“…Alkaline protease from a alkalophilic strain, bacillus pseudofirmus AL-89 has no catalytic activity in THF, acetone, dioxan, acetonitrile or all of these mixed with DMF(1 : 1, v/v), while it has been shown to catalyze the acylation of sucrose at the 2-O-position and 1 -O-position in pyridine, DMF, DMF-DMSO (1 : 1, v/v). 20 But these highly polar organic solvents are toxic to the enzymes. 10,25 For lipases, both good solubility of the substrate and the high enzymatic activity was hardly obtained by using one organic solvent alone.…”