Synthesis of Nanobioconjugates with a Controlled Average Number of Biomolecules between 1 and 100 per Nanoparticle and Observation of Multivalency Dependent Interaction with Proteins and Cells

Saha, Arindam; Basiruddin, SK; Maity, Amit Ranjan; Jana, Nikhil R.

doi:10.1021/la402699a

Cited by 34 publications

(102 citation statements)

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“…Hydrophobic QDs are transformed into polyacrylate-coated hydrophilic QDs with an average of 100 primary amine groups per QD. 11 In this coating, four acrylates are used that include poly(ethylene glycol) methacrylate that provides the pegylated surface, N -(3-aminopropyl)-methacrylamide that introduces primary amine groups on the polymer shell, 3-sulfopropyl methacrylate that introduces SO 3 – groups, and bis[2-(methacryloyloxy)ethyl] phosphate that crosslinks the polymer shell. In addition, four acrylates are used in appropriate molar ratios to minimize particle–particle cross-linking.…”

Section: Resultsmentioning

confidence: 99%

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Supramolecular Host–Guest Chemistry-Based Folate/Riboflavin Functionalization and Cancer Cell Labeling of Nanoparticles

2017

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Nanoparticle-based cellular probes are commonly designed via covalent conjugation with affinity biomolecules. Those nanobioconjugates selectively interact with cell surface receptors and induce endocytosis followed by intracellular trafficking. However, this approach requires functional modification of biomolecules that may alter their biochemical activity. Here, we show that supramolecular host–guest chemistry can be utilized as an alternative approach in nanoparticle functionalization and selective cell labeling. We have used cyclodextrin-conjugated quantum dots (QDs) for supramolecular host–guest interaction-based functionalization with folate (QD-folate) and riboflavin (QD-riboflavin), where cyclodextrin acts as a host for the folate/riboflavin guest. We demonstrate that QD-folate and QD-riboflavin selectively label cells that have over-expressed folate/riboflavin receptors and induce the endocytosis pathway similar to covalently conjugated folate-/riboflavin-based nanoprobes. However, labeling is highly sensitive to the molar ratio of folate/riboflavin to cyclodextrin and incubation time. The presented functionalization/labeling approach is unique as it does not require covalent conjugation and may be extended for in vivo targeting application via simultaneous delivery of host and guest molecules.

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Section: Resultsmentioning

confidence: 99%

“… 7 − 10 Thus, research has been directed toward advanced and alternative approaches of functionalization. 11 − 15 …”

Section: Introductionmentioning

confidence: 99%

Supramolecular Host–Guest Chemistry-Based Folate/Riboflavin Functionalization and Cancer Cell Labeling of Nanoparticles

2017

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“…Nanobioconjugates are having a significant impact on the development of theranostic (therapeutic and diagnostic) tools in the biomedicine field [ 1 , 11 , 12 , 13 , 14 , 15 , 16 ] and have particular potential to revolutionize diagnostic approaches. Limitations of the nanobioconjugates are related to the uncontrolled number of affinity biomolecules that can be linked to the nanostructure, which leads to a variety of unwished phenomena [ 17 ]. For instance, a large number of biomolecules linked to nanostructures leads to hindered biochemical activity, alteration of the targeting and biomolecule properties and receptor cross-link [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Cajigas

Orozco

2020

Molecules

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Nanobioconjugates are hybrid materials that result from the coalescence of biomolecules and nanomaterials. They have emerged as a strategy to amplify the signal response in the biosensor field with the potential to enhance the sensitivity and detection limits of analytical assays. This critical review collects a myriad of strategies for the development of nanobioconjugates based on the conjugation of proteins, antibodies, carbohydrates, and DNA/RNA with noble metals, quantum dots, carbon- and magnetic-based nanomaterials, polymers, and complexes. It first discusses nanobioconjugates assembly and characterization to focus on the strategies to amplify a biorecognition event in biosensing, including molecular-, enzymatic-, and electroactive complex-based approaches. It provides some examples, current challenges, and future perspectives of nanobioconjugates for the amplification of signals in electrochemical biosensing.

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“…Less effort has been devoted to the development of a toolkit for chemical and biological catalysts working in tandem, since the general reaction conditions were often found to be contrary to normal conditions or were detrimental to enzyme stability [ 14 , 15 ]. This topic has great interest, since bioconjugated nanomaterials are finding increasing applications in biotechnology [ 16 , 17 , 18 ], as biosensors [ 19 , 20 , 21 ] and for drug delivery [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Stereoselective Double Reduction of 3-Methyl-2-cyclohexenone, by Use of Palladium and Platinum Nanoparticles, in Tandem with Alcohol Dehydrogenase

et al. 2018

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The combination of metal nanoparticles (Pd or Pt NPs) with NAD-dependent thermostable alcohol dehydrogenase (TADH) resulted in the one-flask catalytic double reduction of 3-methyl-2-cyclohexenone to 3-(1S,3S)-methylcyclohexanol. In this article, some assumptions about the interactions between a chemocatalyst and a biocatalyst have been proposed. It was demonstrated that the size of the NPs was the critical parameter for the mutual inhibition: the bigger the NPs, the more harmful for the enzyme they were, even if the NPs themselves were only moderately inactivated. Conversely, the smaller the NPs, the more minimal the TADH denaturation, although they were dramatically inhibited. Resuming, the chemocatalysts were very sensitive to deactivation, which was not related to the amount of enzyme used, while the inhibition of the biocatalyst can be strongly reduced by minimizing the NPs/TADH ratio used to catalyze the reaction. Among some methods to avoid direct binding of NPs with TADH, we found that using large Pd NPs and protecting their surfaces with a silica shell, the overall yield of 3-(1S,3S)-methylcyclohexanol was maximized (36%).

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Synthesis of Nanobioconjugates with a Controlled Average Number of Biomolecules between 1 and 100 per Nanoparticle and Observation of Multivalency Dependent Interaction with Proteins and Cells

Cited by 34 publications

References 55 publications

Supramolecular Host–Guest Chemistry-Based Folate/Riboflavin Functionalization and Cancer Cell Labeling of Nanoparticles

Supramolecular Host–Guest Chemistry-Based Folate/Riboflavin Functionalization and Cancer Cell Labeling of Nanoparticles

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Stereoselective Double Reduction of 3-Methyl-2-cyclohexenone, by Use of Palladium and Platinum Nanoparticles, in Tandem with Alcohol Dehydrogenase

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