Synthesis of molecular imprinted polymers for selective extraction of domperidone from human serum using high performance liquid chromatography with fluorescence detection

Salehi, Simin; Rasoul‐Amini, Sara; Adib, Noushin; Shekarchi, Mohammad

doi:10.1016/j.jchromb.2016.05.035

Cited by 13 publications

(2 citation statements)

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“…The MIP synthesis includes the formation of the complex between the monomer and the template molecule followed by template removal creating imprinting sites on the polymer surface [32]. Here, aminopropyl triethoxysilane was used as the monomer, tetraethoxysilane as crosslinker reagent, and phorbol 12-myristate 13-acetate was employed as a template in MIP synthesis for PE analysis.…”

Section: Mipcpesi Probe Characterizationmentioning

confidence: 99%

“…MIPs are materials that can provide a chemically selective surface for analyte enrichment. MIP synthesis generates imprinted sites, with similar size and shape, to a target analyte, which are prepared from cross-linking monomers and functional monomers in the presence of a target molecule, known as template [31,32]. MIPs have been employed in combination with many ambient ionization methods.…”

Section: Introductionmentioning

confidence: 99%

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Molecularly Imprinted Polymer-Coated Probe Electrospray Ionization Mass Spectrometry Determines Phorbol Esters and Deoxyphorbol Metabolites in Jatropha curcas Leaves

Silva

Carvalho

Vaz

et al. 2019

J. Am. Soc. Mass Spectrom.

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In this study, a molecularly imprinted polymer-coated probe electrospray ionization mass spectrometry (MIPCPESI-MS) method was developed for detection of phorbol esters (PEs) and deoxyphorbol metabolites in Jatropha curcas leaves. Such an approach was established by sticking on a metallic needle a molecularly imprinted polymer to particularly design a MIP-coated probe for selective sampling and ioni zation of PEs and deoxyphorbol metabolites. By a subsequent application of a high voltage and methanol, as spray solvent, ESI was generated for direct and rapid analysis under ambient and open-air conditions. MIP-coated probe exhibited a high sampling capacity of the PEs and its metabolites in methanolic extracts of J. curcas leaves compared with the non-imprinted polymer (NIP)-coated probe. MIPCPESI-MS allowed the detection of phorbol 12,13-diacetate (PDA) from J. curcas leaves with minimal sample preparation, and with detection limit and quantification reaching 0.28 μg/mL and 0.92 μg/mL, respectively. Also, good linearity was obtained with R 2 > 0.99 and precision and accuracy values between 4.06-13.49% and − 1.60 to − 15.26%, respectively. The current method was successfully applied to screening methanolic extracts of six different J. curcas leaf genotypes (three toxic and three non-toxic). PDA and three PE deoxyphorbol metabolites were identified only from toxic genotypes, in which PDA was determined with concentration ranging from 222.19 ± 23.55 to 528.23 ± 19.72 μg/g. All these findings support that the MIPCPESI-MS method developed here has a high potential for the analysis of PEs in plant extracts enabling differentiation of toxic and non-toxic genotypes earlier in the leaves.

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Section: Mipcpesi Probe Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%