Synthesis of P(<scp>3HB</scp>‐<i>co</i>‐<scp>4HB</scp>) copolymer with target‐specific <scp>4HB</scp> molar fractions using combinations of carbon substrates

Iqbal, Nurhezreen Md; Amirul, Al-Ashraf Abdullah

doi:10.1002/jctb.4133

Cited by 14 publications

(13 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar observation was also reported by Chanprateep et al (2008), which stated that 1,4-butanediol is one of the effective carbon precursors for the increase in the 4HB molar fraction as compared to γ-butyrolactone. Biosynthesis of P(3HB-co-4HB) involves the conversion of 1,4butanediol to 4-hydroxybutyryl-CoA, before being converted to 4-hydroxybutyrate, where only a portion of 4-hydroxybutyrate will undergo a complex pathway to form 3-hydroxybutyrate (Steinbüchel & Lütke-Eversloh, 2003;Iqbal & Amirul, 2014;Norhafini et al, 2017). Hence, this further confirmed that the utilization of the optimum amount of 1,4-butanediol could greatly promote the production of 4HB monomer in copolymer P(3HB-co-4HB).…”

Section: Effect Of Different Ratio Of Carbon Precursors On Bacterial ...supporting

confidence: 58%

EFFECTS OF VARIOUS CARBON PRECURSORS COMBINATION IN REGULATING THE MOLAR FRACTION OF P(3HB-co-4HB) USING LOCALLY ISOLATED Cupriavidus sp. TMT11

Chai

Krishnan

Hang

et al. 2021

MABJ

View full text Add to dashboard Cite

The P(3HB-co-4HB) is commonly used for biomedical applications. This is due to the desired mechanical properties as well as biocompatibility, non-genotoxicity, and non-cytotoxicity of this copolymer. However, the production of P(3HB-co-4HB) with a specific 4HB molar fraction is still limited. In this study, carbon precursors at different combinations and concentration ratios have been used in the production of P(3HB-co-4HB) bioplastics using locally isolated Cupriavidus sp. TMT11. The carbon precursors 1,6-hexandiol, 1,4-butanediol, and g-butyrolactone were used to screen for high cell biomass, PHA content, and 4HB molar fraction through Gas Chromatography (GC) analysis. Generally, the combination of various carbon precursors showed an increase in cell biomass. The carbon combination of 1,4-butanediol and g-butyrolactone at the ratio of 4:2 and carbon combination of 1,6-hexanediol and g-butyrolactone at a ratio of 1:5 showed high amount of cell biomass above 0.35 g/L on day 3. Nevertheless, the 4HB molar fraction of both the combination was recorded as 9±0.27 mol% and 14±1.1 mol% respectively. The lowest amount of cell biomass and PHA yield were recorded with the carbon combination of 1,6-hexanediol and 1,4-butanediol with the ratio 5:1 at 0.55±0.13 g/L. However, the highest 4HB molar fraction of 89.37±3.6 mol% 4HB was recorded with this combination. The 4HB molar fraction above 80 mol% was reported with a carbon combination of 1,6-hexanediol +1,4-butanediol at 5:1, 1:1, and 2:4 ratio. The varying combination of carbon precursors biosynthesized a wide range of 4HB molar fractions ranging from 9.07 to 89.37 mol% 4HB.

show abstract

Section: Effect Of Different Ratio Of Carbon Precursors On Bacterial ...supporting

confidence: 58%

EFFECTS OF VARIOUS CARBON PRECURSORS COMBINATION IN REGULATING THE MOLAR FRACTION OF P(3HB-co-4HB) USING LOCALLY ISOLATED Cupriavidus sp. TMT11

Chai

Krishnan

Hang

et al. 2021

MABJ

View full text Add to dashboard Cite

show abstract

“…When grown on any of the saturated FAs, C. necator IBP/SFU-1 produced cell concentrations comparable to those achieved on oleic acid, but polymer concentrations were lower ( Figure 2 ): 65.9 and 64.2% on lauric and myristic acids, respectively, and only 49% on palmitic acid. Similarly lower parameters were obtained on saturated FAs compared to oleic acid in studies of C. necator USMAHM13 [ 63 ] and C. necator USMAA2-4 [ 62 ]. The most likely explanation lies in the physiological and biochemical properties of each strain and its ability to metabolize a certain fatty acid.…”

Section: Resultsmentioning

confidence: 72%

“…Those values were comparable to the corresponding parameters reported for C. necator B-10646 [ 60 ] and other strains including Alcaligenes sp. NCIM N5085 [ 61 , 62 ] grown on the same FA. Polymer concentration synthesized by C. necator IBP/SFU-1 was higher but cell concentration was lower than those reported for C. necator USMAHM13 [ 63 ].…”

Section: Resultsmentioning

confidence: 99%

“…The most likely explanation lies in the physiological and biochemical properties of each strain and its ability to metabolize a certain fatty acid. For the strain C. necator USMAA2-4, FAs, as effective substrates for bacterial cells, were ranked as follows: oleic acid > palmitic acid > myristic acid > lauric acid [ 62 ]. For C. necator USMAHM13, by contrast, cells grown on palmitic acid showed the lowest biomass concentration and PHA content [ 63 ].…”

Section: Resultsmentioning

confidence: 99%

“…That was comparable with the results obtained in the 72-h culture of the wild-type strain C. necator B-10646-7.1 g/L and 7–82.7%, respectively [ 64 ], and superior to the production parameters of another wild-type strain, C. necator H16-5.6 g/L and 77%, respectively [ 65 ]. Not all C. necator strains, however, are able to utilize palm oil, the wild-type strain C. necator USMAA2-4 failed to grow on palm oil [ 62 ].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources

et al. 2021

View full text Add to dashboard Cite

The bacterial strain isolated from soil was identified as Cupriavidus necator IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO2, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.

show abstract

Biotransformation of oleochemical industry by‐products to polyhydroxyalkanoate bioplastic using microbial fermentation: A review

Kakar

Amelia

Teng

et al. 2021

Environmental Quality Mgmt

View full text Add to dashboard Cite

For many decades, global efforts have been attempted to replace the petrochemical plastics with biodegradable and environment friendly plastics. The petrochemical plastics that require many years to decompose have profound consequences on the environment. A natural, biodegradable, and plastic‐like polymer, polyhydroxyalkanoate (PHA), has been extracted from and synthesized by microbes, particularly bacteria. However, the prospective large‐scale production of PHA faced an economic bottleneck for less production costs. Research has thus shifted to one of the most common alternatives, which is the utilization of less costly raw materials or carbon sources that include industrial by‐products and wastes, such as glycerine pitch and glycerol. This review elucidates the discovery, basic mechanisms, and applications of PHA, as well as provides insights regarding the importance of PHA conversion from glycerine pitch and glycerol. This review also summarizes some possible future modifications to improve the efficiency of glycerine pitch and glycerol as carbon sources in PHA production. The conversion endeavors of wastes to a valuable product, PHA, that not only reduces petrochemical plastic pollution, waste generation, and PHA production costs but also supports economic and resource sustainability, which ultimately further progress towards the cradle‐to‐cradle design and circular bioeconomy concept.

show abstract

Synthesis of P(3HB‐co‐4HB) copolymer with target‐specific 4HB molar fractions using combinations of carbon substrates

Cited by 14 publications

References 47 publications

EFFECTS OF VARIOUS CARBON PRECURSORS COMBINATION IN REGULATING THE MOLAR FRACTION OF P(3HB-co-4HB) USING LOCALLY ISOLATED Cupriavidus sp. TMT11

EFFECTS OF VARIOUS CARBON PRECURSORS COMBINATION IN REGULATING THE MOLAR FRACTION OF P(3HB-co-4HB) USING LOCALLY ISOLATED Cupriavidus sp. TMT11

Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources

Biotransformation of oleochemical industry by‐products to polyhydroxyalkanoate bioplastic using microbial fermentation: A review

Contact Info

Product

Resources

About