Synthesis of nanomedicine hydrogel microcapsules by droplet microfluidic process and their pH and temperature dependent release

Liu, Ran; Wu, Qiong; Huang, Xing; Zhao, Xiaoting; Chen, Xinhua; Chen, Yonggang; Weitz, David A.; Song, Yujun

doi:10.1039/d1ra05207a

Cited by 20 publications

(8 citation statements)

References 78 publications

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“…Following oxygen plasma activation of both surfaces, PDMS microdevices were then attached to a glass slide and baked at 65 °C for 1 h. To make the channel surface hydrophobic, Aquapel (PGW autoglass LLC.) was used to functionalize the device through hydrophobic treatment …”

Section: Methodsmentioning

confidence: 99%

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Multichannel Multijunction Droplet Microfluidic Device to Synthesize Hydrogel Microcapsules with Different Core–Shell Structures and Adjustable Core Positions

Wu,

Huang,

Liu

et al. 2023

Langmuir

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Core−shell hydrogel microcapsules have sparked great interest due to their unique characteristics and prospective applications in the medical, pharmaceutical, and cosmetic fields. However, complex synthetic procedures and expensive costs have limited their practical application. Herein, we designed and prepared several multichannel and multijunctional droplet microfluidic devices based on soft lithography for the effective synthesis of core−shell hydrogel microcapsules for different purposes. Additionally, two different cross-linking processes (ultraviolet (UV) exposure and interfacial polymerization) were used to synthesize different types of core−shell structured hydrogel microcapsules. Hydrogel microcapsules with gelatin methacryloyl (GelMA) as the core and polyacrylamide (PAM) as the thin shell were synthesized using UV cross-linking. Using an interfacial polymerization process, another core−shell structured microcapsule with GelMA as the core and Ca 2+ cross-linked alginate with polyethylenimine (PEI) as the shell was constructed, and the core diameter and total droplet diameter were flexibly controlled by carving. Noteworthy, these hydrogel microcapsules exhibit stimuli-responsiveness and controlled release ability. Overall, a novel technique was developed to successfully synthesize various hydrogel microcapsules with core−shell microstructures. The hydrogel microcapsules possess a multilayered structure that facilitates the coassembly of cells and drugs, as well as the layered assembly of multiple drugs, to develop synergistic therapeutic regimens. These adaptable and controllable hydrogel microdroplets shall held great promise for multicell or multidrug administration as well as for high-throughput drug screening.

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Section: Methodsmentioning

confidence: 99%

“…was used to functionalize the device through hydrophobic treatment. 72 Synthesis of CdSe Nanocrystals. A 208 cm long microchannel constructed of a 153 cm stainless steel (SS) tube, a 40 cm PTFE tube, and a 15 cm poly(tetrafluoroethylene) (PTFE) tube was used to conduct the synthesis.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Multichannel Multijunction Droplet Microfluidic Device to Synthesize Hydrogel Microcapsules with Different Core–Shell Structures and Adjustable Core Positions

Wu,

Huang,

Liu

et al. 2023

Langmuir

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“…In recent studies, nanotechnology has been coupled with it and used to exert its effect. 22–24 Ginsenoside Rk1 is a rare ginsenoside monomer transformed from Ginsenoside Rg3, 25 and shows great potential efficacy against different types of cancer, including breast cancer, lung cancer and HCC. 26,27…”

Section: Introductionmentioning

confidence: 99%

“…In recent studies, nanotechnology has been coupled with it and used to exert its effect. [22][23][24] Ginsenoside Rk1 is a rare ginsenoside monomer transformed from Ginsenoside Rg3, 25 and shows great potential efficacy against different types of cancer, including breast cancer, lung cancer and HCC. 26,27 In the present study, we investigated for the first time whether ginsenoside Rk1 could exert good anti-cancer effects in HCC by regulating glutamine metabolism.…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rk1 regulates glutamine metabolism in hepatocellular carcinoma through inhibition of the ERK/c-Myc pathway

Yin

Liu

et al. 2022

Food Funct.

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“…For instance, Liu et al introduced hydrogel microcapsules, fabricated from natural polymer composites using microfluidics to produce nanomedicine. The microcapsules were capable of sustained release dependent on surrounding pH and temperature, suitable for oral administration [ 19 ]. One group of biomaterials widely investigated for drug delivery applications are natural polymers, including chitosan [ 20 ], hyaluronic acid [ 21 ], collagen [ 22 ] and alginate [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Bio-Printed Cardiac Patch for Sustained Delivery of Extracellular Vesicles from the Interface

Bar

Kryukov

Cohen

2022

Gels

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Cardiac tissue engineering has emerged as a promising strategy to treat infarcted cardiac tissues by replacing the injured region with an ex vivo fabricated functional cardiac patch. Nevertheless, integration of the transplanted patch with the host tissue is still a burden, limiting its clinical application. Here, a bi-functional, 3D bio-printed cardiac patch (CP) design is proposed, composed of a cell-laden compartment at its core and an extracellular vesicle (EV)-laden compartment at its shell for better integration of the CP with the host tissue. Alginate-based bioink solutions were developed for each compartment and characterized rheologically, examined for printability and their effect on residing cells or EVs. The resulting 3D bio-printed CP was examined for its mechanical stiffness, showing an elastic modulus between 4–5 kPa at day 1 post-printing, suitable for transplantation. Affinity binding of EVs to alginate sulfate (AlgS) was validated, exhibiting dissociation constant values similar to those of EVs with heparin. The incorporation of AlgS-EVs complexes within the shell bioink sustained EV release from the CP, with 88% cumulative release compared with 92% without AlgS by day 4. AlgS also prolonged the release profile by an additional 2 days, lasting 11 days overall. This CP design comprises great potential at promoting more efficient patch assimilation with the host.

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Synthesis of nanomedicine hydrogel microcapsules by droplet microfluidic process and their pH and temperature dependent release

Abstract: A kind of pH and temperature dependent interpenetrating hydrogel was designed and synthesized via crosslinking of alginate and polyethylene-glycol grafting chitosan by genipin for encapsulated nanomedicine with controlled release.

Cited by 20 publications

References 78 publications

Multichannel Multijunction Droplet Microfluidic Device to Synthesize Hydrogel Microcapsules with Different Core–Shell Structures and Adjustable Core Positions

Multichannel Multijunction Droplet Microfluidic Device to Synthesize Hydrogel Microcapsules with Different Core–Shell Structures and Adjustable Core Positions

Ginsenoside Rk1 regulates glutamine metabolism in hepatocellular carcinoma through inhibition of the ERK/c-Myc pathway

Three-Dimensional Bio-Printed Cardiac Patch for Sustained Delivery of Extracellular Vesicles from the Interface

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