Synthesis of the myelin proteolipid protein in the developing mouse brain

Campagnoni, Anthony T.; Hunkeler, Markus J.

doi:10.1002/neu.480110403

Cited by 42 publications

(22 citation statements)

References 24 publications

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“…mRNA in the brain are low at 3 days of age, and they reach a peak over a broad period from about 14 to 25 days. This closely correlates with the time of active myelination and the most active period of PLP synthesis (5). Even the adult mouse maintains approximately one-fourth to one-third of the maximal PLP mRNA levels, suggesting that myelin protein synthesis and replacement is continuing in the adult.…”

Section: Discussionmentioning

confidence: 55%

“…It is of interest that these data contrast with observations on the active synthesis of the two proteins in brain-slice experiments. The peak of active synthesis of MBP occurs between 16 and 18 days of age in the mouse, and the peak of active synthesis of PLP occurs at approximately 22 days of age (5). This apparent discrepancy may result from the fact that different methodologies were utilized in the two sets of experiments.…”

Section: Discussionmentioning

confidence: 99%

“…The normal pattern of myelin protein expression in rodents indicates that little myelin protein expression occurs prenatally or in neonatal animals (2,5,6,8,23,26,28) and that myelin protein synthesis and accumulation increase significantly in the brain at about 10 days after birth, reaching a peak between 15 and 22 days after birth (2,5,6,8,23).…”

mentioning

confidence: 99%

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Characterization of myelin proteolipid mRNAs in normal and jimpy mice.

Gardinier¹,

Macklin²,

Diniak³

et al. 1986

Mol. Cell. Biol.

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A clone specific for the rat myelin proteolipid protein (PLP) was isolated from a cDNA library made in pUC18 from 17-day-old rat brain stem mRNA. This clone corresponded to the carboxyl-terminal third of the PLP-coding region. The clone was used to identify PLP-specific mRNAs in mouse brain and to establish the time course of PLP mRNA expression during mouse brain development. Three PLP-specific mRNAs were seen, approximately 1,500, 2,400, and 3,200 bases in length, of which the largest was the most abundant. During brain development, the maximal period of PLP mRNA expression was from 14 to 25 days of age, and this was a similar time course to that for myelin basic protein mRNA expression. When the jimpy mouse, an X-linked dysmyelination mutant, was studied for PLP mRNA expression, low levels of PLP mRNA were seen which were approximately 5% of wild-type levels at 20 days of age. When jimpy brain RNA was analyzed by Northern blotting, the PLP-specific mRNA was shown to be 100 to 200 bases shorter than the wild-type PLP-specific mRNA. This size difference was seen in the two major PLP mRNAs, and it did not result from a loss of polyadenylation of these mRNAs.The biosynthesis of the myelin sheath is a crucial part of nervous system development, and as a result, the developmental expression of the myelin protein genes is highly regulated. The normal pattern of myelin protein expression in rodents indicates that little myelin protein expression occurs prenatally or in neonatal animals (2,5,6,8,23,26,28) and that myelin protein synthesis and accumulation increase significantly in the brain at about 10 days after birth, reaching a peak between 15 and 22 days after birth (2,5,6,8,23).Central nervous system myelin contains two major classes of proteins, myelin basic protein (MBP) and proteolipid protein (PLP), which together constitute as much as 80% of the total myelin protein (15,30). The four MBPs that are present in rodent myelin have homologous amino acid sequences, including identical amino-and carboxyl-terminal sequences but internal segments of unique amino acid sequence (3). They are translated from four different mRNAs (50), and the four MBP mRNAs can be produced from a single MBP gene by alternative mRNA splicing (12,44). Two PLPs are present in myelin, the major PLP, which has an apparent molecular weight of 25,000, and the closely related proteolipid DM20 (1), which has an apparent molecular weight of 20,000. PLP and DM20 may have a relationship similar to that found among the four MBPs, since they have identical amino-and carboxyl-terminal sequences (46), and they are very closely related by amino acid composition and by antigenic characteristics. The expression of these myelin proteins correlates closely with the formation of myelin in the developing rat brain, and mouse mutants that are defective in myelin formation show lowered levels of these myelin proteins (4,14,20,25 expression and the genetic deficiency in the shiverer mouse myelination mutant (12,34,44). Several groups have recently isolated cDNA ...

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

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Characterization of myelin proteolipid mRNAs in normal and jimpy mice.

Gardinier¹,

Macklin²,

Diniak³

et al. 1986

Mol. Cell. Biol.

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“…To the best of our knowledge, a developmental study to estab lish the timing of maximal cellular expres sion of PLP or MAG in neonatal cultures has not so far been reported. With respect to these two markers the cultures appeared to reflect the in vivo order of expression since, in vivo, MAG has been reported to reach maximal levels before MBP [40] and MBP before PLP [34][35][36],…”

Section: Discussionmentioning

confidence: 99%

Temporal Expression of Myelin-Specific Components in Neonatal Mouse Brain Cultures: Evidence that 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase Appears Prior to Galactocerebroside

Amur‐Umarjee¹,

Dasu²,

Campagnoni³

1990

Dev Neurosci

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A range of antibodies to cell-specific markers was employed to characterize and quantify the cell types present in neonatal mouse brain cell cultures. Astrocytes were the major cell type to develop in culture and formed a bed layer by 5–7 days in vitro (DIV). A population of cells appeared on this layer which consisted of oligodendrocytes, progenitor cells and microglia. Some neurons were detected in the cultures up to at least 27 DIV. With time, the number of progenitor cells decreased in the cultures and there was a concomitant increase in the number of oligodendrocytes. Maximal numbers of cells expressing galactocerebroside (GC), 2'',3''-cyclic nucleotide 3''-phosphodiesterase (CNP) and myelin-associated glycoprotein were observed at 18 DIV. The highest number of cells expressing myelin basic protein and proteolipid protein were observed at 25–30 and 35–40 DIV, respectively. Double-label studies with antibodies to A2B5/CNP and A2B5/GC showed that several A2B5⁺, CNP⁺ cells were present at 6 DIV. In contrast, no A2B5⁺, GC⁺ cells could be seen at this age. Confirming these findings, some CNP⁺.GC^– cells were observed when cells were double-labeled with antibodies to CNP and GC. These findings suggested that the expression of CNP precedes that of GC in oligodendrocytes in these cultures.

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“…The appearance of myelin proteins during brain development has primarily been studied by gel electro phoresis of isolated myelin or of individual proteins isolated by extraction of whole brain tissue [3][4][5][6][7][8][9], Several studies have utilized the specificity of immunologic techniques in ra dioimmunoassays (RIA) for myelin basic protein [10][11][12][13][14][15][16][17]. The present studies were ini tiated to investigate myelination using an electroblot procedure to assess the appear-ance of both the proteolipid and the basic protein in whole tissue homogenates from different brain regions.…”

Section: Introductionmentioning

confidence: 99%

Electroblot Analysis of Rat Myelin Proteolipid Protein and Basic Protein during Development

1983

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The accumulation of the myelin proteolipid protein during rat brain development was studied in the spinal cord, lower brain stem (pons and medulla), cerebellum, and upper brain stem. Proteolipid accumulation was assessed on electroblots of total membrane samples from each region between 2 and 18 days of age. Proteolipid could be detected in rat spinal cord as early as 2 days of age, in lower brain stem and cerebellum by 4 days of age and in upper brain stem by 10 days of age. The time course of myelin basic protein accumulation was essentially the same as that of the proteolipid in each of the brain regions.

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Synthesis of the myelin proteolipid protein in the developing mouse brain

Cited by 42 publications

References 24 publications

Characterization of myelin proteolipid mRNAs in normal and jimpy mice.

Characterization of myelin proteolipid mRNAs in normal and jimpy mice.

Temporal Expression of Myelin-Specific Components in Neonatal Mouse Brain Cultures: Evidence that 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase Appears Prior to Galactocerebroside

Electroblot Analysis of Rat Myelin Proteolipid Protein and Basic Protein during Development

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