Synthesis of oleic acid coated iron oxide nanoparticles and its role in anti-biofilm activity against clinical isolates of bacterial pathogens

Velusamy, P.; Su, Chia‐Hung; Shritama, A.; Kumar, Govindarajan Venkat; Jeyanthi, Venkadapathi; Pandian, K.

doi:10.1016/j.jtice.2015.07.018

Cited by 72 publications

(40 citation statements)

References 33 publications

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“…Functionalizing MNPs with OA resulted in an increase in negative potential to −29.2 mV [35,44]. To synthesize positive charge shifted MNPs, Arg (amino acid) induced a charge shift from −18 to −6 mV [45][46][47].…”

Section: Discussionmentioning

confidence: 99%

“…The effect of MNPs on preformed biofilm was previously described for OA-MNPs; however, its ability to inhibit new biofilm formation was not investigated [35]. Successful inhibition of biofilm development was reported using MNPs surface treated with polyvinylpyrrolidone and a thiourea derivative but this method included the use of an antibacterial agent [36].…”

Section: Mnps ( G/ml)mentioning

confidence: 99%

“…The higher suitability of S. aureus biofilm may be explained by its reported less negative charge than that of E. coli which may facilitate its interaction with the negatively charged OA-MNPs [35].…”

Section: Mnps ( G/ml)mentioning

confidence: 99%

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Effect of Surface Charge and Hydrophobicity Modulation on the Antibacterial and Antibiofilm Potential of Magnetic Iron Nanoparticles

Shebl

Farouk

Azzazy

2017

Journal of Nanomaterials

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Unmodified magnetic nanoparticles (MNPs) lack antibacterial potential. We investigated MNPs surface modifications that can impart antibacterial activity. Six MNPs species were prepared and characterized. Their antibacterial and antibiofilm potentials, surface affinity, and cytotoxicity were evaluated. Prepared MNPs were functionalized with citric acid, amine group, amino-propyl trimethoxy silane (APTMS), arginine, or oleic acid (OA) to give hydrophilic and hydrophobic MNPs with surface charge ranging from −30 to +30 mV. Prepared MNPs were spherical in shape with an average size of 6-15 nm. Hydrophobic (OA-MNPs) and positively charged MNPs (APTMS-MNPs) had significant concentration dependent antibacterial effect. OA-MNPs showed higher inhibitory potential against S. aureus and E. coli (80%) than APTMS-MNPs (70%). Both particles exhibited surface affinity to S. aureus and E. coli. Different concentrations of OA-MNPs decreased S. aureus and E. coli biofilm formation by 50-90%, while APTMS-MNPs reduced it by 30-90%, respectively. Up to 90% of preformed biofilms of S. aureus and E. coli were destroyed by OAMNPs and APTMS-MNPs. In conclusion, surface positivity and hydrophobicity enhance antibacterial and antibiofilm properties of MNPs.

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Section: Discussionmentioning

confidence: 99%

Section: Mnps ( G/ml)mentioning

confidence: 99%

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Effect of Surface Charge and Hydrophobicity Modulation on the Antibacterial and Antibiofilm Potential of Magnetic Iron Nanoparticles

Shebl

Farouk

Azzazy

2017

Journal of Nanomaterials

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“…Besides their specific magnetic properties [54], magnetite (Fe 3 O 4 ) nanoparticles are friendly to biological tissues and fluids, the biocompatibility [34,35,36,55] making them promising candidates for numerous biomedical applications, like: (1) drug delivery systems [56,57,58,59]; (2) hyperthermia cancer treatment [60,61,62,63,64]; (3) contrasting agent in magnetic resonance imaging [11,27,65,66]; (4) inhibition of biofilm development [67,68,69,70,71,72,73]. …”

Section: Discussionmentioning

confidence: 99%

Fabrication and Cytotoxicity of Gemcitabine-Functionalized Magnetite Nanoparticles

et al. 2017

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Nanotechnology has been successfully used for the fabrication of targeted anti-cancer drug carriers. This study aimed to obtain Fe3O4 nanoparticles functionalized with Gemcitabine to improve the cytotoxic effects of the chemotherapeutic substance on cancer cells. The (un) functionalized magnetite nanoparticles were synthesized using a modified co-precipitation method. The nanoconjugate characterization was performed by XRD, SEM, SAED and HRTEM; the functionalizing of magnetite with anti-tumor substances has been highlighted through TGA. The interaction with biologic media has been studied by means of stability and agglomeration tendency (using DLS and Zeta Potential); also, the release kinetics of the drug in culture media was evaluated. Cytotoxicity of free-Gemcitabine and the obtained nanoconjugate were evaluated on human BT 474 breast ductal carcinoma, HepG2 hepatocellular carcinoma and MG 63 osteosarcoma cells by MTS. In parallel, cellular morphology of these cells were examined through fluorescence microscopy and SEM. The localization of the nanoparticles related to the cells was studied using SEM, EDX and TEM. Hemolysis assay showed no damage of erythrocytes. Additionally, an in vivo biodistribution study was made for tracking where Fe3O4@Gemcitabine traveled in the body of mice. Our results showed that the transport of the drug improves the cytotoxic effects in comparison with the one produced by free Gemcitabine for the BT474 and HepG2 cells. The in vivo biodistribution test proved nanoparticle accumulation in the vital organs, with the exception of spleen, where black-brown deposits have been found. These results indicate that our Gemcitabine-functionalized nanoparticles are a promising targeted system for applications in cancer therapy.

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“…(reviewed in Gupta and Gupta, 2005; Sun et al, 2007; Li et al, 2010; Ianoş et al, 2012; Velusamy et al, 2016). Among all these methods, solution combustion synthesis was proved to be a promising alternative for the preparation of a considerable number of metal oxide nanopowders with multiple benefits: short preparation time, low energy consumption, cheap starting materials, self-sustained reaction, high effectiveness, simple procedure and low cost apparatuses and suitability for mass production (Ianoş et al, 2012; Huang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Colloidal Suspensions Based on Magnetic Iron Oxide Nanoparticles: Synthesis, Characterization and Toxicological Profile

et al. 2017

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The use of magnetic iron oxide nanoparticles in biomedicine has evolved intensely in the recent years due to the multiple applications of these nanomaterials, mainly in domains like cancer. The aim of the present study was: (i) to develop biocompatible colloidal suspensions based on magnetic iron oxide nanoparticles as future theranostic tools for skin pathology and (ii) to test their effects in vitro on human keratinocytes (HaCat cells) and in vivo by employing an animal model of acute dermal toxicity. Biocompatible colloidal suspensions were obtained by coating the magnetic iron oxide nanoparticles resulted during the solution combustion synthesis with a double layer of oleic acid, as innovative procedure in increasing bioavailability. The colloidal suspensions were characterized in terms of dynamic light scattering (DLS) and transmission electron microscopy (TEM). The in vitro effects of these suspensions were tested by means of Alamar blue assay and the noxious effects at skin level were measured using non-invasive methods. The in vitro results indicated a lack of toxicity on normal human cells induced by the iron oxide nanoparticles colloidal suspensions after an exposure of 24 h to different concentrations (5, 10, and 25 μg·mL−1). The dermal acute toxicity test showed that the topical applications of the colloidal suspensions on female and male SKH-1 hairless mice were not associated with significant changes in the quality of barrier skin function.

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Synthesis of oleic acid coated iron oxide nanoparticles and its role in anti-biofilm activity against clinical isolates of bacterial pathogens

Cited by 72 publications

References 33 publications

Effect of Surface Charge and Hydrophobicity Modulation on the Antibacterial and Antibiofilm Potential of Magnetic Iron Nanoparticles

Effect of Surface Charge and Hydrophobicity Modulation on the Antibacterial and Antibiofilm Potential of Magnetic Iron Nanoparticles

Fabrication and Cytotoxicity of Gemcitabine-Functionalized Magnetite Nanoparticles

Biocompatible Colloidal Suspensions Based on Magnetic Iron Oxide Nanoparticles: Synthesis, Characterization and Toxicological Profile

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