SYNTHESIS OF THYMOSIN β₄

Abstract

4. Heyes

The regenerative peptide thymosin β4 accelerates the rate of dermal healing in preclinical animal models and in patients

“…Previously reported nonclinical studies suggest that Tβ4 may have the biological properties found useful clinically in these patient populations. Tβ4 is a highly conserved naturally occurring, water‐soluble peptide 2,3 . In addition to being found in a majority of tissues and cell types, it is also found in the blood, and in other body fluids, including wound fluids, tears, saliva, and cerebrospinal fluid 4–6 .…”

TheEscherichia coli-Derived Thymosinβ4 Concatemer Promotes Cell Proliferation and Healing Wound in Mice

“…T β 4 protein is therefore likely to have increasing importance as a therapeutic agent, which in turn suggests that there will be an increasing demand for T β 4 production. Currently, the T β 4 is produced commercially by two approaches: extraction from animal thymus [1] and chemical synthesis [25], but there are challenges with meeting clinic demand due to risks of zoonosis and higher production costs [25, 26]. …”

Thymosin β4 and its posttranslational modifications

“…It was suspected that thymosin β 4 would be an important immunomodulatory factor influencing maturation of T‐lymphocytes. Therefore, the acetylated tritetracontapeptide was synthesized already in 1981 by automated solid phase synthesis 3 . Because thymosin fraction V consisted of many major and minor peptides a nomenclature was proposed based on the isoelectric points of the various sequenced peptides.…”

“…Therefore, the acetylated tritetracontapeptide was synthesized already in 1981 by automated solid phase synthesis. 3 Because thymosin fraction V consisted of many major and minor peptides a nomenclature was proposed based on the isoelectric points of the various sequenced peptides. Peptides with isoelectric points below 5 were termed ␣-thymosins (highly acidic) and those with isoelectric points between 5 and 7 (acidic) were named ␤-thymosins.…”