Synthesis of Silver Nanoparticles from Sargassum Tenerrimum and Screening Phytochemicals for Its Antibacterial Activity

Kumar, Pradeep; Selvi, S Senthamil; Prabha, A. Lakshmi; Kumar, Krishna; Ganeshkumar, Rajasekaran; Govindaraju, M.

doi:10.5101/nbe.v4i1.p12-16

Cited by 107 publications

(50 citation statements)

References 22 publications

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“…The UVvisible absorption spectrum of the reaction mixture was analyzed at different wavelengths ranging from 400 to 700 nm. The characteristic absorption peak at 450 nm in UV-Visible spectrum confirmed the formation of AgNPs [16,17]. The broad peak indicates the presence of nanoparticles with a large size distribution as well as polydispersity nature of the particle [18].…”

Section: Discussionmentioning

confidence: 81%

“…A strong absorption peak at 3,430 cm -1 indicates the presence of phenols and alcohols with the free O-H group. The peak at 1,644 cm -1 represents the presence of amide I group and may well arise due to the carbonyl stretch in proteins [17]. Absorbance bands were seen at 3410 cm -1 were assigned to the stretching vibrations of primary and secondary amines, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Green Synthesis, Characterization of Silver Nanoparticles of a Marine Red Alga Spyridia Fusiformis and Their Antibacterial Activity

Murugesan¹,

Bhuvaneswari²,

Sivamurugan³

2017

Int J Pharm Pharm Sci

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Objective: In the present system, the green synthesis of silver nanoparticles using marine the red alga Spyridia fusiformis and antibacterial activity was carried out. Methods:The seaweed extract was used for the synthesis of AgNPs Results:The UV-visible spectroscopy revealed surface plasmon resonance at 450 nm. The FT-IR measurements showed the possible functional groups responsible for the formation of nanoparticles. The X-ray diffraction analysis showed that the particles were crystalline in nature. TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5-50 nm. The silver nanoparticles synthesized from the S. fusiformis showed higher activity and proved their efficacy in controlling the pathogenic bacterial strains. The nanoparticles showed highest inhibition activity on K. pneumaniae and S. aureus up to 26 and 24±0.01 mm at 100 μg/ml of nanoparticles.at room temperature. The silver nanoparticles were characterized by using UVVisible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscope and X-ray diffraction (XRD) techniques. The antibacterial activity of biosynthesized silver nanoparticles was carried out by disc diffusion method against pathogenic bacteria. Conclusion:The synthesised AgNPs have shown the best antibacterial activity against human pathogens Keywords: Antibacterial activity, Green synthesis, Marine algae, Silver nanoparticles, Spyridia fusiformis E. coli, K. pneumoniae, S. aureus and P. aeruginosa. The above eco-friendly AgNPs synthesis procedure could be a viable solution for industrial applications in the future and therapeutic needs.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Green Synthesis, Characterization of Silver Nanoparticles of a Marine Red Alga Spyridia Fusiformis and Their Antibacterial Activity

Murugesan¹,

Bhuvaneswari²,

Sivamurugan³

2017

Int J Pharm Pharm Sci

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“…The observed band in this range has been associated with Ag-NPs confirming the synthesis of spherical Ag-NPs with narrow size distribution (Kumar et al, 2012). The microstructure characterization of Ag-NPs was carried out using SEM showed that the Ag-NPs were in narrow size range, with most of the particles with size of 32 and 43nm.…”

Section: Discussionmentioning

confidence: 94%

Potential antibacterial activity of crude extracts and silver nanoparticles synthesized from Sargassum wightii

Thirumalairaj¹,

Vijayan²,

Durairaj³

et al. 2014

Int Curr Pharm J

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The present work investigates the antibacterial activity of silver nanoparticles (Ag-NPs) synthesized by biological method using Sargassum wightii. The fresh live seaweed was collected from the Mandapam coast of Tamilnadu, India. Solvent extract was prepared using acetone, petroleum ether and methanol. Aqueous extract of the seaweed was also used for the synthesis of silver Ag-NPs. Seaweed extract is used as a reducing agent of 2mM silver nitrate solution for the synthesis of Ag-NPs. Periodical monitoring of reaction mixture was done using UV-vis spectroscopy at 300-750 nm. The scanning electron microscopy (SEM) of the sample confirms the presence of Ag-NPs. The antibacterial activity of solvent extract was done by Minimal inhibitory concentration (MIC) assay. The methanol extract of the seaweed at a concentration of 250µg/ml exhibited potent antimicrobial activity against the test microorganism. The zone of inhibition ranging from 8-14 mm was observed with different extracts. The antibacterial activity of the synthesized Ag-NPs against the organism was also done by MIC test. The MIC of Ag-NPs was found to be 130µg/ml for all pathogenic microorganisms selected for the study. The zone of inhibition against Bacillus cereus, Bacillus anhtracis, Staphylococcus aureus and Vibrio alginoyticus were found to be 10, 8, 10 and 9 mm, respectively. The synthesized Ag-NPs exhibited significant antimicrobial activity against the selected microorganisms than the solvent extract of seaweed.DOI: http://dx.doi.org/10.3329/icpj.v3i10.20337 International Current Pharmaceutical Journal, September 2014, 3(10): 322-325

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“…In this study, we have synthesized silver nanoparticles rapidly at 15 minutes using the extract of P. tetrastromatica. Similarly, Kumar et al [12] synthesized silver nanoparticles within 20 minutes by using the extract of Sargassum tenerrimum and Singaravelu et al, [7] synthesized gold nanoparticles after the 24 hrs incubation time using Sargassum wightii.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Characterization of Silver Nanoparticles From Marine Brown Seaweed and Its Antifungal Efficiency Against Clinical Fungal Pathogens

Rajeshkumar¹,

Malarkodi²,

S³

2017

Asian J Pharm Clin Res

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Objectives: The aim of this study is to synthesize silver nanoparticles using the algal extract of Padina tetrastromatica and evaluate its antifungal activity against pathogenic fungus isolated from clinical samples.Methods: Dried algal biomass was used to prepare the pure algal extract and added with 1 mM AgNO 3 , and the color change was noted and recorded by ultraviolet (UV)-vis spectrophotometer. The morphological characteristics were analyzed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Crystalline structure was analyzed by SAED pattern. Antifungal activity was performed by agar well diffusion method against various pathogenic fungi.Results: Formation of brown color at 15 minutes indicates the production of silver nanoparticles by the extract of brown algae P. tetrastromatica. Surface plasmon resonance band was centered at 440 nm which was observed by UV-vis spectrophotometer. SEM image revealed spherical and cubical nanoparticles with high agglomeration, and energy-dispersive X-ray illustrates elemental components of silver formed at 3 keV. TEM shows spherical, truncated, and ellipsoidal nanoparticles and also it evidences the algae compounds that are capped with nanoparticles. SAED pattern proved four diffraction face-centered cubic rings at (111), (200), (220), and (311) which indicates the crystalline nature of nanoparticles. Silver nanoparticles show high inhibition activity against Fusarium sp, Aspergillus niger, Candida albicans, Aspergillus fumigatus, and Aspergillus flavus at different concentrations. P. tetrastromatica-mediated synthesis of silver nanoparticles shows rapid and eco-friendly silver ion reduction process. Conclusion:Therefore, this present study elucidates that algae-mediated synthesized silver nanoparticles have antifungal activity against pathogenic fungi, so it can be developed as a novel medicine for human welfare in biomedical applications in the near future.

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Synthesis of Silver Nanoparticles from Sargassum Tenerrimum and Screening Phytochemicals for Its Antibacterial Activity

Cited by 107 publications

References 22 publications

Green Synthesis, Characterization of Silver Nanoparticles of a Marine Red Alga Spyridia Fusiformis and Their Antibacterial Activity

Green Synthesis, Characterization of Silver Nanoparticles of a Marine Red Alga Spyridia Fusiformis and Their Antibacterial Activity

Potential antibacterial activity of crude extracts and silver nanoparticles synthesized from Sargassum wightii

Synthesis and Characterization of Silver Nanoparticles From Marine Brown Seaweed and Its Antifungal Efficiency Against Clinical Fungal Pathogens

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