Synthesis of monodisperse chitosan nanoparticles

Sullivan, David J.; Cruz-Romero, Malco C.; Collins, Timothy W.; Cummins, Enda; Kerry, Joseph P.; Morris, Michael A.

doi:10.1016/j.foodhyd.2018.05.010

Cited by 87 publications

(35 citation statements)

References 48 publications

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“…The zeta potential of FCN was + 34 mV, which meant that these nanoparticles could be stable for a long time. This is in agreement with the results of Sullivan et al (2018) who reported that when chitosan and TPP are mixed with each other, they spontaneously formed complexes with an overall positive surface charge. According to Yien, Zin, Sarwar, and Katas 2012, nanoparticles with a surface charge greater than + 30 mV are more stable.…”

Section: Characterization Of Fcnsupporting

confidence: 93%

Quality of postharvest strawberries: comparative effect of fungal chitosan gel, nanoparticles and gel enriched with edible nanoparticles coatings

Melo¹,

Pintado²,

Medeiros³

et al. 2020

IJFS

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This study compared, for the first time, the postharvest conservative action of edible fungal chitosan coatings (gel, nanoparticles and gel-nanoparticle) on the physico-chemical, sensorial and microbiological characteristics of strawberries. The nanoparticles were prepared by an ionic gelation method and characterized by dynamic light scattering and scanning electron microscopy. The antioxidant (DPPH* and ABTS*) activity of the edible coatings and the antimicrobial (macrodilution method) action against phytopathogenic fungi were verified. The nanoparticles had a size of 331.1 nm and a zeta potential of+ 34 mV. The gel, nanoparticles and gel+nanoparticles exhibited minimum inhibitory concentration values ranging from 4 to 5, 1.5 to 2.5 and 1.0 + 0.5 to 2.0 + 1.5 g.L-1, respectively. All the edible coatings exhibited antifungal action. All the coatings had high scavenging activity, especially the gel edible coating. The coatings, especially the gel+nanoparticles, decreased the weight loss, microbiological growth, soluble solids, maturity index and moisture loss of the strawberry and preserved the pH values, anthocyanin content, titratable acidity and sensory characteristics. Therefore, the use of chitosan edible coating containing nanoparticles can be a promising strategy to improve the post-harvest quality of strawberries.

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Section: Characterization Of Fcnsupporting

confidence: 93%

Quality of postharvest strawberries: comparative effect of fungal chitosan gel, nanoparticles and gel enriched with edible nanoparticles coatings

Melo¹,

Pintado²,

Medeiros³

et al. 2020

IJFS

View full text Add to dashboard Cite

show abstract

“…The molecular weight of the chitosan used was reported to affect the zeta potential as well. Low molecular weight chitosan had higher zeta potential compared to moderate molecular weight chitosan [18]. Binding and stability of CpG ODN on particles could not be shown in this study because of the negative band of 1, 2, 4 and 24 h incubation time (data not shown).…”

Section: Chitosan Was Synthesized and Characterized As Nanoparticlesmentioning

confidence: 63%

Preparation, characterization, and evaluation of chitosan-based nanoparticles as CpG ODN carriers

Iswanti

Nurulita²,

Djauzi

et al. 2019

Biotechnology & Biotechnological Equipment

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Chitosan-based particles are desirable materials for drug delivery because of their muco-adhesiveness in tissues, biocompatibility, low toxicity and effectiveness in antigen transport. It is still a challenge to prepare chitosan-based particles with high stability and by an effective method. Here we developed chitosan-based nanoparticles for the delivery of toll-like receptor 9 ligands, cytosine-phosphate-guanine oligodeoxynucleotides (CpG ODNs), which can induce the change from Th-2 type to Th-1 type immune response. Because of the low tolerance to DNase and less uptake into the cells of the free CpG ODNs, the development of positively charged carriers is necessary for the effective delivery of CpG ODNs into immune cells. Chitosan nanoparticles were prepared by ionic gelation, and the nanoparticles size and surface charge were measured by dynamic light spectroscopy and zeta potential analyzer, respectively. The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 lm, with a zeta potential of þ3.3 ± 0.4 mV. The toxicity of the chitosan nanoparticles to murine RAW264 macrophages was measured by formazan dye assay utilizing the water-soluble tetrazolium salt WST-8. Our chitosan nanoparticles exhibited no cytotoxicity to RAW264 cells. Finally, we evaluated the immunostimulatory activity of CpG ODNs loaded on chitosan nanoparticles using human peripheral blood mononuclear cells. CpG ODNs significantly enhanced the secretion level of interleukin-6 and interferon-c by cells compared to the free CpG ODNs. These results indicated that chitosan nanoparticles could be a good candidate for the delivery of CpG ODNs.

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“…Due the different pKa values 0.9, 1.9, 5.3, 7.7 and 9.5 ( [36,37], multiple anions (P3O10 5− , HP3O10 4− , H2P3O10 3− , H3P3O10 2− and H4P3O10 − ) may be present in solution depending on the pH when TPP is dissolved in water, which is undesirable as they can competitively react with the protonated ammonium groups (NH3 + ) of chitosan solution (pH 5.0). Therefore, the pH of TPP was adjusted to 5.0 to make sure that predominantly H2P3O10 3− (~67%) ions exist in solution; this is also beneficial in producing less polydisperse nanoparticles [38].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the mucoadhesive properties of chitosan nanoparticles prepared using different chitosan to tripolyphosphate (CS:TPP) ratios

Hejjaji

Smith

Morris

2018

International Journal of Biological Macromolecules

106

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 the interactions between CS:TPP nanoparticles and mucin were evaluated  the degree of interaction depends on the CS:TPP ratio, -potential and viscosity  a CS:TPP ratio of 4:1 displayed the strongest interaction with mucin  greater mucin binding efficiencies achieved at CS:TPP ratios of 4:1 and higher  a minimum CS:TPP ratio of 4:1 is required for stable interactions with mucin 3

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Synthesis of monodisperse chitosan nanoparticles

Cited by 87 publications

References 48 publications

Quality of postharvest strawberries: comparative effect of fungal chitosan gel, nanoparticles and gel enriched with edible nanoparticles coatings

Quality of postharvest strawberries: comparative effect of fungal chitosan gel, nanoparticles and gel enriched with edible nanoparticles coatings

Preparation, characterization, and evaluation of chitosan-based nanoparticles as CpG ODN carriers

Evaluation of the mucoadhesive properties of chitosan nanoparticles prepared using different chitosan to tripolyphosphate (CS:TPP) ratios

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