Synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis

Guilherme, Ederson Paulo Xavier; Oliveira, Jacqueline Pawlowski; Carvalho, Lorendane Millena de; Brandi, Igor Viana; Santos, Sérgio Henrique Sousa; Carvalho, Gleidson Giordano Pinto de; Cota, Júnio; Carvalho, Bruna Mara Aparecida de

doi:10.1002/elps.201700208

Cited by 10 publications

(2 citation statements)

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“…The effect of different experimental conditions (pH, temperature, concentration of starch solution) on starch hydrolysis were investigated. The results obtained in this study supports that the alpha-amylase immobilized cryogels are applicable for industrial processes [61]. In a study carried out by Jain, E. et al, different cryogels consist of polyacrylamide-chitosan (PAAC), poly(NIPA)-chitosan, polyacrylonitrile, and poly(NIPA) were investigated for hybridoma cell (6A4D7, B7B10, and H9E10) growth and monoclonal antibody (mAb) production.…”

Section: Bioreactorssupporting

confidence: 80%

Biomedical Applications of Polymeric Cryogels

et al. 2019

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The application of interconnected supermacroporous cryogels as support matrices for the purification, separation and immobilization of whole cells and different biological macromolecules has been well reported in literature. Cryogels have advantages over traditional gel carriers in the field of biochromatography and related biomedical applications. These matrices nearly mimic the three-dimensional structure of native tissue extracellular matrix. In addition, mechanical, osmotic and chemical stability of cryogels make them attractive polymeric materials for the construction of scaffolds in tissue engineering applications and in vitro cell culture, separation materials for many different processes such as immobilization of biomolecules, capturing of target molecules, and controlled drug delivery. The low mass transfer resistance of cryogel matrices makes them useful in chromatographic applications with the immobilization of different affinity ligands to these materials. Cryogels have been introduced as gel matrices prepared using partially frozen monomer or polymer solutions at temperature below zero. These materials can be produced with different shapes and are of interest in the therapeutic area. This review highlights the recent advances in cryogelation technologies by emphasizing their biomedical applications to supply an overview of their rising stars day to day.

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Section: Bioreactorssupporting

confidence: 80%

Biomedical Applications of Polymeric Cryogels

et al. 2019

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“…The realization of all high‐performance liquid chromatography (HPLC) modes for polymer monolithic sorbents appeared to be possible because of relative simplicity of their synthesis and covalent modification needed for adsorptive functionalization 8,9 . Despite the recent developments of novel kinds of monoliths (soft gel monoliths, 10 superporous cryogels, 11 inorganic, 12 and hybrid monoliths, 13 high internal phase emulsion monoliths, 14 etc.) rigid macroporous polymer materials still dominate 15–19 .…”

Section: Introductionmentioning

confidence: 99%

Some factors affecting pore size in the synthesis of rigid polymer monoliths: Theory and its applicability

Korzhikova-Vlakh

Tennikova

2021

J of Applied Polymer Sci

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Macroporous monoliths represent now widely used materials whose successful application strongly depends on their characteristics. Among those the average pore size is one of the key parameters. In this paper, we consider the applicability of theoretical calculations for the selection of appropriate porogens to generate the materials with required average pore size. A set of macroporous poly(meth)acrylate monoliths was synthesized via thermo‐ and photo‐initiated free radical polymerization and characterized in regards to their average pore size. Additionally, the difference in solubility parameters as well as Hansen's solubility parameter distance between monomers and porogens were calculated for each polymerization mixture using Hildebrand's and Hansen's solubility theories. The theoretical predications and experimental data were compared and analyzed to establish the applicability of theoretical calculations to previse average pore size for different systems. It was found that Hildebrand's theory seems to be poorly appropriate as universal tool, while Hansen's theoretical approach explained better the efficiency of solvents as porogens. The application of oligomers and polymer solutions due to the increase of viscosity as well as the variation of crosslinker amount in the monomer system can be singled out as Hansen's theory limitations at the prediction of the average pore size.

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The role of laboratory-scale bioreactors at the semi-continuous and continuous microbiological and biotechnological processes

Tikhomirova

Taraskevich

Ponomarenko

2018

Appl Microbiol Biotechnol

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Laboratory-scale bioreactors represent an important part of microbiological, biotechnological, and biochemical researches. The small volume bioreactors enable the biomass growth, extracellular metabolite production, solid-state fermentation, and biocatalytic processes in a controlled open system. Using the lab-scale bioreactors, the processes which were optimized in experiments can be scaled up to a pilot level. In turn, the manufacturing scale processes can be scaled down to study the heterogeneity of the system and its effect on the producer. This mini-review is focused on a short description of features of the most popular designs of continuous and semi-continuous bioreactors applied at the present time as well as their application area.

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Synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis

Cited by 10 publications

References 33 publications

Biomedical Applications of Polymeric Cryogels

Biomedical Applications of Polymeric Cryogels

Some factors affecting pore size in the synthesis of rigid polymer monoliths: Theory and its applicability

The role of laboratory-scale bioreactors at the semi-continuous and continuous microbiological and biotechnological processes

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