Synthesis of novel thiazoles bearing lupeol derivatives as potent anticancer and anti-inflammatory agents

Abstract

Lupeol is one of the most important metabolite in the class of terpenoids and possess excellent anticancer, anti-inflammatory, anti-diabetic activities etc. In the present study, the different thiazoles and oxazoles bearing lupeol derivatives were prepared to enhance their biological activity. Initially, the in vitro cytotoxic activity results showed that the synthesized lupeol derivatives (9a-9j and 10a-10e) showed significant activity against various cancer cells and the compounds 9h and 10b exhibited excellent activity against CAL27 cells. Further, these compounds 9h and 10b arrest the cell cycle at S phase and induce the late apoptosis in CAL27 cells by downregulating the BcL2 and vimentin expression and upregulating the Bax gene expression. Moreover, the lupeol derivatives showed dose-dependent anti-inflammatory activity by inhibiting the secretion of IL-6 cytokines in LPS-induced Raw 264.7 cells. Together, these results clearly indicated that the thiazoles and oxazoles bearing lupeol derivatives can used as chemotherapeutic drugs against cancer and inflammatory diseases.

Aloe juvenna Brandham & S.Carter as α‐Amylase Inhibitor and Hypoglycaemic Agent with Anti‐inflammatory Properties for Diabetes Management

“…Amorphous white powder, 1 H‐NMR (500 MHz, CDCl 3 ) δ: 4.68 (s, H‐29b), 4.56 (s, H‐29a), 3.20 (1H, dd , J =2.0, 6.0 Hz, H‐3), 1.67, 1.06, 0.96, 0.95, 0.83, 0.79, 0.76 (21 H, 7 s , 7‐CH 3 ). 13 CNMR (125 MHz, CDCl 3 ) δ: 38.19 (C‐1), 27.38 (C‐2), 79.1 (C‐3), 38.86 (C‐4), 55.31 (C‐5), 18.40 (C‐6), 34.41 (C‐7), 40.95 (C‐8), 50.57 (C‐9), 37.24 (C‐10), 21.05 (C‐11), 25.2 (C‐12), 38.91 (C‐13), 42.19 (C‐14), 27.52 (C‐15), 35.69 (C‐16), 43.09 (C‐17), 48.34 (C‐18), 48.08 (C‐19), 151.01 (C‐20), 29.75 (C‐21), 40.13 (C‐22), 28.0 (C‐23), 15.69 (C‐24), 15.74 (C‐25), 15.70 (C‐26), 14.20 (C‐27), 18.0 (C‐28), 109.38 (C‐29), 19.40 (C‐30) [47] …”

“…Using Nuclear Magnetic Resonance spectroscopy ( 1 H and 13 C NMR), they were characterized as lupeol (23) and vitexin ( 7) as compared to the data literature (Figure 2). [47,48] Both phytoconstituents were previously isolated from A. vera. [42,45] Anti-Diabetic Study…”

“…13 ). [47] Vitexin (7) Amorphous yellow powder, 1 H-NMR (500 MHz, DMSO-d 6 ,) δ: 13.5 (1H, 5-OH), 7.93 (2H, d, J = 8.6 Hz, H-2', 6'), 6.87 (2H, d, J = 9.0 Hz, H-3', 5'), 6.84 (1H, s, H-3), 6.5 (1H, s, H-6), 4.61 (1H, d, J = 9.5 Hz, H-1"). ).…”