Synthesis of silver nanoparticles from Phenerochaete chrysosporium (MTCC-787) and their antibacterial activity against human pathogenic bacteria

Saravanan, Muthupandian; Arokiyaraj, Selvaraj; Thangavelu, Lakshmi; Pugazhendhi, Arivalagan

doi:10.1016/j.micpath.2018.02.008

Cited by 229 publications

(95 citation statements)

References 34 publications

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“…[1][2][3] Among the antibiotic nanomaterials, silver nanoparticles (AgNPs) were endowed to more different fields in our daily life, including textiles, sanitary articles, and cosmetics, because of their excellent broad-spectrum antimicrobial properties. [3][4][5][6][7][8][9] There have been many biomedical products containing AgNPs on the market to date, such as wound dressings, catheters, bone cement, and artificial cardiac valves. 10,11 With the growing usage of nanosilver-containing products, people are more likely to be exposed to AgNPs.…”

Section: Introductionmentioning

confidence: 99%

<p>Size-dependent cellular uptake and localization profiles of silver nanoparticles</p>

Guo

Liu

et al. 2019

IJN

222

118

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Purpose: Silver nanoparticles (AgNPs) have been widely applied in various fields as excellent antibacterial reagents over the past decades. Although the particle size is considered as the most crucial factor influencing cellular uptake, transportation, and accumulation behaviors, there are still many controversies regarding the correlation between size and uptake of AgNPs. In this study, size-dependent cellular uptake of AgNPs with different diameters was investigated in B16 cells. Methods: The uptake of AgNPs was investigated by inductively coupled plasma-mass spectrometry (ICP-MS) and transmission electron microscopic (TEM) imaging in B16 cells. Results: Twenty nanometer and 100 nm AgNPs had the lowest and highest uptake efficiency at both 12 hours and 24 hours, respectively. Smaller AgNPs crossed the plasma membrane faster with uniform distribution: 5 nm AgNPs were detected in both cytoplasm and nucleus at 0.5 hours after incubation. Larger AgNPs were extremely difficult to migrate: 100 nm AgNPs were detected in the nucleus at 12 hours after incubation. Internalization of AgNPs was directly observed, mainly within membrane-bound structures, such as intracellular vesicles and late endosomes. The uptake of all four-sized AgNPs (5 nm, 20 nm, 50 nm, 100 nm) decreased significantly after the pre-treatment with chlorpromazine hydrochloride, which can specifically inhibit the clathrin-mediated endocytosis. The internalization efficiencies of AgNPs (5 nm, 20 nm, 50 nm) were markedly reduced by methyl-β-cyclodextrin, a specific caveolin-mediated endocytosis inhibitor, whereas 5-(N-ethyl-N-isopropyl) amiloride as an inhibitor of macropinocytosis inhibited the uptake of larger sizes of AgNPs (50 nm and 100 nm). Conclusion: The results suggest that the size of AgNPs can not only affect the efficiency of cellular uptake, but also the type of endocytosis. The clathrin-mediated endocytosis may be the most common endocytic pathway for AgNPs in B16 cells, and AgNPs at each size were likely to enter cells by a major internalization pathway.

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Section: Introductionmentioning

confidence: 99%

<p>Size-dependent cellular uptake and localization profiles of silver nanoparticles</p>

Guo

Liu

et al. 2019

IJN

222

118

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“…Silver NPs has been shown to be treated for wound healing, active against resistant pathogens, and cancer ,. Zinc oxide NPs from B. megaterium was reported to exert oxidative stress mediated toxicity …”

Section: Introductionmentioning

confidence: 99%

Biocompatible Tin Oxide Nanoparticles: Synthesis, Antibacterial, Anticandidal and Cytotoxic Activities

et al. 2019

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Biocompatible SnO 2 NPs, were synthesized by simple and facile ultrasonic technique, and further studied for antibacterial, anticandidal and invitro cytotoxic activity. The obtained size of synthesized SnO 2 NPs, was 5-30 nm and characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM). The evaluation of antibacterial and anticandidal activity of the SnO 2 NPs, was done by determining the minimum inhibitory concentration (MIC), and minimum bactericidal/fungicidal concentration (MBC/MFC), against Escherichia coli and Candida albicans, respectively. The MIC of 0.5 mg/ml and MBC of > 1 mg/ml for E. coli, while as the MIC of 8 mg/ml and MFC > 16 mg/ml for C. albicans, was obtained. Maximum activity for both, bacteria and Candida was achieved, however, to the best of our knowledge, this is the first report of anticandidal activity exhibited by SnO 2 NPs. Treatment of synthesized SnO 2 NPs against the colorectal (HCT-116) cancer cell line, decreased the cell survivability in a dose-dependent manner, as lower dose showed decrease of 73.00%, whereas, higher dosage caused a significant 31.34% decrease in the cell viability. The obtained results confirmed, that the prepared SnO 2 NPs can be the future broad-spectrum antibacterial, anticandidal and anticancer agent, and can be further explored for application in the biomedical field.promising potential for use in the pharmaceutical and biomedical applications. Supporting information summaryThe experimental section includes the detailed procedure for synthesis, characterization, antibacterial, anticandidal and cytotoxic activities of Tin Oxide NPs (SnO 2 NPs), which can be find in supporting information.

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“…Among other metal nanoparticles, synthesis of silver nanoparticles (AgNPs) via biological mean gain wide acceleration due to their profound applications in medical application, textile dye removal, wastewater treatment, biological sensors etc. [16,[20][21][22]. Synthesis of AgNPs via fungi gain more attention because they produce large amount of biomass in a short period of time under lab conditions and produce large amount of extracellular protein that help in synthesis of nanoparticles [23,24].…”

Section: Introductionmentioning

confidence: 99%

Extracellular synthesis of silver nanoparticles using entomopathogenic fungus: characterization and antibacterial potential

Tyagi¹,

Tyagi²,

Gola³

et al. 2019

SN Appl. Sci.

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Present study involves the simple, rapid, non-toxic and in vitro method of extracellular silver nanoparticles synthesis using Entomopathogenic fungus (Beauveria bassiana). The development of silver nanoparticle in fungal supernatant was confirmed by the absorbance peak at 450 nm in UV-Vis spectrophotometer. Further, presence of AgNPs and its crystal lattice was confirmed by EDS and XRD, respectively. TEM micrograph confirmed the presence of differently shaped (triangular, circular, hexagonal) nanoparticles with size ranging from 10 to 50 nm. Variable shape and size of fungal assisted AgNps was also confirmed in SEM study. The optimal pH and temperature for biosynthesis of nanoparticles was found to be 6.0 and 25 °C, respectively. The continuous effects of AgNPs against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus in time dependent manner was confirmed in growth kinetic studies. During 36 h of growth study, maximum reduction in O.D 560 was found in E. coli (67.2%) followed by P. aeruginosa (63.3%) and S. aureus (56.8%) at 30 °C. The MIC values of fungal assisted AgNPs against E. coli, P. aeruginosa and S. aureus was found to be 2.5, 3 and 4.5 ppm, respectively. The MIC values of Ciprofloxacin was observed to be 0.5, 0.5 and 0.7 ppm, whereas MICs of AgNPs + Ciprofloxacin showed 0.4, 0.4, 0.5 ppm against E. coli, P. aeruginosa and S. aureus, respectively, clearly highlighting the synergistic effect of AgNPs in combination with Ciprofloxacin. In the view of challenges for developing antimicrobial nanoparticles of variable shape and size by various other methods, tuning nanoparticles synthesis via fungi can be a wonderful approach to resolve existing hurdles.

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Synthesis of silver nanoparticles from Phenerochaete chrysosporium (MTCC-787) and their antibacterial activity against human pathogenic bacteria

Cited by 229 publications

References 34 publications

<p>Size-dependent cellular uptake and localization profiles of silver nanoparticles</p>

<p>Size-dependent cellular uptake and localization profiles of silver nanoparticles</p>

Biocompatible Tin Oxide Nanoparticles: Synthesis, Antibacterial, Anticandidal and Cytotoxic Activities

Extracellular synthesis of silver nanoparticles using entomopathogenic fungus: characterization and antibacterial potential

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