Synthesis of Newcastle disease virus (NDV)-like envelopes in insect cells infected with a recombinant baculovirus expressing the haemagglutinin-neuraminidase of NDV

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“…Similar results were reported by Nagy et al (1990Nagy et al ( , 1991 showing expression of the HN gene of Hitchner B1, a lentogenic strain of NDV. However, in our case, NA activity was only detected after the recombinant HN from the extracellular fraction were concentrated by ultracentrifugation.…”

“…This finding was further strengthened when high levels of fluorescence signals were detected with IIF using NDV anti-sera polyclonal antibodies. Moreover, both HA and hemadsoprtion activities were inhibited by NDV anti-sera polyclonal antibodies indicating the biological functionality of the expressed protein which was also observed in the Baculovirus HN expression of parainfluenza type 3 virus (Coelingh et al, 1987) and NDV strain Hithner B1 (Nagy et al, 1991). In another study, serum samples from chickens immunised with the killed recHNAF2240 virus via the intra-mascular (i.m.)…”

Viruses and Virus-Like Particles in Biotechnology: Fundamentals and Applications

“…The latter have been shown to protect small laboratory animals as well as nonhuman primates against lethal challenge by Ebola and/or Marburg viruses [8]. HSV-VLP [40], NDV-VLP [49], Hantaan-VLP [13], and RVFV-VLP [62] exemplify other enveloped VLPs successfully expressed and assembled in the baculovirus/insect cell system. Substantial efforts were put forward to recreate the envelope of SARS coronavirus [63] and the envelope of viruses from Retroviridae family in a form that permits the efficient induction of broadly neutralizing antibodies.…”

Genetic engineering of bacteria and viruses

“…Although not tested for its suitability as a vaccine, VLPs based on the HN of NDV in a baculovirus system have been generated. These VLPs lack the NDV genome but present the HN on their surface as shown by HN specific monoclonal antibodies (Nagy et al, 1991b).…”