Synthesis of multifunctional bioresponsive polymers for the management of chronic wounds

Abstract

Novel multifunctional bioresponsive gelatin and alginate based hydrogels with in-built antioxidant regenerating system and antimicrobial properties were successfully synthesized. These hydrogels are based on the versatile reactions catalyzed by cellobiose dehydrogenase (CDH). CDH uses cellobiose and cello-oligosacharides as electron donors to reduce oxidized phenolic antioxidants, quinones, or molecular oxygen to H₂O₂ (a well-known antimicrobial agent). The antioxidant regenerating system consisting of CDH and cellobiose increased the ability of catechol to quench nitric oxide (NO), superoxide (O₂⁻) and hydroxyl radicals (OH•) in solution and when incorporated into hydrogels. The CDH loaded into the hydrogels free of oxidized phenolic antioxidants and quinones reduced molecular to H₂O₂ resulting in the complete inhibition of the growth of Stapylococcus aeureus, Bacillus subtilis, Pseudomonas putida, Escherichia coli and Cellulomonasmicrobium cellulans. This study therefore presents a new concept for synthesizing multifunctional bioresponsive chronic wound dressing polymers with in-built continuous antioxidant system able to continuously quench [reactive oxygen species (ROS) and reactive nitrogen species (RNOS)], and antimicrobial properties able to prevent microbial colonization of wound.

Broad-Spectrum Antimicrobial Polycarbonate Hydrogels with Fast Degradability

“…8 In the last decade, hydrogels have been studied and used as alternatives to gauze dressing materials in wound dressing applications. [9][10][11][12][13][14][15] Hydrogels have the ability 3 to maintain wound occlusion, creating a more conducive environment for tissue regeneration than exposed wounds. 16 4, 11, 17, 18 In addition, hydrogels offer a more versatile platform that allows for incorporation of growth factors to accelerate wound healing as compared to traditional gauze dressings.…”

Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production

“…from starch desizing), are added or generated by cellulolytic enzymes [10]. Similarly, the potential of CDH for medical application was recently demonstrated [21,22]. The main drawback of CDH for these applications is its relatively slow H 2 O 2 production rate compared to oxidases.…”

Biotechnological And Biomedical Applications Of Fungal Cellobiose Dehydrogenase

“…Dużą przeszkodą pojawiającą się podczas próby ich leczenia jest ciągłe uwalnianie proteaz, wolnych rodników, w tym reaktywnych form tlenu (ROS) i reaktywnych form azotu (RNOS) oraz duże ilości wysięków sprzyjających zakażeniom mikrobiologicznym. W ostatnich latach zespół profesora Nyanhongo opracował hydrożel na bazie CDH, który może zrewolucjonizować sektor opatrunków medycznych [65]. Skonstruowano go poprzez włączenie związku fenolowego -katecholu (pełniącego funkcję przeciwutleniacza) i celobiozy do hydrożelu (alginianu i/lub żelatyny), tworząc w ten sposób wielofunkcyjny polimer pomocny w leczeniu chronicznych ran.…”