method in CEC.…”

Surface modification of polyurethane via covalent immobilization of sugar-based trisiloxane surfactants

“…[11][12][13][14][15][16] Among these methods, photochemical treatment induced by UV irradiation is the most promising approach for its fast reaction rate, low cost of processing, simple equipment, and easy industrialization. [17] What's more, it is an effective way to directly immobilize various biocompatible moieties, such as poly(ethylene glycol), [18,19] poly(N-vinyl pyrrolidone), [20] heparin, [21,22] phospholipid polymer, [23][24][25] chitosan, [26][27][28] glycopolymers, [29] and hyaluronic acid [15] onto the surface of a substrate to obtain a target performance.…”

Novel negatively charged tentacle‐type polymer coating for on‐line preconcentration of proteins in CE

“…Both capillary inner surface area and ligand capacity were largely increased by coating the tentacle-type polymers, which can provide high analyte adsorption capacity. Though the tentacle-type polymermodified matrix has been widely applied for high capacity protein adsorption in HPLC [21][22][23], it was not used for protein extraction in CE. In the present study, a novel negatively charged tentacle-type polymer-modified capillary column was fabricated to characterize the performance of the tentacle-type polymer coating on the extraction and on-line preconcentration of proteins in CE.…”