Synthesis of Silver and Gold Nanoparticles: Chemical and Green Synthesis Method and Its Toxicity Evaluation against Pathogenic Bacteria Using the ToxTrak Test

Tyagi, Pankaj; Quispe, Cristina; Herrera‐Bravo, Jesús; Tyagi, Shruti; Barbhai, Mrunal Deepak; Kumar, Manoj; Dablool, Anas S.; Alghamdi, Saad; Batiha, Gaber El‐Saber; Sharifi‐Rad, Javad; Ramniwas, Seema

doi:10.1155/2021/3773943

Cited by 24 publications

(5 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same group has also reported the use of Duchesnea indica for the development of Zn-doped CuO NPs (Zn/CuO NPs) exhibiting a spherical morphology. The green synthesized Zn/CuO NP's anticancer ability was widely studied using A-498 cancer cells and normal human epithelial cells at different concentrations (10,20,40,80, and 160 µg/mL). Figure 11 presents the microscopic images of dead cells on tumor cells treated with green-synthesized Zn/CuO NPs with (hematoxylin-eosin) H&E and (Fluorescein isothiocyanate) FITC staining.…”

Section: Results Of Toxicity and Biocompatibility Studies For Green S...mentioning

confidence: 99%

See 1 more Smart Citation

Green Synthesis of Metal and Metal Oxide Nanoparticles: A Review of the Principles and Biomedical Applications

Radulescu,

Surdu,

Ficai

et al. 2023

IJMS

View full text Add to dashboard Cite

In recent years, interest in nanotechnology has increased exponentially due to enhanced progress and technological innovation. In tissue engineering, the development of metallic nanoparticles has been amplified, especially due to their antibacterial properties. Another important characteristic of metal NPs is that they enable high control over the features of the developed scaffolds (optimizing their mechanical strength and offering the controlled release of bioactive agents). Currently, the main concern related to the method of synthesis of metal oxide NPs is the environmental impact. The physical and chemical synthesis uses toxic agents that could generate hazards or exert carcinogenicity/environmental toxicity. Therefore, a greener, cleaner, and more reliable approach is needed. Green synthetic has come as a solution to counter the aforementioned limitations. Nowadays, green synthesis is preferred because it leads to the prevention/minimization of waste, the reduction of derivatives/pollution, and the use of non-toxic (safer) solvents. This method not only uses biomass sources as reducing agents for metal salts. The biomolecules also cover the synthesized NPs or act as in situ capping and reducing agents. Further, their involvement in the formation process reduces toxicity, prevents nanoparticle agglomeration, and improves the antimicrobial activity of the nanomaterial, leading to a possible synergistic effect. This study aims to provide a comprehensive review of the green synthesis of metal and metal oxide nanoparticles, from the synthesis routes, selected solvents, and parameters to their latest application in the biomedical field.

show abstract

Section: Results Of Toxicity and Biocompatibility Studies For Green S...mentioning

confidence: 99%

“…In the field of tissue engineering, the development of metallic and metal oxide NPs has been amplified, especially for their antibacterial properties [9,10].…”

Section: Overview Of the Use Of Nanoparticles In Tissue Engineeringmentioning

confidence: 99%

Green Synthesis of Metal and Metal Oxide Nanoparticles: A Review of the Principles and Biomedical Applications

Radulescu,

Surdu,

Ficai

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…The NP dosage considers both the actual quantity of NPs that are absorbed by a single cell and the initial concentration of NPs in the cells. For in-vitro and in-vivo experimental models, it is crucial to define genuine and pertinent dosage regimes for risk evaluation in public health [68], [69]. By all means, nanotechnologists should study the toxicity of NPs based on actual dosages rather than inflated ones to elicit a biological reaction.…”

Section: Possible Side Effects Of Copper Nanoparticlesmentioning

confidence: 99%

Development of copper nanoparticles and their prospective uses as antioxidants, antimicrobials, anticancer agents in the pharmaceutical sector

Tyagi

Arya

Mazumder

et al. 2023

Precision Nanomedicine

View full text Add to dashboard Cite

In this study, we review the synthesis of copper nanoparticles (CuNPs) and their prospective uses as antioxidants, antimicrobials, and anticancer agents in the pharmaceutical sector. Copper nanoparticles could be created through a variety of methods, including chemical reduction, green synthesis, physical methods, electrochemical deposition, and the microemulsion method. These methods make it possible to precisely create nanoparticles with the necessary shapes, sizes, and surface properties, which in turn affect how well they perform biologically. CuNPs have strong antioxidant properties because they can scavenge reactive oxygen species (ROS) and prevent oxidative damage. In addition to their antioxidant properties, CuNPs show antibacterial activity against a number of microbes, including bacteria, fungi, and viruses. The potential of CuNPs as an anticancer agent has also been extensively investigated. These nanoparticles have toxic effects on a variety of cancer cell lines by inducing apoptosis, inhibiting cell proliferation, and preventing tumour angiogenesis. Because of their specific toxicity towards cancer cells while protecting normal cells, they provide the fascinating potential for tailored therapy to overcome multidrug resistance. CuNPs have also been studied in relation to their usage in medications. They may be utilised to improve drug delivery systems, wound healing, diagnostic imaging, immunotherapy, and anti-inflammatory and antioxidant effects. They can also be used to improve pharmaceutical stability, bioavailability, and provide controlled release. More research is needed to ensure their safety, enhance their synthesis processes, and explore their full potential in therapeutic situations

show abstract

“…The fundamental properties, such as electronic, optical, magnetic, and catalytic properties, are controlled by the size and shape of the nanoparticle. The level of uniformity or dispersity in biologically produced nanoparticles can vary based on the specific biological method, the involved organisms or biological materials, and the process conditions [62,63]. A few studies also reported that biologically synthesized AuNPs might be a suitable alternative in comparison to chemically synthesized AuNPs for coating antimicrobial medicines in the pharmaceutical industry.…”

Section: Antifungal Activity Of Aunps By Agar Well Assay and Their Sc...mentioning

confidence: 99%

Biological Synthesis, Characterization, and Therapeutic Potential of S. commune-Mediated Gold Nanoparticles

Alqurashi,

Almalki,

Ibrahim

et al. 2023

Biomolecules

View full text Add to dashboard Cite

Green-synthesized gold nanoparticles demonstrate several therapeutic benefits due to their safety, non-toxicity, accessibility, and ecological acceptance. In our study, gold nanoparticles (AuNPs) were created using an extracellular extract from the fungus Schizophyllum commune (S. commune). The reaction color was observed to be a reddish pink after a 24 h reaction, demonstrating the synthesis of the nanoparticles. The myco-produced nanoparticles were investigated using transmission electron microscopy (TEM), dynamic light scattering (DLS), and UV–visible spectroscopy. The TEM pictures depicted sphere-like shapes with sizes ranging from 60 and 120 nm, with an average diameter of 90 nm, which is in agreement with the DLS results. Furthermore, the efficiency of the AuNPs’ antifungal and cytotoxic properties, as well as their production of intracellular ROS, was evaluated. Our findings showed that the AuNPs have strong antifungal effects against Trichoderma sp. and Aspergillus flavus at increasing doses. Additionally, the AuNPs established a dose-dependent activity against human alveolar basal epithelial cells with adenocarcinoma (A549), demonstrating the potency of synthesized AuNPs as a cytotoxic agent. After 4 h of incubation with AuNPs, a significant increase in intracellular ROS was observed in cancer cells. Therefore, these metallic AuNPs produced by fungus (S. commune) can be used as an effective antifungal, anticancer, and non-toxic immunomodulatory delivery agent.

show abstract

Synthesis of Silver and Gold Nanoparticles: Chemical and Green Synthesis Method and Its Toxicity Evaluation against Pathogenic Bacteria Using the ToxTrak Test

Cited by 24 publications

References 65 publications

Green Synthesis of Metal and Metal Oxide Nanoparticles: A Review of the Principles and Biomedical Applications

Green Synthesis of Metal and Metal Oxide Nanoparticles: A Review of the Principles and Biomedical Applications

Development of copper nanoparticles and their prospective uses as antioxidants, antimicrobials, anticancer agents in the pharmaceutical sector

Biological Synthesis, Characterization, and Therapeutic Potential of S. commune-Mediated Gold Nanoparticles

Contact Info

Product

Resources

About