Synthesis of surface imprinted polymers based on wrinkled flower-like magnetic graphene microspheres with favorable recognition ability for BSA

Pang

et al. 2022

Analyst

Section: Preparation and Surface Modification Of Mmipsmentioning

confidence: 99%

Section: Selective Adsorption Of Biomarkers By Mmipsmentioning

confidence: 99%

Application progress of magnetic molecularly imprinted polymers chemical sensors in the detection of biomarkers

Wang

Pang

et al. 2022

Analyst

“…In response to the above problems, researchers have continuously improved the preparation process and imprinting methods of MIPs. Consequently, a series of novel protein-imprinting methods and strategies have been developed, including surface imprinting on nanomaterials, − smart material molecular imprinting, − epitope imprinting, − and metal-chelating imprinting. − The imprinted sites of the molecularly imprinted polymer prepared by surface imprinting on the nanomaterial are located on the surface of the carriers, which is conducive to the elution and binding of the template molecules. So, this imprinting method has the characteristics of low mass transfer resistance and fast adsorption rate.…”

Section: Introductionmentioning

confidence: 99%

Cobalt-Iron Double Ion-Bovine Serum Albumin Chelation-Assisted Thermo-Sensitive Surface-Imprinted Nanocage with High Specificity

ACS Appl. Mater. Interfaces

Zhang

Ren

et al. 2021

Self Cite

To develop multifunctional protein imprinted materials, a cobalt-iron double ion-BSA directional chelation-assisted thermo-sensitive surface-imprinted hollow nanocage (Co-Fe@CBMA-MIPs) with excellent specificity is developed on the surface of ZIF-67@Co-Fe in this study by synergizing the advantages of surface imprinting, metal ion chelation, anti-protein adsorption segments, and thermo-sensitive components. Beyond previous research, well-designed multifunctional protein-imprinted materials possess high binding capacity, fast adsorption kinetics, and outstanding selectivity. When the adsorption is carried out at 32 °C, the adsorption capacity of Co-Fe@CBMA-MIPs for BSA reaches 520.35 mg/g within 50 min. The imprinting factor is 8.55. The selectivity factors of Co-Fe@CBMA-MIPs for HSA, Bhb, OVA, and Lyz are 3.72, 6.09, 4.10, and 8.41, respectively. More significantly, Co-Fe@CBMA-MIPs could specifically recognize BSA from mixed proteins and actual samples and exhibit excellent repeated use stability. Based on the above advantages, the development of this research provides an effective means to improve the recognition specificity of molecularly imprinted polymers.

“…Their saturated adsorption amount was 21.7 mg/g, and the imprinting factor (IF) was 4.16. the reason for the higher IF is that the surface area was fully used for the template, thereby nonspecific adsorption was reduced. Yang et al 27 used ultrasonic atomization to prepare magnetic graphene microspheres with wrinkled surfaces. By using polydopamine (PDA) and BSA as the imprinting polymer and the template molecule, respectively, the adsorption capacity of Fe 3 O 4 @ rGO@MIPs for BSA could reach 317.58 mg/g in 60 min.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Surface-Imprinted Magnetic Wrinkled Microspheres and Their Specific Adsorption of BSA

Zhang

Ren

et al. 2021

Ind. Eng. Chem. Res.

Self Cite

Increasing the external surface area of carriers has great significance in the production of protein-imprinted polymers because it can simultaneously increase the mass-transfer rate and adsorption capacity. In this paper, magnetic composite microspheres with a wrinkled surface (MWMS) are fabricated by the combination of controlled polymerization and interfacial polymerization. Their high surface area and excellent magnetic properties make them an advantageous carrier. The surface of MWMS is coated with polydopamine that served as an imprinting layer via oxidative autopolymerization of dopamine in alkaline solution, to obtain bovine serum albumin (BSA) surface-imprinted MWMS (MWMS@MIPs). MWMS@MIPs can adsorb 146.2 mg/g BSA in 60 min, and the imprinting factor (IF) is 4.36. The adsorption process conforms to the Langmuir adsorption model revealing the monolayer adsorption. The calculated maximum saturated adsorption amount is close to the measured value of 206.61 mg/g. More importantly, BSA can be specifically discriminated from the mixed protein and real samples using MWMS@MIPs. The regeneration experiment of eight cycles illustrates that the adsorption capacity, IF, and magnetic properties of MWMS@MIPs basically remain unchanged. MWMS@MIPs are excellent and promising materials for separating proteins.