Synthesis of multiply deuterated 3- and 21-monosulfates of allo-tetrahydrocorticosteroids as internal standards for mass spectrometry

A method for the determination and quantification of ketosteroid hormones in meat by mass spectrometry, based on the derivatization of the carbonyl moiety of steroids by O-methylhydroxylamine, is presented. The quantitative assay is performed by means of multiple-reaction-monitoring (MRM) scan mode and using the corresponding labelled species, obtained by reaction with d 3-methoxylamine, as internal standard. The accuracy of the method was established by evaluating artificially spiked samples, obtaining values in the range 90-110%. Recovery tests were performed on blank matrix samples spiked with non-natural steroids including trenbolone and melengestrol acetate. The latter experiment revealed that the yield of the extraction processes was approximately 60%. Good values of LOQ and LOD were achieved, making this method competitive with current hormone assay methods.

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Determination of ketosteroid hormones in meat by liquid chromatography tandem mass spectrometry and derivatization chemistry

Donna

Benabdelkamel

Taverna

et al. 2015

Anal Bioanal Chem

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Variations in GC–MS Response Between Analytes and Deuterated Analogs

2015

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Spontaneous conversion of prenyl halides to acids: application in metal-free preparation of deuterated compounds under mild conditions

et al. 2021

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Synthesis of multiply deuterated 3- and 21-monosulfates of allo-tetrahydrocorticosteroids as internal standards for mass spectrometry

Cited by 5 publications

References 14 publications

Determination of ketosteroid hormones in meat by liquid chromatography tandem mass spectrometry and derivatization chemistry

Determination of ketosteroid hormones in meat by liquid chromatography tandem mass spectrometry and derivatization chemistry

Variations in GC–MS Response Between Analytes and Deuterated Analogs

Spontaneous conversion of prenyl halides to acids: application in metal-free preparation of deuterated compounds under mild conditions

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