Synthesis of the extracellular protease byBacillus pumilus

Fabián, J.

doi:10.1007/bf02873079

Cited by 17 publications

(2 citation statements)

References 18 publications

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“…It has been known for many years that Bacillus pumilus produces extracellular proteases (Fabian, 1970). However, it was not until 1997 that a serine alkaline protease, named protease Q, was first purified from B. pumilus (Han and Damodaran, 1997).…”

Section: Introductionmentioning

confidence: 99%

Salt stress represses production of extracellular proteases in Bacillus pumilus

Liu¹,

Huang²,

Hong³

2015

Genet. Mol. Res.

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ABSTRACT.Bacillus pumilus is able to secrete subtilisin-like proteases, one of which has been purified and characterized biochemically, demonstrating great potential for use in industrial applications. In the current study, the biosynthesis and transcription of extracellular proteases in B. pumilus (BA06) under salt stress were investigated using various methods, including a proteolytic assay, zymogram analysis, and real-time PCR. Our results showed that total extracellular proteolytic activity, both in fermentation broth and on milk-containing agar plates, was considerably repressed by salt in a dosage-dependent manner. As Bacillus species usually secret multiple extracellular proteases, a variety of individual extracellular protease encoding genes were selected for real-time PCR analysis. It was shown that proteases encoded by the aprE and aprX genes were the major proteases in the fermentation broth in terms of their transcripts in B. pumilus. Further, transcription of aprE, aprX, and epr genes was indeed repressed by salt stress. In contrast, transcription of other genes (e.g., vpr and wprA) was not repressed or significantly affected by the salt. Conclusively, salt stress represses total extracellular proteolytic activity in B. pumilus, which can largely be ascribed to suppression of the major protease-encoding genes (aprE, R.F. Liu et al. 4940©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 4939-4948 (2015) aprX) at the transcriptional level. In contrast, transcription of other protease-encoding genes (e.g., vpr, wprA) was not repressed by salt stress.

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Section: Introductionmentioning

confidence: 99%

Salt stress represses production of extracellular proteases in Bacillus pumilus

Liu¹,

Huang²,

Hong³

2015

Genet. Mol. Res.

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“…In some cases, process conditions can be adjusted in a favorable way and can cause an increase of enzyme concentration and productivity (1,5,7). In other cases, batch production is superior to continuous production in terms of product concentration (6,8,16,22). Besides product concentration and productivity, the long-term sta-bility of production is decisive for the application of continuous culture.…”

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confidence: 99%

Continuous Production of Thermostable β-Amylase with Clostridium thermosulfurogenes : Effect of Culture Conditions and Metabolite Levels on Enzyme Synthesis and Activity

Nipkow

Shen

Zeikus

1989

Appl Environ Microbiol

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A ,-amylase-overproducing mutant of Clostridium thermosulfurogenes was grown in continuous culture on soluble starch to produce thermostable I-amylase. Enzyme productivity was reasonably stable over periods of weeks to months. The pH and temperature optima for I-amylase production were pH 6.0 and 60°C, respectively. Enzyme concentration was maximized by increasing biomass concentration by using high substrate concentrations and by maintaining a low growth rate. I8-Amylase concentration reached 90 U ml-' at a dilution rate of 0.07 h-1 in a 3% starch medium. A further increase in enzyme activity levels was limited by acetic acid inhibition of growth and low ,I-amylase productivity at low growth rates.

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Autolysis of disintegrated cells of the yeast saccharomyces cerevisiae

Běehalová

Beran

1986

Acta Biotechnologica

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Optimum conditions for autolysis of disintegrated cells of S. cerecisiae are at 50-53°C and pH 5.5; the process is terminated after 6 h. In the presence of sodium chloride (3-5'7') the autolysis is complete after 5 h. The yield of autolysis of disintegrated yeast cells is about 70% of autolytic product per yeast dry weight. The product obtained after centrifugation, filtration and drying has very good sensoric and physical properties.

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Synthesis of the extracellular protease byBacillus pumilus

Cited by 17 publications

References 18 publications

Salt stress represses production of extracellular proteases in Bacillus pumilus

Salt stress represses production of extracellular proteases in Bacillus pumilus

Continuous Production of Thermostable β-Amylase with Clostridium thermosulfurogenes : Effect of Culture Conditions and Metabolite Levels on Enzyme Synthesis and Activity

Autolysis of disintegrated cells of the yeast saccharomyces cerevisiae

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