Synthesis of peptides on a resin by the mixed anhydride method

Solid‐Phase Peptide Synthesis

“…Semkin, Gafurova, and Shchukina (191) activated Boc amino acids with isobutylchloroformate and applied them to the solid-phase synthesis of Gly-Phe-Ala. I n a similar way, Tilak and Hollinden (231) coupled the mixed anhydride of Z-Ala and isobutylehloroformate to Phe-resin, and obtained a 97% yield of Ala-Phe after cleavage by saponification.…”

Detection and prevention of urethane acylation during solid phase peptide synthesis by anhydride methods

“…The yield of the DCC runs is based on the Val-resin, which is limiting; if based on the excess Bpoc-Gly the values would be of those shown. 6 After coupling, the peptides were cleaved from the resin with HF containing 10% anisóle at 0°for 1 hr. The crude mixture was extracted from the resin and fractionated on an ion-exchange column (0.9 X 60 cm, pH 3.49 citrate).…”