Synthesis of silica nanoparticles with controllable surface roughness for therapeutic protein delivery

Niu, Yuting; Yu, Meihua; Zhang, Jun; Yang, Yannan; Xu, Chun; Yeh, Michael W.; Taran, Elena; Gray, Peter P.; Yu, Chengzhong

doi:10.1039/c5tb01405k

Cited by 36 publications

(27 citation statements)

References 45 publications

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“…The western blot assay was further conducted to study knockdown efficiency of survivin protein in HCT116 cells (Figure ). The survivin protein expression level significantly decreased in the presence of KIT‐6‐MSNs‐C 18 +survivin siRNA (Figure a) and Oligofectamine+survivin siRNA (Figure b), compared to cells‐only group (Figure h).…”

Section: Resultsmentioning

confidence: 99%

Facile Synthesis of Large‐Pore Bicontinuous Cubic Mesoporous Silica Nanoparticles for Intracellular Gene Delivery

et al. 2016

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Mesoporous silica nanoparticles (MSNs) have promising biomedical applications; however the synthesis of MSNs with a bicontinuous cubic Ia3d mesostructure and large pore size has been a long‐standing challenge. Herein we report a systematic study on the synthesis of KIT‐6 type MSNs (KIT‐6‐MSNs) using fluorocarbon‐hydrocarbon mixed surfactants as templates and ethanol as a co‐solvent. KIT‐6‐MSNs have a pore size of 9 nm and a particle size in the range of 200–400 nm. The role of ethanol in the synthesis of KIT‐6‐MSNs is crucial because ethanol increases the miscibility of fluorocarbon and hydrocarbon surfactants, thereby increasing the packing parameter and favoring the formation of an Ia3d structure. Excessive fluorocarbon surfactants help the formation of nanosized morphology. After hydrophobic modification, the large‐pore KIT‐6‐MSNs show a high loading capacity towards genetic molecules and successfully deliver the survivin siRNA into a human colorectal carcinoma HCT116 cell line, knocking down the gene expression associated with cancer cell survival and proliferation.

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Section: Resultsmentioning

confidence: 99%

Facile Synthesis of Large‐Pore Bicontinuous Cubic Mesoporous Silica Nanoparticles for Intracellular Gene Delivery

et al. 2016

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“…The large‐pore dendritic silica shell after hydrophobic C18 modification is used to load a therapeutic mAb anti‐pAkt. After intracellular delivery, the doped fullerene in the core generates single oxygen ( 1 O 2 ) and fluorescence under UV light excitation, whereas anti‐pAkt provokes cell inhibition by blocking pAkt and downstream anti‐apoptotic protein Bcl‐2 . Compared to antibody treatment itself, the combination of PDT and antibody treatment significantly inhibits the cell growth by reducing the downstream anti‐apoptotic protein levels.…”

Section: Methodsmentioning

confidence: 99%

Core–Shell‐structured Dendritic Mesoporous Silica Nanoparticles for Combined Photodynamic Therapy and Antibody Delivery

Abbaraju

Yang

et al. 2017

Chemistry An Asian Journal

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Multifunctional core-shell-structured dendritic mesoporous silica nanoparticles with af ullerene-doped silica core, ad endritic silica shell and large pores have been prepared. The combination of photodynamic therapy and antibody therapeuticss ignificantly inhibits the cancer cell growth by effectively reducing the level of anti-apoptotic proteins.In the past decades, with the rapid development of nanotechnology,av ariety of nanoparticles such as polyesters and polyacrylamide, [1] liposomes, [2][3][4] dendrimers, [5,6] magnetic [7,8] and other inorganic nanoparticles [9][10][11] have been widely used as carriers for photodynamict herapy (PDT). The combinationo f chemo-and photodynamic therapy [12] is an effective approach for many cancer treatments with furthere nhanced therapeutic efficacy. [13][14][15] However,c onventional chemotherapy using small-molecular-weight anticancer drugs has caused drug resistancea nd this problem exists in combinational therapy. [16] One promising alternative to chemotherapy for cancer treatment is antibody therapy.T he combined PDT and antibody delivery has been discovered as ap romising strategy for cancer treatment by conjugating photosensitizers with monoclonal antibodies (mAbs). [17] However,t here are some intrinsic issues, such as technical difficulty in chemical coupling and reduced phototoxicity. [18][19][20][21][22] The design of suitable carriers holds promise for improved combination therapy of photosensitizers and protein/antibody therapeutics by addressing these problems.Recently,m esoporous silica nanoparticles( MSNs) have attracted attention mainly due to their easy functionalization, large surface area and high pore volumef or high payload of therapeutic agents and effective cellular delivery. [23,24] Monodispersed MSNs with small pores have applications limited to small-drug delivery. [25] The discovery of dendritic mesoporous silica nanoparticles (DMSNs) with large and open pore channels makes them attractive candidates for delivering large biomolecules. [26,27] Lin et al. reported the advantage of magnetic DMSNsf or doxorubicin hydrochloride (DOX) delivery. [28] Later, Zhao and co-workersr eported the synthesis of 3D-DMSN grown on gold and silver nanoparticles. [29] The composite DMSN fabricated so far have been used for real-time fluorescence imaging, magnetic resonance imaging, [30] adsorption [31] and protein delivery. [29] However,tothe best of our knowledge, there is no report using DMSNs as am ultifunctional delivery platform for both photosensitizers and mAbs.Herein, we report the synthesis of multifunctional core-shell structured nanoparticles consisting of as olid silica-fullerene core and ad endritic silica shell with large pores (Scheme 1). The silica core doped with fullerene( C 60 )a cts as ap hotosensitizer and fluorescent agentsf or imaging. The large-pore dendritic silica shell after hydrophobic C18 modification is used to load at herapeutic mAb anti-pAkt. After intracellular delivery, the doped fullerene in the core generates single ox...

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Section: Silica Nanoparticlesmentioning

confidence: 99%

“…[115] The AuNPs were connected by disulfide linkage, which could be cleaved by intracellular disulfide-reducing antioxidant and subsequent resulted in the release of the luciferase and luciferin. [124,136,137] Using the chemical modification method to connect the proteins with the silica nanoparticles is another powerful way to deliver the proteins to the target site. Recently, Li et al prepared the SiNPs with ultrasmall luminescent crystals embedding for BSA protein delivery.…”

Section: Silica Nanoparticlesmentioning

confidence: 99%

Rational Design of Nanocarriers for Intracellular Protein Delivery

et al. 2019

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Protein/antibody therapeutics have exhibited the advantages of high specificity and activity even at an extremely low concentration compared to small molecule drugs. However, they are accompanied by unfavorable physicochemical properties such as fragile tertiary structure, large molecular size, and poor penetration of the membrane, and thus the clinical use of protein drugs is hindered by inefficient delivery of proteins into the host cells. To overcome the challenges associated with protein therapeutics and enhance their biopharmaceutical applications, various protein‐loaded nanocarriers with desired functions, such as lipid nanocapsules, polymeric nanoparticles, inorganic nanoparticles, and peptides, are developed. In this review, the different strategies for intracellular delivery of proteins are comprehensively summarized. Their designed routes, mechanisms of action, and potential therapeutics in live cells or in vivo are discussed in detail. Furthermore, the perspective on the new generation of delivery systems toward the emerging area of protein‐based therapeutics is presented as well.

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Synthesis of silica nanoparticles with controllable surface roughness for therapeutic protein delivery

Cited by 36 publications

References 45 publications

Facile Synthesis of Large‐Pore Bicontinuous Cubic Mesoporous Silica Nanoparticles for Intracellular Gene Delivery

Facile Synthesis of Large‐Pore Bicontinuous Cubic Mesoporous Silica Nanoparticles for Intracellular Gene Delivery

Core–Shell‐structured Dendritic Mesoporous Silica Nanoparticles for Combined Photodynamic Therapy and Antibody Delivery

Rational Design of Nanocarriers for Intracellular Protein Delivery

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