Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria

Bruna, Nicolás; Collao, Bernardo; Tello, Alejandra; Caravantes, P.; Díaz-Silva, N.; Monrás, J. P.; Órdenes-Aenishanslins, Nicolás; Flores, Marcos; Espinoza-González, Rodrigo; Bravo, Daniela; Pérez-Donoso, José M.

doi:10.1038/s41598-018-38330-8

Cited by 56 publications

(47 citation statements)

References 64 publications

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“…[ 356 ] By preparing complementary oligonucleotides for the specific bacterial genes ( fnbA for S. aureus , mecA for MRSA, and wcaG for K. pneumoniae ) and labeling them for simultaneous detection, the detection limit of this method is as low as 10 2 colony forming units mL −1 . Although the CdTe QDs in this work were synthesized chemically, it is likely that CdTe QDs produced by yeasts and proteobacteria [ 336 ] may also be employed for the same purpose. Likewise, AuNPs and AgNPs have recently been conjugated with different viral DNA or antibodies to develop nanoprobes for detection of viruses such as the Ebola virus, severe acute respiratory syndrome (SARS) corona virus, Dengu virus, different types of hepatitis viruses, herpes simplex virus, human cytomegalovirus, Kaposi sarcoma‐associated herpesvirus, human immunodeficiency virus and influenza virus.…”

Section: Applicationsmentioning

confidence: 99%

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

et al. 2020

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Bacteria play an important role in the calcification process of natural minerals and within organisms ( Table 1). It is Microbe-mediated mineralization is ubiquitous in nature, involving bacteria, fungi, viruses, and algae. These mineralization processes comprise calcification, silicification, and iron mineralization. The mechanisms for mineral formation include extracellular and intracellular biomineralization. The mineral precipitating capability of microbes is often harnessed for green synthesis of metal nanoparticles, which are relatively less toxic compared with those synthesized through physical or chemical methods. Microbe-mediated mineralization has important applications ranging from pollutant removal and nonreactive carriers, to other industrial and biomedical applications. Herein, the different types of microbe-mediated biomineralization that occur in nature, their mechanisms, as well as their applications are elucidated to create a backdrop for future research.

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Section: Applicationsmentioning

confidence: 99%

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

et al. 2020

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“…One of the characteristics of biosynthesized QDs is the presence of biomolecules covering the nanostructure that in some cases determine their properties (Plaza et al, 2016; Ulloa et al, 2016; Bruna et al, 2019). The organic composition of CdS and CdS/CdSe QDs biosynthesized by our method was analyzed by FTIR spectroscopy (Figure 5).…”

Section: Resultsmentioning

confidence: 99%

“…Recently, the relevance of hydrogen sulfide (H 2 S) in the extracellular biosynthesis of CdS QDs by Antarctic bacteria (Gallardo et al, 2014; Plaza et al, 2016) and bacteria isolated from the Atacama desert (Bruna et al, 2019) has been described. A basic pH of the buffer (9.35) favors the deprotonation of H 2 S to generate S 2– in solution thus favoring the interaction with Cd 2+ to form nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, in order to develop simpler an economical protocols for production with lower environmental impact, the use of microorganisms to biosynthesize these nanoparticles has gained attention (Li et al, 2011; Hulkoti and Taranath, 2014). In addition, it has been reported that biosynthesized QDs display unique properties such as increased acid and salt stability (Ulloa et al, 2016, 2018; Bruna et al, 2019). Until now, the microorganism most used for QDs biosynthesis is Escherichia coli , capable of producing CdS, CdSe, and CdTe QDs (Sweeney et al, 2004; Park et al, 2010; Monras et al, 2012; Yan et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Biological Synthesis of CdS/CdSe Core/Shell Nanoparticles and Its Application in Quantum Dot Sensitized Solar Cells

Órdenes-Aenishanslins

Anziani‐Ostuni

Quezada

et al. 2019

Front. Microbiol.

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In the present work, we report the use of bacterial cells for the production of CdS/CdSe Core/Shell quantum dots (QDs), a complex nanostructure specially designed to improve their performance as photosensitizer in photovoltaic devices. The method requires the incorporation of L-cysteine, CdCl 2 and Na 2 SeO 3 to Escherichia coli cultures and allows a tight control of QDs properties. The obtained CdS/CdSe QDs were photophysically and structurally characterized. When compared to CdS QDs, the classical shift in the UV-visible spectra of Core/Shell nanostructures was observed in CdS/CdSe QDs. The nanosize, structure, and composition of Core/Shell QDs were confirmed by TEM and EDS analysis. QDs presented a size of approximately 12 nm (CdS) and 17 nm (CdS/CdSe) as determined by dynamic light scattering (DLS), whereas the fourier transform infrared (FTIR) spectra allowed to distinguish the presence of different biomolecules bound to both types of nanoparticles. An increased photostability was observed in CdS/CdSe nanoparticles when compared to CdS QDs. Finally, biosynthesized CdS/CdSe Core/Shell QDs were used as photosensitizers for quantum dots sensitized solar cells (QDSSCs) and their photovoltaic parameters determined. As expected, the efficiency of solar cells sensitized with biological CdS/CdSe QDs increased almost 2.5 times when compared to cells sensitized with CdS QDs. This work is the first report of biological synthesis of CdS/CdSe Core/Shell QDs using bacterial cells and represents a significant contribution to the development of green and low-cost photovoltaic technologies.

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“…The bacterial synthesized nanoparticles are either extracellular or intracellular depending on the type of organism and nanoparticles [3]. The biological synthesis gave a high control over the size distribution of nanoparticles and did not require the use of stabilizing agents and also the nanoparticles were highly stable for over 6 months [4,5].…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli-based synthesis of cadmium sulfide nanoparticles, characterization, antimicrobial and cytotoxicity studies

Shivashankarappa

Sanjay

2020

Braz J Microbiol

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The present research describes the synthesis of cadmium sulfide (CdS) nanoparticles from Escherichia coli under the influence of bacterial enzyme sulphate reductase and study on their cytotoxicity for applications in cancer therapy. Escherichia coli cells were used to synthesize CdS nanoparticles under different concentrations of cadmium chloride and sodium sulfide. The morphology of the nanoparticles was analysed using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) was used for elemental analysis of nanoparticles. Fourier-transform infrared spectroscopy analysis (FTIR) was performed to assess the functional groups of the nanoparticles. Crystalline nature of nanoparticles was assessed using powder X-ray diffraction (XRD). Antibacterial studies of CdS nanoparticles were carried out on foodborne pathogens and cytotoxicity studies were carried out on Mus musculus skin melanoma (B16F10) and human epidermoid carcinoma (A431) cell lines. CdS nanoparticle showed more cytotoxic effect on cancer cells compared with standard 5-aminolevulinic acid (5-ALA). The Escherichia coli-synthesized CdS nanoparticles showed highest zone of inhibition in the ratio 4:1 of cadmium chloride and sodium sulfide on all tested bacterial strains. The nanoparticles were also tested for haemolytic activity on RBC cells, which exhibited lower cytotoxicity than sodium dodecyl sulphate which was used as positive control. The cytotoxicity of CdS nanoparticles assessed on A431 cells showed an inhibition of 81.53% at 100 μM concentration while the cytotoxicity assessed on B16F10 cells showed an inhibition of 75.71% at 200 μM concentration which was much efficient than 5-ALA which showed an inhibition of 31.95% at a concentration against B16F10 cells and 33.45% against A431 cells at a concentration of 1 mM. Cadmium sulfide nanoparticles were thus found to be highly toxic on cancer cells compare with standard anticancerous drug 5-ALA.

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Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria

Cited by 56 publications

References 64 publications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Biological Synthesis of CdS/CdSe Core/Shell Nanoparticles and Its Application in Quantum Dot Sensitized Solar Cells

Escherichia coli-based synthesis of cadmium sulfide nanoparticles, characterization, antimicrobial and cytotoxicity studies

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