Synthesis of peptides onto the surface of poly(ethylene terephthalate) particle track membranes

Abstract

In this article, we describe the synthesis of polypeptides onto the whole specific surface of poly(ethylene terephthalate) particle track membranes. In this case, initial functionalization is necessary. It had been done by an optimized surface oxidation in accordance with the method of Marchant-Brynaert, J.; Deldime, M.; Dupont, I.; Dewez, J.-L.; Schneider, Y.-J. (J Colloid Interface Sci 1995, 173, 236). Subsequent activation with carbodiimide and reaction with multifunctional amines yielded an aminated surface. The synthesis of peptides was carried out by two different means: first, by the coupling of single amino acids analogous to the method of Merrifield, and second, by coupling of presynthesized polypeptides consisting of 18 helix-forming amino acids. Analytical characterization was carried out by fluorescence spectroscopy by using the label Fluram and confirmed by the results of X-ray photoelectron (XP) spectroscopy. Only the stepwise synthesis led to a dense surface functionalization with peptides, whereas the coupling of fragments resulted in lower yields of coupling.

Photoreactive Functionalization of Poly(ethylene terephthalate) Track‐Etched Pore Surfaces with “Smart” Polymer Systems

“…Oxidative hydrolysis (''carboxylation'') of PET membranes and subsequent reaction with tetraethylenepentamine (TEPA; ''amination'') were carried out according to Papra et al [25] The membranes were immersed in a reaction mixture of 10 g KMnO 4 in 200 mL H 2 SO 4 (0.75 N) at room temperature and shaken for 2.5 h. Membranes were then washed two times with water, treated four times for 2 min with HCl (6 N), washed four times with water and two times with ethanol and finally dried in an oven at 45 8C overnight.…”

“…[24] Consequently, such membranes have also already been used for ''grafting-from'' functionalizations. [14,15,17,19,25] Also, the resulting porous composite membranes may directly find interesting applications, for examples as enzyme-membrane reactor. [26,27] Furthermore, the knowledge about suited functionalization methods could be applied to the functionalization of other objects made from the same base polymer, for example components for ''labs-on-a-chip''.…”

“…[25,28] Terminally anchored poly(acrylic acid) (g-PAA) chains were then grafted on the membrane's pore surface and investigated in respect of the switching properties of the polymeric chains (ref. [29] ; Scheme 2).…”

Functionalized ensembles of nanoelectrodes as affinity biosensors for DNA hybridization detection

“…The number of À COOH groups present on the polymeric surface was increased by adapting the procedure proposed by Papra et al (1999). Briefly, NEEs were immersed in 0.32 M KMnO 4 , 0.75 N H 2 SO 4 at RT for different times (90 and 150 min), without shaking.…”

“…In order to further increase the detection performances demonstrated above, we tested the possibility of increasing the surface concentration of À COOH groups on the PC surface and, consequently, the amount of probe which can be bound, by treatment with KMNO 4 as described in earlier reports (Papra et al, 1999;Thorp et al, 1999). The number of generated carboxylic groups was again determined by fluorescence labeling using thionin acetate (THA).…”