Synthesis of (RS)-abscisic acid

Roberts, Donald L.; Heckman, Robert A.; Hege, Bonita P.; Bellin, Stuart A.

doi:10.1021/jo01273a046

Cited by 90 publications

(28 citation statements)

References 4 publications

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“…These ground samples were mixed with 10 mL of freshly prepared 90% aqueous acetone (v/ v) containing 0.01% ascorbic acid (w / v). The internal standards 13C6-labeled IAA (Cohen et al, 1986) and 'H,-labeled ABA (Roberts et al, 1968) were added to each sample at 250 and 750 ng, respectively. The samples were stirred overnight at 4°C.…”

Section: Iaa and Aba Analysismentioning

confidence: 99%

“…Ion masses (m/z) associated with the 13C6-labeled IAA internal standard (136,195 m / z ) and the endogenous IAA (130,189 m / z ) were selectively monitored and quantified by isotope dilution techniques (Cohen et al, 1986). Natural ABA with ion masses selectively monitored at 162 and 190 m / z was quantified by isotope dilution using 'H,-labeled ABA (165, 193 m/z) as an internal standard (Roberts et al, 1968). Carrier flow was set at approximately 1 mL min-l (column pressure = 10 psi) using He as a carrier.…”

Section: Iaa and Aba Analysismentioning

confidence: 99%

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NaCI Reduces Indole-3-Acetic Acid Levels in the Roots of Tomato Plants Independent of Stress-Induced Abscisic Acid

Dunlap¹,

Binzel²

1996

Plant Physiology

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Indole-3-acetic acid (IAA) was measured in leaves and roots of tomato (Lycopersicon esculentum) genotypes subjected to salt stress. An abscisic acid (ABA)-deficient mutant of tomato (sitiens), the genetic parent (Rheinlands Ruhm, RR), and a commercial variety (Large Cherry Red, LCR) of tomato were treated with 50 to 300 mM NaCl in nutrient culture. Both LCR and RR had significantly higher levels of IAA in the roots compared with that in sitiens prior to treatment. The initial levels of IAA in the roots of LCR and RR declined by nearly 75% after exposure to NaCl, whereas those in roots from the sitiens mutant remained unchanged. IAA levels in the leaves of all genotypes remained unchanged or increased slightly in response to NaCl. ABA was highest in leaves from the normal genotypes after exposure to NaCl. ABA levels in the roots of sitiens were similar to the levels in the normal genotypes, whereas levels in the leaves were only 10% of the levels found in normal genotypes regardless of the salt treatment. Treatment of LCR and sitiens with exogenous ABA increased the ABA levels in leaves and roots, but there were no measurable changes in endogenous IAA. Therefore, the reduction in IAA appears to result from an ABA-independent effect of NaCl on IAA metabolism in the roots of stressed plants.

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Section: Iaa and Aba Analysismentioning

confidence: 99%

Section: Iaa and Aba Analysismentioning

confidence: 99%

NaCI Reduces Indole-3-Acetic Acid Levels in the Roots of Tomato Plants Independent of Stress-Induced Abscisic Acid

Dunlap¹,

Binzel²

1996

Plant Physiology

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“…While a number of authors have shown that the trans,trans analogue of ABA has much lower activity than ABA (1), only small differences were reported for growth inhibition of Lemna (14).…”

mentioning

confidence: 99%

Structure-Activity Correlations with Compounds Related to Abscisic Acid

Sondheimer¹,

Walton²

1970

Plant Physiol.

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“…The movement of ABA was studied with 2-l4C-abscisic acid produced according to the technique reported by Roberts et al (11). After treatment the explants were cut into various sections as indicated in Figure 3.…”

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confidence: 99%

Movement of (RS)-Abscisic Acid in the Cotton Explant

Ingersoll

Smith

1970

Plant Physiol.

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The movement of abscisic acid in the cotton explant was investigated by bioassay and radioisotope techniques. The rate of movement was 20 to 30 millimeters per hour with most of the abscisic acid moving unchanged through the explant into the basal agar. The rate of movement was the same through the abscission zone as through petiole tissue. Patterns of accumulation and metabolic products are discussed.Abscisic acid has been shown to be a very effective abscissionpromoting agent in the cotton explant bioassay (1,2,13,14). It has been presumed that abscisic acid is rapidly transported in the explant to the abscission zone, its site of action (1), but to date no direct proof of this phenomenon has been offered. An alternative possibility is that abscisic acid is immobile and that it induces the production of an abscission-promoting agent or stimulus which is transported. A study of the movement of ABA in the explant was undertaken to answer the questions (a) does ABA move from the site of application to the abscission zone, (b) what is the rate of movement, (c) does it accumulate in any area of the explant? MATERIAIS AND METHODS All experiments were conducted with cotton explants excised from 14-day-old seedlings of Gossypium hirsutwn L. Acala SJ-1 grown in vermiculite subirrigated with tap water. The seedlings were grown in a growth chamber at 32 i 3 C with a 16-hr photoperiod of 5.8 X 104 erg/cm2.sec provided by cool white fluorescent lamps supplemented by incandescent bulbs.Explants from the cotyledonary nodes were cut with hypocotyl segments 9 mm long and epicotyl and petiole stumps 5 mm long. The cotton explant bioassay method of Addicott et al. (1) was employed except where noted. Briefly, the bioassay involved placing 10 explants (each containing two abscission zones) in a covered Petri dish. The bases of the explants were pushed into a 5-mm layer of 1.5% agar. The cut surfaces of the epicotyl and petioles were then treated with 5-,ul droplets of agar (0.75%) delivered by a 500-,l hypodermic syringe. Abscission was tested for by applying a momentary force equivalent to 5 g to the distal end of the petiolar stumps. Each treatment represented two dishes of 10 explants, and the experiments were repeated three times.The movement of ABA was studied with 2-l4C-abscisic acid produced according to the technique reported by Roberts et al. (11). After treatment the explants were cut into various sections as indicated in Figure 3. The pooled tissue sections were soaked for 24 hr in sealed vials in 2 ml of 95% ethanol made to 0.05 N with acetic acid to leach out labeled compounds. Fifteen milliliters of scintillation fluid (100 g of napthalene; 5 g of PPO [2,5-diphenyloxazole]; 1 liter of 1,4-dioxane) were added to the ethanol solutions in each vial containing the tissue sections. The 14C activity was determined by counting for 20 min per sample in a Beckman liquid scintillation spectrometer model 100.Recovery of the '4C-labeled compounds from the tissue and the basal agar was done by extracting with 80% metha...

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Synthesis of (RS)-abscisic acid

Cited by 90 publications

References 4 publications

NaCI Reduces Indole-3-Acetic Acid Levels in the Roots of Tomato Plants Independent of Stress-Induced Abscisic Acid

NaCI Reduces Indole-3-Acetic Acid Levels in the Roots of Tomato Plants Independent of Stress-Induced Abscisic Acid

Structure-Activity Correlations with Compounds Related to Abscisic Acid

Movement of (RS)-Abscisic Acid in the Cotton Explant

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