Synthesis of thiodisaccharides related to 4-thiolactose. Specific structural modifications increase the inhibitory activity against E. coli β-galactosidase

Abstract

Searching for new glycosidase inhibitors, a set of benzyl β-D-Gal-S-(1→4)-3-deoxy-4-thio-α-D-hexopyranosides was synthesized. Diverse configurations have been installed at C-2 and C-4 of the glucose residue. The benzyl glycosidic group was...

Reutilization of the Most Stable Coimmobilized Enzyme Using Glutaraldehyde Chemistry to Produce a New Combi-biocatalyst When the Coimmobilized Enzyme with a Lower Stability Is Inactivated

“…One likely application may be the simultaneous modification of the fats and lactose of milk to produce free-lactose milk (or lactose enriched in prebiotic galactosides) − that could also bear flavors produced by the lipase action, decrease the triglyceride concentration, − or be protected by the presence of some free fatty acids with antimicrobial agent activity. − Other application may be the production of galactosyl-diglycerides using lipase to produce specific diglycerides from the corresponding triglyceride (e.g., 1,2 diacetin) and the β-galactosidase from A. oryzae as the catalyst to produce the O-link ,− between galactose and diglyceride via kinetically controlled synthesis. − In this instance, the high concentration inside the particle of the diglyceride may be critical to have good reaction yields, and the immediate modification can prevent the fact that the acyl migration results in 1,3 diacetin …”