Synthesis of stoichiometric nano crystalline hydroxyapatite by ethanol-based sol–gel technique at low temperature

Kuriakose, T.Anee; Kalkura, S. Narayana; Palanichamy, M.; Arivuoli, D.; Dierks, Karsten; Bocelli, G.; Betzel, C.

doi:10.1016/j.jcrysgro.2003.11.057

Cited by 250 publications

(116 citation statements)

References 36 publications

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“…62 The intense peaks located at 1032, 605, and 565 cm −1 can be attributed to the PO 4 3− . 63 It should be noted that the similar result that the characteristic stretching mode of the -OH vibration was overlapped with the strong band of the adsorbed water has been observed in a previous study on HA. 64 The relative intensity of the peak at about 3540 cm −1 decreased after the calcination of the sample at 800°C for 3 hours (see (D) in Figure 3), compared with that in (C).…”

Section: Resultssupporting

confidence: 84%

Hierarchically nanostructured hydroxyapatite: hydrothermal synthesis, morphology control, growth mechanism, and biological activity

2012

IJN

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Hierarchically nanosized hydroxyapatite (HA) with flower-like structure assembled from nanosheets consisting of nanorod building blocks was successfully synthesized by using CaCl 2 , NaH 2 PO 4 , and potassium sodium tartrate via a hydrothermal method at 200°C for 24 hours. The effects of heating time and heating temperature on the products were investigated. As a chelating ligand and template molecule, the potassium sodium tartrate plays a key role in the formation of hierarchically nanostructured HA. On the basis of experimental results, a possible mechanism based on soft-template and self-assembly was proposed for the formation and growth of the hierarchically nanostructured HA. Cytotoxicity experiments indicated that the hierarchically nanostructured HA had good biocompatibility. It was shown by in-vitro experiments that mesenchymal stem cells could attach to the hierarchically nanostructured HA after being cultured for 48 hours. Objective: The purpose of this study was to develop facile and effective methods for the synthesis of novel hydroxyapatite (HA) with hierarchical nanostructures assembled from independent and discrete nanobuilding blocks. Methods: A simple hydrothermal approach was applied to synthesize HA by using CaCl 2 , NaH 2 PO 4 , and potassium sodium tartrate at 200°C for 24 hours. The cell cytotoxicity of the hierarchically nanostructured HA was tested by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Results: HA displayed the flower-like structure assembled from nanosheets consisting of nanorod building blocks. The potassium sodium tartrate was used as a chelating ligand, inducing the formation and self-assembly of HA nanorods. The heating time and heating temperature influenced the aggregation and morphology of HA. The cell viability did not decrease with the increasing concentration of hierarchically nanostructured HA added. Conclusion: A novel, simple and reliable hydrothermal route had been developed for the synthesis of hierarchically nanosized HA with flower-like structure assembled from nanosheets consisting of nanorod building blocks. The HA with the hierarchical nanostructure was formed via a soft-template assisted self-assembly mechanism. The hierarchically nanostructured HA has a good biocompatibility and essentially no in-vitro cytotoxicity.

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Section: Resultssupporting

confidence: 84%

Hierarchically nanostructured hydroxyapatite: hydrothermal synthesis, morphology control, growth mechanism, and biological activity

2012

IJN

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“…[3][4][5][6] Therefore, several methods have been used to prepare hydroxyapatite, including solid state reactions, plasma techniques, crystal growth under hydrothermal conditions, layer hydrolysis of other calcium phosphate salts, and the sol-gel route. [7][8][9][10][11][12][13][14] Among these methods, hydrothermal synthesis has received more attention because of its well known inherent advantages, such as low energy consumption, high product purity, homogeneous composition, and better control over size and shape.…”

Section: Introductionmentioning

confidence: 99%

Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities

Liu

Zhao

et al. 2012

IJN

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Introduction:Hydroxyapatite (HA) is the principal inorganic constituent of human bone. Due to its good biocompatibility and osteoconductivity, all kinds of HA particles were prepared by different methods. Numerous reports demonstrated that the properties of HA affected its biological effects. Methods: Two kinds of nanohydroxyapatite with different sizes and crystallinities were obtained via a hydrothermal treatment method under different temperatures. It was found that at a temperature of 140°C, a rod-like crystal (n-HA1) with a diameter of 23 ± 5 nm, a length of 47 ± 14 nm, and crystallinity of 85% ± 5% was produced, while at a temperature of 80°C, a rod-like crystal (n-HA2) with a diameter of 16 ± 3 nm, a length of 40 ± 10 nm, and crystallinity of 65% ± 3% was produced. The influence of nanohydroxyapatite size and crystallinity on osteoblast viability was studied by MTT, scanning electron microscopy, and flow cytometry. Results: n-HA1 gave a better biological response than n-HA2 in promoting cell growth and inhibiting cell apoptosis, and also exhibited much more active cell morphology. Alkaline phosphatase activity for both n-HA2 and n-HA1 was obviously higher than for the control, and no significant difference was found between n-HA1 and n-HA2. The same trend was observed on Western blotting for expression of type I collagen and osteopontin. In addition, it was found by transmission electron microscopy that large quantities of n-HA2 entered into the cell and damaged the cellular morphology. Release of tumor necrosis factor alpha from n-HA2 was markedly higher than from n-HA1, indicating that n-HA2 might trigger a severe inflammatory response. Conclusion: This work indicates that not all nanohydroxyapatite should be considered a good biomaterial in future clinical applications.

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“…Sol-gel method Sol-gel method, one of the widely used wet chemical methods in synthesizing various nanomaterials [15,[73][74][75], has been used for fabrication of ZnO QDs [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40]. Among a number of reports on fabrication of ZnO QDs over the past decades, sol-gel method was the mostly used one due to its simplicity and cheapness.…”

Section: Chemical Methods On Synthesis Of Zno Qdsmentioning

confidence: 99%

Synthesis, Self-assembly and Optoelectronic Properties of Monodisperse ZnO Quantum Dots

Mei¹,

Hu²

2011

Optoelectronic Devices and Properties

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Synthesis of stoichiometric nano crystalline hydroxyapatite by ethanol-based sol–gel technique at low temperature

Cited by 250 publications

References 36 publications

Hierarchically nanostructured hydroxyapatite: hydrothermal synthesis, morphology control, growth mechanism, and biological activity

Hierarchically nanostructured hydroxyapatite: hydrothermal synthesis, morphology control, growth mechanism, and biological activity

Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities

Synthesis, Self-assembly and Optoelectronic Properties of Monodisperse ZnO Quantum Dots

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