Synthesis of Polyacrylamide‐Based Redox Active Cryogel Using Click Chemistry and Investigation of Its Electrochemical Properties

Abstract

Ferrocene (Fc) functionalization of macroporous polyacrylamide‐based cryogel (PAAm‐Epx) is achieved by click chemistry. For this purpose, firstly, PAAm‐Epx cryogel with azide functionality (PAAm‐N3) is prepared by using acryalmide (AAm) as the monomer and allyl glycidyl ether (AGE) as the co‐monomer followed by subsequent ring opening reaction of epoxide ring of AGE in acidic medium. Azide groups of PAAm‐N3 is then modified with ethynyl ferrocene, leading to the redox active cryogel (PAAm−Fc) together with preserving the unique characteristic properties of the virgin PAAm‐Epx cryogel. Swelling, mechanical and morphological behaviours of PAAm‐Epx, PAAm‐N3 and PAAm−Fc are investigated. Detailed characterization of the PAAm−Fc is carried out by several techniques such as FTIR (Fourier Transform Infrared Spectroscopy), Scanning electron microscopy with energy dispersive X‐ray spectroscopy (SEM‐EDX), Inductively Coupled Plasma Optical Emmision Spectrometry (ICP‐OES, Cyclic (CV), and Differential Pulse Voltammetry (DPV). Electrochemical analysis confirms the electroactivity and the presence of chemically linked ferrocene unit to the cryogel.

The specific biopanning of single‐domain antibody against haptens based on a functionalized cryogel

“…Furthermore, the addition of certain chemicals during cryogel preparation could provide reactive groups for cryogels. In the case of polyacrylamide cryogels, for example, allyl glycidyl ether (AGE) is often used as a functional copolymer monomer for epoxy groups and as the basis for ligand immobilization 25,26 . These advantages and applications made cryogels to be used as a material for immobilizing ligands in biopanning.…”

The Specific Biopanning of Single-domain Antibody Against Haptens Based on a Functionalized Cryogel

“…In the case of polyacrylamide cryogels, for example, allyl glycidyl ether (AGE) was often used as a functional copolymer monomer for epoxy groups and as the basis for ligand immobilization. [25,26] These advantages and applications made cryogels to be used as a material for immobilizing ligands in biopanning.…”