Synthesis of thermo- and acid-stable novel oligosaccharides by using dextransucrase with high concentration of sucrose

Seo, Eun-Seong; Nam, Seung‐Hee; Kang, Hyung Koo; Cho, Jae-Yong; Lee, Hee-Seon; Ryu, Hwa-Won; Kim, Doman

doi:10.1016/j.enzmictec.2006.08.017

Cited by 44 publications

(19 citation statements)

References 22 publications

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“…Stereoselective tris-glycosylation to introduce β-(1→3)-branches into gentiotetraose (Son, et al, 2008) Sialyl Lewis x MALDI Neu5Ac-α -(2→3)-Gal building block Sialylated pentasaccharides MALDI, ESI Related to avian and human influenza virus infection Sulfhydryl-containing ethyleneglycol-derivatized oligosaccharides TOF For conjugation with ovalbumin for targeting to dendritic cells Thermo and acid stable TOF (DHB) Synthesised with dextransucrase with high levels of sucrose (Seo, et al, 2007a) Various TOF Use of n-pentenylorthoesters having manno-, gluco-or galacto-configurations in regioselective glycosylations (Jayaprakash & Fraser-Rei, 2007) Xylo-gluco-oligosaccharides TOF As probes for α-xylosyltransferases (Fauré, et al, 2007) Xylooligosaccharides TOF Use of Geobacillus stearothermophilus GH52 β-xylosidase…”

Section: Maldi-ftms (No Matrix)mentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

Harvey

2011

Mass Spectrometry Reviews

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This review is the fifth update of the original review, published in 1999, on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2008. The first section of the review covers fundamental studies, fragmentation of carbohydrate ions, use of derivatives and new software developments for analysis of carbohydrate spectra. Among newer areas of method development are glycan arrays, MALDI imaging and the use of ion mobility spectrometry. The second section of the review discusses applications of MALDI MS to the analysis of different types of carbohydrate. Specific compound classes that are covered include carbohydrate polymers from plants, N- and O-linked glycans from glycoproteins, biopharmaceuticals, glycated proteins, glycolipids, glycosides and various other natural products. There is a short section on the use of MALDI mass spectrometry for the study of enzymes involved in glycan processing and a section on the use of MALDI MS to monitor products of the chemical synthesis of carbohydrates with emphasis on carbohydrate-protein complexes and glycodendrimers. Corresponding analyses by electrospray ionization now appear to outnumber those performed by MALDI and the amount of literature makes a comprehensive review on this technique impractical. However, most of the work relating to sample preparation and glycan synthesis is equally relevant to electrospray and, consequently, those proposing analyses by electrospray should also find material in this review of interest.

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Section: Maldi-ftms (No Matrix)mentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

Harvey

2011

Mass Spectrometry Reviews

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“…Dextransucrase (EC 2.4.1.5) is the extracellular glycosyltransferase produced by this strain. The same enzyme is also responsible for the synthesis of prebiotic oligosaccharides through the acceptor reaction (Pereira et al 1998;Rabelo et al 2006;Rodrigues et al 2006;Fernandes and Rodrigues 2006;Seo et al 2007) L. mesenteroides NRRL B-512F is the main strain used in industrial fermentations to produce dextransucrase and dextran. The enzyme expression is inducible and, nowadays, is industrially obtained by fermentation of L. mesenteroides NRRL B-512F in a synthetic medium containing only sucrose as carbon source.…”

Section: Introductionmentioning

confidence: 98%

Dextransucrase Stability in Cashew Apple Juice

Honorato

Rodrigues

2008

Food Bioprocess Technol

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The use of agriculture waste as substrate for biotechnological process became an interesting alternative to reduce production costs and the negative impact of agricultural wastes disposal in the environment. In Brazil, cashew tree cultivation is done aiming the cashew nut production, and tons of cashew apples are wasted in the field after the nut removal. Thus, cashew apple juice can be used as low cost substrate for biotechnological process. In this study, the stability of dextransucrase produced in a fermentation medium using cashew apple juice as substrate was evaluated. The crude enzyme was stable at 30°C for 30 h at pH ranging from 4.5 to 5.5. The partially purified enzyme was stable in non-fermented cashew apple juice at pH 5.0 for 96 h at 30°C. The high stability of the enzyme in cashew apple juice at room temperature allows its industrial use without needing the enzyme purification, consequently reducing process costs.

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“…Hence, reducing sucrose content in juice is beneficial in keeping blood glucose level of consumers stable, and in preventing these metabolic syndromes [ 14 ]. One approach is to convert sucrose into fructo-oligosaccharides (FOSs) through the action of extracellular/intracellular fructosyltransferases (FTases, EC 2.4.1.9) or β-fructofuranosidases (FFases, EC 3.2.1.26) [ 15 , 16 , 17 ], or into gluco-oligosaccharides (GOSs) by glucansucrases [ 18 ]. As prebiotics, consumption of FOSs and GOSs, as well as other oligosaccharides, could modulate gut microbiota [ 19 , 20 ], help to create a healthy intestinal ecology [ 21 ] and improve mineral absorption in the colon [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides

et al. 2018

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The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated. The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment. The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro. The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment. The water-soluble polysaccharide content, including pectin, was significantly increased. Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content. Substantial fructo-oligosaccharides including 1-kestose and nystose were synthesized after enzyme treatment. The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment. The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.

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Synthesis of thermo- and acid-stable novel oligosaccharides by using dextransucrase with high concentration of sucrose

Cited by 44 publications

References 22 publications

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

Dextransucrase Stability in Cashew Apple Juice

Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides

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