2020
DOI: 10.1007/978-1-0716-0243-0_4
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Synthesis of PNA-Peptide Conjugates as Functional SNARE Protein Mimetics

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“…In order to identify orthogonal templates, an automated protocol for the synthesis of PNA strands was developed in collaboration with Denis Pahlke and Patrick Menzel that has recently found application in the synthesis of PNA-peptide conjugates as functional SNARE protein mimetics. [217] To this end, three PNA duplexes containing zero, two or three mismatched base pairs, as well as two duplexes of different length and sequence (10-mer, 12-mer) were synthesized. The stability of matched and mismatched pairs was studied by UV-Vis melting curve measurements.…”
Section: Discussionmentioning
confidence: 99%
“…In order to identify orthogonal templates, an automated protocol for the synthesis of PNA strands was developed in collaboration with Denis Pahlke and Patrick Menzel that has recently found application in the synthesis of PNA-peptide conjugates as functional SNARE protein mimetics. [217] To this end, three PNA duplexes containing zero, two or three mismatched base pairs, as well as two duplexes of different length and sequence (10-mer, 12-mer) were synthesized. The stability of matched and mismatched pairs was studied by UV-Vis melting curve measurements.…”
Section: Discussionmentioning
confidence: 99%