Synthesis of poly-3-hydroxybutyrate (PHB) by <i>Bacillus cereus</i> using grape residues as sole carbon source

Andler, Rodrigo; Pino, Valentina; Moya, Felipe; Soto, E. C.; Valdés, Cecilia T.; Andreeßen, Christina

doi:10.1080/24759651.2021.1882049

Cited by 22 publications

(4 citation statements)

References 55 publications

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“…Most of the sawdust utilizing bacterial isolates belonged to the Bacillus genus. Many reporters have described Bacillus species ideal for PHB production [30].…”

Section: Identification Of the Phb Producing Sd2 Isolate By 16sr Dna ...mentioning

confidence: 99%

Isolation and Characterization of the Bioplastic Producing Bacteria Using Low-Cost Substrate, Sawdust

Javaid,

Aslam,

Qaisar

et al. 2023

J. Engg. Res. & Sci.

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Plastics are routinely used in the packaging of materials as well as in industrial production. However, once in the environment, they are non-biodegradable, posing severe threats to the ecosystems. Bioplastic replaces conventional plastic, which is biodegradable due to its biological origin and does not affect environment. Sawdust is a very important agrowaste screened as a substrate for bioplastic production. In the present study, bacteria capable of producing bioplastic by utilizing sawdust as a cheap substrate were isolated and optimized for bioplastic production. Eight sawdust utilizing bacteria were isolated and the strains were designated as SD1 to SD8. These indigenous bacterial isolates were screened for bioplastic production using Sudan Black B Staining. Among the bacterial isolates, the bioplastics (PHB) production levels were 0.046 g/mL to 0.32 g/mL, while the maximum PHB (g/ml) production (0.32 ± 0.008g/mL) was given by SD2 isolate identified as the Bacillus cereus-SD2 strain through 16SrDNA sequencing. The isolate SD2 was optimized for bioplastic production at different growth conditions. The best temperature for the bioplastic production was 37˚C in the saw dust containing low-cost medium and yielded 0.32 ± 0.00 optical density at wavelength 235 nm of crotonic acid. The isolate SD2 showed a higher PHB (g/ml) yield of 0.31 ± 0.008 under alkaline conditions of pH 9. Sufficient oxygen was required for the higher PHB (g/ml) production by the bacterial isolate SD2, which yielded a 0.32 ± 0.006 level of PHB as compared to the non-aeration. The Bacillus cereus-SD2 is a promising bacterial which can produce environmentally friendly bioplastics using low-cost substrate. Finding more growth condition for enhanced bioplastics yields in future are suggested to scale up the production process at industrial level.

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“…Most of the sawdust utilizing bacterial isolates belonged to the Bacillus genus. Many reporters have described Bacillus species ideal for PHB production [30].…”

Section: Identification Of the Phb Producing Sd2 Isolate By 16sr Dna ...mentioning

confidence: 99%

Isolation and Characterization of the Bioplastic Producing Bacteria Using Low-Cost Substrate, Sawdust

Javaid,

Aslam,

Qaisar

et al. 2023

J. Engg. Res. & Sci.

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“…[ 22 ]. Halomonas strains have been found to produce PHA by utilizing a wide variety of carbohydrates under high saline conditions [ 23 ], whereas Ralstonia and Bacillus strains require unbalanced or unfavorable growth conditions [ 24 , 25 ]. Pseudomonas species have attracted attention for their ability to use a wide range of compounds as carbon sources for PHA production, including various industrial wastes and byproducts [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Production of Polyhydroxybutyrate by Genetically Modified Pseudomonas sp. phDV1: A Comparative Study of Utilizing Wine Industry Waste as a Carbon Source

Drakonaki,

Mathioudaki,

Geladas

et al. 2023

Microorganisms

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Pseudomonas sp. phDV1 is a polyhydroxyalkanoate (PHA) producer. The presence of the endogenous PHA depolymerase (phaZ) responsible for the degradation of the intracellular PHA is one of the main shortages in the bacterial production of PHA. Further, the production of PHA can be affected by the regulatory protein phaR, which is important in accumulating different PHA-associated proteins. PHA depolymerase phaZ and phaR knockout mutants of Pseudomonas sp. phDV1 were successfully constructed. We investigate the PHA production from 4.25 mM phenol and grape pomace of the mutants and the wild type. The production was screened by fluorescence microscopy, and the PHA production was quantified by HPLC chromatography. The PHA is composed of Polydroxybutyrate (PHB), as confirmed by 1H-nuclear magnetic resonance analysis. The wildtype strain produces approximately 280 μg PHB after 48 h in grape pomace, while the phaZ knockout mutant produces 310 μg PHB after 72 h in the presence of phenol per gram of cells, respectively. The ability of the phaZ mutant to synthesize high levels of PHB in the presence of monocyclic aromatic compounds may open the possibility of reducing the costs of industrial PHB production.

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“…These precursors are converted into acetyl-CoA and then to malonyl-coA, where specific CoA transferase (PhaG) forms (R)-3-hydroxyfatty acids from malonyl coA [ 34 , 35 ]. (R)-3-hydroxyfatty acids are accumulated in the form of mcl-PHA by Pha C [ 36 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

The Synthesis, Characterization and Applications of Polyhydroxyalkanoates (PHAs) and PHA-Based Nanoparticles

et al. 2021

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Polyhydroxyalkanoates (PHAs) are storage granules found in bacteria that are essentially hydroxy fatty acid polyesters. PHA molecules appear in variety of structures, and amongst all types of PHAs, polyhydroxybutyrate (PHB) is used in versatile fields as it is a biodegradable, biocompatible, and ecologically safe thermoplastic. The unique physicochemical characteristics of these PHAs have made them applicable in nanotechnology, tissue engineering, and other biomedical applications. In this review, the optimization, extraction, and characterization of PHAs are described. Their production and application in nanotechnology are also portrayed in this review, and the precise and various production methods of PHA-based nanoparticles, such as emulsion solvent diffusion, nanoprecipitation, and dialysis are discussed. The characterization techniques such as UV-Vis, FTIR, SEM, Zeta Potential, and XRD are also elaborated.

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Synthesis of poly-3-hydroxybutyrate (PHB) by Bacillus cereus using grape residues as sole carbon source

Cited by 22 publications

References 55 publications

Isolation and Characterization of the Bioplastic Producing Bacteria Using Low-Cost Substrate, Sawdust

Isolation and Characterization of the Bioplastic Producing Bacteria Using Low-Cost Substrate, Sawdust

Production of Polyhydroxybutyrate by Genetically Modified Pseudomonas sp. phDV1: A Comparative Study of Utilizing Wine Industry Waste as a Carbon Source

The Synthesis, Characterization and Applications of Polyhydroxyalkanoates (PHAs) and PHA-Based Nanoparticles

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