Synthesis of merit-combined antimony tetroxide nanoflowers/reduced graphene oxide to synergistically boost real-time detection of nitric oxide released from living cells for high sensitivity

Cellular and Molecular Signatures of Oxidative Stress in Bronchial Epithelial Cell Models Injured by Cigarette Smoke Extract

“…More importantly, in situ sensors can be used for monitoring changes of markers of oxidative stress levels upon different stimuli and collecting kinetic information By making an appropriate choice of both the active material, for manufacturing the sensor, and the electrochemical technique used for the detection, it is possible to obtain sensors with high selectivity and sensitivity suitable for the detection of different markers of oxidative stress. In addition, recent works have also reported the possibility to use the electrochemical sensors direct in the culture plate for real-time monitoring of oxidative stress markers from living cells, without disturbing cell growth and without the need of medium sample collection for offline measurements [108,109]. In this way, the electrochemical sensor may have an easy-to-implement use in research laboratories, where the direct measurement of the markers of oxidative stress in cell supernatants can substitute current expensive and time-consuming biochemical and flow-cytometry-based approaches [110].…”

Research Progress of Graphene and Its Derivatives towards Exhaled Breath Analysis

“…Deng et al synthesized an antimony tetroxide (Sb 2 O 4 ) nanoflower/rGO nanocomposite material using a simple and environmentally friendly solvothermal method [ 57 ]. As shown in Figure 4 a, the combination of highly electroactive Sb 2 O 4 nanoflowers and rGO with a larger specific surface area shows a synergistic effect on the recognition of NO.…”

“… ( a ) Synthetic route of antimony tetroxide (Sb 2 O 4 ) nanoflower/rGO nanocomposite [ 57 ]. Copyright (2021), used with permission from Elsevier.…”

Sensing Nitric Oxide in Cells: Historical Technologies and Future Outlook

“…Electrochemical NO probes were among the first types of probes/sensors used to measure NO production, whether endogenously or exogenously released. One such example of an electrochemical NO probe to measure exogenous NO by Deng et al is composed of antimony tetroxide nanoflowers (Sb 2 O 4 ) and reduced graphene oxide (rGO) coated onto a glassy carbon electrode . In the culture medium of human umbilical vein endothelial cells (HUVECs) or adenocarcinomic human alveolar basal epithelial cells (A549), when induced with acetylcholine (ACh), there was a noticeable increase in sensor response, indicating increasing NO concentrations.…”

“…One such example of an electrochemical NO probe to measure exogenous NO by Deng et al is composed of antimony tetroxide nanoflowers (Sb 2 O 4 ) and reduced graphene oxide (rGO) coated onto a glassy carbon electrode. 41 In the culture medium of human umbilical vein endothelial cells (HUVECs) or adenocarcinomic human alveolar basal epithelial cells (A549), when induced with acetylcholine (ACh), there was a noticeable increase in sensor response, indicating increasing NO concentrations. Using a different approach, Kim et al developed a semiconductor surface comprised of porous ZnO NPs immobilized onto a Nafioncoated, poly(terthiophene-rGO) (poly(TTBA-rGO)) probe with a glassy carbon electrode base.…”