Synthesis of Porous CoFe2O4 and Its Application as a Peroxidase Mimetic for Colorimetric Detection of H2O2 and Organic Pollutant Degradation

Abstract

Porous CoFe2O4 was prepared via a simple and controllable method to develop a low-cost, high-efficiency, and good-stability nanozyme. The morphology and microstructure of the obtained CoFe2O4 was investigated by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), high-resolution TEM (HRTEM), specific surface area and pore analysis, and Raman spectroscopy. The results show that the annealing temperature has an important effect on the crystallinity, grain size, and specific surface area of CoFe2O4. CoFe2O4 obtained at 300 °C (CF300) exhibits the largest surface area (up to 204.1 m2 g−1) and the smallest grain size. The peroxidase-like activity of CoFe2O4 was further verified based on the oxidation of peroxidase substrate 3,3’,5,5’-tetramethylbenzidine (TMB) in the presence of H2O2. The best peroxidase-like activity for CF300 should be ascribed to its largest surface area and smallest grain size. On this basis, an effective method of colorimetric detection H2O2 was established. In addition, the porous CoFe2O4 was also used for the catalytic oxidation of methylene blue (MB), indicating potential applications in pollutant removal and water treatment.

Synthesis, Catalytic Properties and Application in Biosensorics of Nanozymes and Electronanocatalysts: A Review

“…In another example, the catalytic steady-state kinetics for CoFe 2 O 4 CF300 was estimated using TMB and H 2 O 2 as substrates [71]. It was shown that the PO-like reaction followed a Michaelis-Menten kinetics toward both substrates.…”

“…The "co-interaction" between the His@AuNCs and RGO increases the speed of electron transfer from TMB to oxygen, leading to enhanced catalytic activity [41]. In another example, the catalytic steady-state kinetics for CoFe2O4 CF300 was estimated using TMB and H2O2 as substrates [71]. It was shown that the PO-like reaction followed a Michaelis-Menten kinetics toward both substrates.…”

“…Since then, the number of such materials has been growing constantly. These include different material types, such as Nafion-cytochrome c [ 85 ], supramolecular complexes of hydrogel [ 86 ], nanocomplexes of lanthanides [ 87 , 88 , 89 , 90 , 91 , 92 ], Co 3 O 4 @CeO 2 hybrid microspheres [ 93 ], transition metal oxides and their composites [ 71 , 94 , 95 , 96 , 97 , 98 , 99 ], as well as noble metals: Au [ 100 ], Fe/Au [ 101 ], Au/Pt [ 102 ], Pt [ 103 , 104 ], Ru [ 105 ], Pt/Ru [ 60 ], Pd, Pd@Pt [ 106 ], Pd/Pt [ 107 ], Pd@γ-Fe 2 O 3 [ 108 ], etc. In the last decade, special interest in bionanotechnology has focused on the use of NZs based on carbon materials such as fullerenes [ 109 ], Prussian blue [ 110 ], TiO 2 [ 111 ] or Fe 2 O 3 /Pt-modified [ 112 ] multi-walled carbon nanotubes, hemin-graphene hybrid nanosheets [ 113 ], Pt/Ru/3D graphene foam [ 114 ], graphene oxide [ 115 ], Au/Pt/Au-graphene oxide nanosheets [ 116 ], Pd-magnetic graphene nanosheets [ 117 ], hemin-graphene-Au hybrid [ 118 ], IrO 2 [ 119 ], Cu-Ag [ 120 ] or Fe 2 O 3 -modified graphene oxide [ 121 ] ( Figure 4 ), Co-modified magnetic carbon [ 122 ], Co 3 O 4 graphene composite [ ...…”

Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols

“…Artificial nanoenzymes have several advantages over natural enzymes, including high stability against denaturing and simple storage conditions [5,6]. Accordingly, many nanomaterials, including noble metal-based nanostructures, transition-metal dichalcogenides, carbon-based nanostructures, and metal-organic frameworks have been designed to have intrinsic enzyme-like activities, such as peroxidase [7,8], catalase [9,10], oxidase [11,12], lactase [13], and ferroxidase [14,15]. In addition, some nanomaterials with multiple enzyme-like activities have been developed, such as porous Co 3 O 4 nanoplates, which exert both peroxidase-and catalase-like activities [16].…”

Dual Enzyme-like Performances of PLGA Grafted Maghemite Nanocrystals and Their Synergistic Chemo/Chemodynamic Therapy for Human Lung Adenocarcinoma A549 Cells

“…Nanozyme is a kind of nanomaterial with natural mimic enzyme catalytic activity [2,3]. As a new generation of artificial enzyme, nanoenzyme has the advantages of simple synthesis [4], adjustable catalytic activity [5,6], high stability, low cost and easy operation [7,8]. They have become a promising alternative to natural enzymes and have attracted extensive exploration by biomedical researchers [9][10][11].…”