Synthesis of mesoporous organosilica nanoparticles with a high tetrasulphide content and large pores

Zhang, Yue; Wang, Yue; Song, Yaping; Luo, Jiangqi; Yan, Yu; Yu, Chengzhong

doi:10.1016/j.micromeso.2022.112316

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…MONs and MSNs were synthesised following a reported protocol, using CTAB and FC4 as surfactant templates in the alkaline aqueous system at 80 °C, BTES as the organosilica precursor, while TEOS and TBOS as the inorganic silica precursors [ 24 ]. In the reaction, both inorganic and organosilica precursors were hydrolyzed to provide Si-OH groups, which further assembled with the cationic head groups of the surfactant templates [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

“…After pDNA loading, PEI-MONs and PEI-MSNs were both negatively charged (−9.6 and −15.3 mV respectively), thus verifying successful pDNA loading. The higher surface charge of MON groups than MSN groups was presumably due to the increased organosilica content and thus decreased inorganic silica on the surface [ 24 ], also leading to slightly increased EGFP pDNA loading capacity.…”

Section: Resultsmentioning

confidence: 99%

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Mesoporous Organosilica Nanoparticles with Tetrasulphide Bond to Enhance Plasmid DNA Delivery

et al. 2023

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Cellular delivery of plasmid DNA (pDNA) specifically into dendritic cells (DCs) has provoked wide attention in various applications. However, delivery tools that achieve effective pDNA transfection in DCs are rare. Herein, we report that tetrasulphide bridged mesoporous organosilica nanoparticles (MONs) have enhanced pDNA transfection performance in DC cell lines compared to conventional mesoporous silica nanoparticles (MSNs). The mechanism of enhanced pDNA delivery efficacy is attributed to the glutathione (GSH) depletion capability of MONs. Reduction of initially high GSH levels in DCs further increases the mammalian target of rapamycin complex 1 (mTORc1) pathway activation, enhancing translation and protein expression. The mechanism was further validated by showing that the increased transfection efficiency was apparent in high GSH cell lines but not in low GSH ones. Our findings may provide a new design principle of nano delivery systems where the pDNA delivery to DCs is important.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Mesoporous Organosilica Nanoparticles with Tetrasulphide Bond to Enhance Plasmid DNA Delivery

et al. 2023

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Rational Design of Advanced Gene Delivery Carriers: Macrophage Phenotype Matters

Wang,

Yao,

Zhang

et al. 2024

Advanced Materials

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Nucleic acid delivery in hard‐to‐transfect macrophages have attracted increasing attention in diverse applications such as defence against bacterial infection. Regulated by microenvironments in specific applications, macrophages have a heterogenous nature and exist in different phenotypes with diverse functions, e.g., pro‐inflammatory and anti‐inflammatory. However, it is not clear whether macrophage phenotype affects nucleic acid delivery, and which one is harder to transfect, and the design of nucleic acid carriers in harder‐to‐transfect macrophage phenotypes is largely unexplored. Herein, it is first revealed that nucleic acid delivery efficacy in macrophages is influenced by phenotype: IL‐4‐treated “M2‐like” macrophages with suppressed mammalian target of rapamycin complex 1 (mTORC1) levels are harder‐to‐transfect than “M1‐like” macrophages for mRNA and DNA. This knowledge is then translated to the purpose‐design of gene delivery carriers for harder‐to‐transfect M2 phenotype macrophages dominant upon bacteria immune evasion. By loading chloroquine in tetrasulfide bond‐containing organosilica nanoparticles, the resultant composite promotes macrophage M2 polarization to M1 and increases mTORC1 levels for enhanced translation. The design is demonstrated in vitro and in vivo for pathogenic Escherichia coli (E. coli) and methicillin‐resistant Staphylococcus aureus (MRSA) infections. It is expected that the findings may provide new knowledge and gene delivery solutions in other applications where the M2 phenotype macrophage is dominant.

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Synthesis of wrinkled mesoporous silica encapsulated gold nanoparticles

Siddiki,

Poruri,

Balkus

2024

J Porous Mater

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Synthesis of mesoporous organosilica nanoparticles with a high tetrasulphide content and large pores

Cited by 3 publications

References 33 publications

Mesoporous Organosilica Nanoparticles with Tetrasulphide Bond to Enhance Plasmid DNA Delivery

Mesoporous Organosilica Nanoparticles with Tetrasulphide Bond to Enhance Plasmid DNA Delivery

Rational Design of Advanced Gene Delivery Carriers: Macrophage Phenotype Matters

Synthesis of wrinkled mesoporous silica encapsulated gold nanoparticles

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