Synthesis of serine from glycine in mitochondrial fragments

The metabolism of glyoxylate by human- and rat-liver mitochondria

“…of mitochondrial total nitrogen could be isolated as formate after incubation for 1 hr., the corresponding value for the conversion of the C-1 atom of glyoxylate into carbon dioxide being 29 %. There was no evidence for the extensive conversion of [2-14C]glyoxylate into [14C]carbon dioxide, so that further attempts to identify the terminal metabolic products of the C-2 atom of glyoxylate in this system are needed; the demonstration of serine synthesis from glycine by mitochondrial fragments (Sanadi & Bennett, 1960) and by liver homogenates (Richert, Amberg & Wilson, 1962) is of interest in this connexion. SUMMARY 1.…”

Aspects of the metabolism of glycine and of porphyrins. The second Hopkins Memorial Lecture

“…It seems possible, however, that this type of argument is not valid. Sanadi & Bennett (1960) have recently described a particulate enzyme system from the mitochondrial fraction of chicken liver which brings about a net synthesis of serine from glycine, and this reaction does not require the addition of a folic acid derivative or of glyoxylic acid; moreover, added formaldehyde does not participate in serine formation in this system. It is conceivable that the reactions involved are those known already, i.e.…”